Epithelium-intrinsic microRNAs contribute to the mucosal immune homeostasis through ensuring epithelial cell differentiation
| Project/Area Number |
22890238
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
NAKATO Gaku 独立行政法人理化学研究所, 免疫系構築研究チーム, 特別研究員 (20584535)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000)
Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000)
Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
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| Keywords | microRNA / 杯細胞 / M細胞 |
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| Research Abstract |
To elucidate the mechanisms of intestinal epithelial cell differentiation and homeostasis by microRNA(miRNA), we performed microarray analysis of the goblet cell differentiated colon cancer cell line HT29-MTX and intestinal epithelium from control mice or intestinal epithelial cell specific deletion of Dicer1(Dicer-KO) mice. The data sets were analyzed by Ingenuity Pathway Analysis software and miRNA target prediction database. We found several microRNAs that might be involved in goblet cell or M cell differentiation. In addition, phenotype analysis of Dicer-KO mice indicated that these mice might be useful as an ulcerative colitis model.
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Report
(3 results)
Research Products
(9 results)
