Epithelium-intrinsic microRNAs contribute to the mucosal immune homeostasis through ensuring epithelial cell differentiation

• Project/Area Number: 22890238
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: NAKATO Gaku 独立行政法人理化学研究所, 免疫系構築研究チーム, 特別研究員 (20584535)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000); Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000); Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
• Keywords: microRNA / 杯細胞 / M細胞

Research Abstract

To elucidate the mechanisms of intestinal epithelial cell differentiation and homeostasis by microRNA(miRNA), we performed microarray analysis of the goblet cell differentiated colon cancer cell line HT29-MTX and intestinal epithelium from control mice or intestinal epithelial cell specific deletion of Dicer1(Dicer-KO) mice. The data sets were analyzed by Ingenuity Pathway Analysis software and miRNA target prediction database. We found several microRNAs that might be involved in goblet cell or M cell differentiation. In addition, phenotype analysis of Dicer-KO mice indicated that these mice might be useful as an ulcerative colitis model.

Research Products

• [Journal Article] Expression pattern changes and function of RANKL during mouse lymph node microarchitecture development (2012)
  • Author(s): Machiko Sugiyama, Gaku Nakato, Toshi Jinnohara, Hisaya Akiba, Ko Okumura, Hiroshi Ohno, and Hisahiro Yoshida
  • Journal Title: Int Immunol
  • Peer Reviewed
• [Presentation] Cellular prion protein on Peyer's patch M cells serves as an invasive receptor for Brucella abortus (2012)
  • Author(s): Gaku Nakato and Hiroshi Ohno
  • Organizer: 3rd Nagasaki Prion mini-symposium
  • Place of Presentation: Japan
  • Year and Date: 2012-01-29
• [Presentation] Identification of RNA aptamers targeting mouse GP2 protein by SELEX (2011)
  • Author(s): Misaho Hanazato, Gaku Nakato, Fumiko Nishikawa, Koji Hase, Satoshi Nishikawa, Hiroshi Ohno
  • Organizer: 第34回日本分子生物学学会
  • Place of Presentation: 横浜
• [Presentation] Cellular prion protein on Peyer's patch M cells serves as invasive receptor of a zoonotic pathogen Brucella abortus (2011)
  • Author(s): Gaku Nakato, Koji Hase, Michio Suzuki, Noriyuki Nishida, Motohiro Horiuchi, Manabu Ato and Hiroshi Ohno
  • Organizer: 第40回日本免疫学会総会・学術集会
• [Presentation] M細胞に発現する内在性プリオンタンパク質はBrucella abortusの取り込み受容体として機能する (2011)
  • Author(s): 中藤学、長谷耕二、鈴木道雄、木村昌伸、新竜一郎、西田教行、度会雅久、今岡浩一、大野博司
  • Organizer: 第9回感染症沖縄フォーラム
  • Place of Presentation: 沖縄
• [Presentation] Distinct miRNA expression profiles of Follicle-associated epithelium(FAE) and villous epithelium(VE) (2010)
  • Author(s): Gaku Nakato, Koji Hase and Hiroshi Ohno
  • Organizer: The 19th CDB Meeting RNA Sciences in Cell and Development Biology Kobe
  • Place of Presentation: Japan
• [Book] 分子消化器病 (2011)
  • Author(s): 大野博司、長谷耕二、中藤学
• [Book] 細胞工学 (2011)
  • Author(s): 長谷耕二、中藤学、大野博司
• [Book] 臨床免疫-アレルギー科 (2010)
  • Author(s): 大野博司、長谷耕二、中藤学