Analysis of invasive and metastatic potential using visible oral carcinogenesis model

• Project/Area Number: 22890225
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Hyogo Medical University
• Principal Investigator: ZUSHI Yusuke 兵庫医科大学, 医学部, 助教 (80581206)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,782,000 (Direct Cost: ¥2,140,000、Indirect Cost: ¥642,000); Fiscal Year 2011: ¥1,326,000 (Direct Cost: ¥1,020,000、Indirect Cost: ¥306,000); Fiscal Year 2010: ¥1,456,000 (Direct Cost: ¥1,120,000、Indirect Cost: ¥336,000)
• Keywords: 口腔がん / 発がん / HPV / erbB1 / EGFR

Research Abstract

We have developed for the first time an in vitro culture model faithfully recapitulating the development of HPV-positive and negative oral squamous cell carcinomas with genetically defined elements using normal human tongue keratinocytes(HTKs).

Research Products

• [Journal Article] An in vitro multistep carcinogenesis model for both HPV positive and negative human oral squamous cell carcinoma (2011)
  • Author(s): Yusuke Zushi, Mako Narisawa-Saito, Kazuma Noguchi, Yuki Yoshimatsu, Takashi Yugawa, Nagayasu Egawa, Shin-ichi Ohno, Masatoshi Fujita, Masahiro Urade and Tohru Kiyono.
  • Journal Title: Am J Can Re Volume: 1(7) Pages: 869-881
  • NAID: 130004707939
  • URL: http://www.ajcr.us/ISSN:2156-6976/ajcr0000079
  • Peer Reviewed
• [Journal Article] An in vitro multistep carcinogenesis model for both HPV positive and negative human oral squamous cell carcinoma (2011)
  • Author(s): Yusuke Zushi
  • Journal Title: American journal of cancer research Volume: 1(7) Pages: 869-81
  • NAID: 130004707939
  • Peer Reviewed
• [Journal Article] 口腔粘膜の多段階発がんとヒトパピローマウイルス(HPV) (2010)
  • Author(s): 頭司雄介, 野口一馬, 浦出雅裕
  • Journal Title: 口腔組織培養学会誌 Volume: 19(2) Pages: 19-27
  • Peer Reviewed
• [Presentation] 不死化ヒト正常舌ケラチノサイト(HTK)を用いた口腔扁平上皮がんの多段階発がんの機構解析 (2011)
  • Author(s): 頭司雄介, 野口一馬, 山村倫世, 高岡一樹, 瀬川英美, 岸本裕充, 浦出雅裕
  • Organizer: 第46回日本口腔組織培養学会学術総会
  • Place of Presentation: 千葉
  • Year and Date: 2011-11-19
• [Presentation] 不死化ヒト正常舌ケラチノサイト(HTK)を用いた口腔扁平上皮がんの多段階発がんの機構解析 (2011)
  • Author(s): 頭司雄介
  • Organizer: 第48回日本口腔組織培養学会・総会
  • Place of Presentation: 浦安
  • Year and Date: 2011-11-19
• [Presentation] An in vitro multistep carcinogenesis model for both HPV positive and negative human oral squamous cell carcinoma (2011)
  • Author(s): Yusuke Zushi, Kazuma Noguchi, Michiyo Yamamura, Kazuki Takaoka, Kuniyasu Moridera, Emi Segawa, Shin Okui, Hiromitsu Kishimoto, Masahiro Urade
  • Organizer: 20th International Conference Oral Maxillofacial Surgery(ICOMS2011)
  • Place of Presentation: Santiago, CHILE
  • Year and Date: 2011-11-02
• [Presentation] An in vitro multistep carcinogenesis model for both HPV positive and negative human oral squamous cell carcinoma (2011)
  • Author(s): Yusuke Zushi
  • Organizer: 20^<th> Inational conference on oral and maxillofacial surgery (ICOMS20th)
  • Place of Presentation: Santiago CHILE
  • Year and Date: 2011-11-02
• [Presentation] 不死化ヒト正常舌ケラチノサイト(HTK)を用いた口腔扁平上皮がんの多段階発がんの機構解析 (2011)
  • Author(s): 頭司雄介
  • Organizer: 第57回日本口腔外科学会総会・学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-21
• [Presentation] 不死化ヒト正常舌ケラチノサイト(HTK)を用いた口腔扁平上皮がんの多段階発がんの機構解析 (2010)
  • Author(s): 頭司雄介, 野口一馬, 山村倫世, 高岡一樹, 森寺邦康, 瀬川英美, 奥井森, 岸本裕充, 浦出雅裕
  • Organizer: 第56回日本口腔外科学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-10-16
• [Presentation] 口腔癌細胞におけるEMT markerとしてのzyxinの発現 (2010)
  • Author(s): 瀬川英美、野口一馬、山村倫世、高岡一樹、頭司雄介、岸本裕充、橋谷進、浦出雅裕
  • Place of Presentation: 千葉
  • Year and Date: 2010-10-16
• [Presentation] 口腔癌細胞におけるEMTmarkerとしてのzyxinの発現 (2010)
  • Author(s): 頭司雄介
  • Organizer: 第55回日本口腔外科学会総会・学術大会
  • Place of Presentation: 千葉
  • Year and Date: 2010-10-16
• [Presentation] 不死化ヒト正常舌ケラチノサイト(HTK)を用いた口腔扁平上皮がんの多段階発がんの機構解析 (2010)
  • Author(s): 頭司雄介、齋藤真子、野口一馬、吉松有紀、稲川悠紀、温川恭至、江川長靖、大野真一、藤田雅俊、浦出雅裕、清野透
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-24
• [Presentation] 不死化ヒト正常舌ケラチノサイト(HTK)を用いた口腔扁平上皮がんの多段階発がんの機構解析 (2010)
  • Author(s): 頭司雄介
  • Organizer: 第69回日本癌学会総会・学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-22
• [Presentation] An in vitro multistep carcinogenesis model for both HPV positive and negative human oral squamous cell carcinoma (2010)
  • Author(s): Yusuke Zushi, Mako Narisawa-Saito, Yuki Yoshimatsu, Takashi Yugawa, Nagayasu Egawa, Shin-ichi Ohno, Masatoshi Fujita, Kazuma Noguchi, Masahiro Urade and Tohru Kiyono
  • Organizer: 26th International Pappillomavirus Conference & Clinical and Public Health Workshops(IPV2010)
  • Place of Presentation: Montreal
  • Year and Date: 2010-07-05
• [Presentation] An in vitro multistep carcinogenesis model for both HPV positive and negative human oral squamous cell carcinoma (2010)
  • Author(s): Yusuke Zushi
  • Organizer: 26th International Pappillomavirus Conference & Clinical and Public Health Workshops (IPV2010)
  • Place of Presentation: Montreal, CANADA
  • Year and Date: 2010-07-04