Evaluation of radiation-induced tumor cell alterations using manganese enhanced MRI

• Project/Area Number: 22890240
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Radiation science
• Research Institution: Osaka University (2011); National Institute of Radiological Sciences (2010)
• Principal Investigator: SAITO Shigeyoshi 大阪大学, 大学院・医学系研究科, 助教 (40583068)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,068,000 (Direct Cost: ¥2,360,000、Indirect Cost: ¥708,000); Fiscal Year 2011: ¥1,469,000 (Direct Cost: ¥1,130,000、Indirect Cost: ¥339,000); Fiscal Year 2010: ¥1,599,000 (Direct Cost: ¥1,230,000、Indirect Cost: ¥369,000)
• Keywords: 放射線治療 / マンガン造影MRI / 動物MRI / 腫瘍マウス / 細胞周期 / 細胞増殖能 / 細胞増職能 / T_1定量MRI / フローサイトメトリー

Research Abstract

The purpose of radiotherapy is to treat malignancies with the intention of destroying or inactivating cells. It is important to detect an early response of radiotherapy against tumor cells. We conducted the manganese-enhanced MRI(MEMRI) to detect radiation-induced cell cycle arrest. Mn^<2+> accumulation is decreased in radiation-exposed in-vitro/ vivo tumor cells with the cell cycle arrest. Our data provide evidence that MEMRI become a more specific in-vitro/ vivo tumor imaging of cell cycle alterations.

Research Products

• [Journal Article] Quantitative assessment of central nervous system disorder induced by prenatal X-ray exposure using diffusion and manganese-enhanced MRI (2012)
  • Author(s): Saito S, Aoki I, Sawada K, Suhara T.
  • Journal Title: NMR Biomed Volume: 25(1) Issue: 1 Pages: 75-83
  • DOI: 10.1002/nbm.1715
  • Peer Reviewed
• [Journal Article] Brain N-acetylaspartate is increased in mice with hypomyelination (2012)
  • Author(s): Takanashi J, Saito S, Aoki I, Barkovich J, Itoh Y, Inoue K.
  • Journal Title: J Magn Reson Imaging Volume: 35 Issue: 2 Pages: 418-425
  • DOI: 10.1002/jmri.22817
  • Peer Reviewed
• [Journal Article] Tumor Enhancement Effect of Overexpressed Manganese-superoxide Dismutase in Manganese-enhanced MR Imaging (2011)
  • Author(s): Sumitaka Hasegawa, Shigeyoshi Saito, Michiko Koshikawa-Yano, Takako Furukawa, Ichio Aoki and Tsuneo Saga
  • Journal Title: Magnetic Resonance in Medical Sciences Volume: 10 Issue: 3 Pages: 155-158
  • DOI: 10.2463/mrms.10.155
  • NAID: 10029698456
  • ISSN: 1347-3182, 1880-2206
  • Peer Reviewed
• [Journal Article] Quantitative and noninvasive assessment of prenatal X-ray irradiation-induced CNS abnormalities using magnetic resonance imaging (2011)
  • Author(s): Shigeyoshi Saito, Ichio Aoki, Kazuhiko Sawada, Xue-Zhi Sun, Kai-Hsiang Chuang, Jeff Kershaw, Iwao Kanno and Tetsuya Suhara
  • Journal Title: Radiation Research Volume: 175(1) Issue: 1 Pages: 1-9
  • DOI: 10.1667/rr2134.1
  • Peer Reviewed
• [Journal Article] Tumor enhancement effect of over expressed manganese-superoxide dismutase in manganese-enhanced magnetic resonance imaging (2011)
  • Author(s): Sumitaka Hasegawa, Shigeyoshi Saito, Michiko Koshikawa-Yano, Takako Furukawa, Ichio Aoki, Tsuneo Saga
  • Journal Title: Magnetic Resonance in Medical Sciences Volume: 10(3) Pages: 155-158
  • Peer Reviewed
• [Journal Article] Spatial frequency-based analysis of mean red blood cell speed in single microvessels : investigation of microvascular perfusion in rat cerebral cortex (2011)
  • Author(s): Autio J, Kawaguchi H, Saito S, Aoki I, Obata T, Masamoto K, Kanno I
  • Journal Title: PLoS ONE Volume: 6 Issue: 8 Pages: e24056-e24056
  • DOI: 10.1371/journal.pone.0024056
  • Peer Reviewed
• [Journal Article] Quantitative and noninvasive assessment of prenatal X-ray irradiation-induced CNS abnormalities using magnetic resonance imaging (2011)
  • Author(s): Shigeyoshi Saito, Ichio Aoki, Kazuhiko Sawada, Xue-Zhi Sun, Kai-Hsiang Chuang, Jeff Kershaw, Iwao Kanno, Tetsuya Suhara.
  • Journal Title: Radiation Research Volume: 175 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Quantitative assessment of CNS disorder induced by prenatal X-ray exposure using diffusion and manganese-enhanced MRI (MEMRI) (2011)
  • Author(s): Shigeyoshi Saito, Ichio Aoki, Kazuhiko Sawada, Tetsuya Suhara.
  • Journal Title: NMR in biomedicine Volume: In press
  • Peer Reviewed
• [Journal Article] Molecular imaging of mesothelioma by detection of manganese-superoxide dismutase activity using manganese-enhanced magnetic resonance imaging (2010)
  • Author(s): Sumitaka Hasegawa, Michiko Koshikawa-Yano, Shigeyoshi Saito, Yukie Morokoshi, Takako Furukawa, Ichio Aoki and Tsuneo Saga
  • Journal Title: International Journal of Cancer Volume: 128(9) Issue: 9 Pages: 2138-46
  • DOI: 10.1002/ijc.25547
  • Peer Reviewed
• [Journal Article] Molecular imaging of mesothelioma by detection of manganese-superoxide dismutase activity using manganese-enhanced magnetic resonance imaging (2010)
  • Author(s): Sumitaka Hasegawa, Michiko Koshikawa-Yano, Shigeyoshi Saito, Yukie Morokoshi, Takako Furukawa, Ichio Aoki, Tsuneo Saga
  • Journal Title: International Journal of Cancer Volume: 128 Pages: 2138-2146
  • Peer Reviewed
• [Presentation] ^1H-MRSを用いた重粒子線照射後の急性期における中枢神経細胞変性評価 (2011)
  • Author(s): 齋藤茂芳、高梨潤一、佐賀恒夫、青木伊知男
  • Organizer: 第39回日本磁気共鳴医学会大会
  • Place of Presentation: 小倉
  • Year and Date: 2011-09-30
• [Presentation] MRI/ MRSを用いた重粒子線照射後の中枢神経細胞障害の評価 (2011)
  • Author(s): 齋藤茂芳、高梨潤一、佐賀恒夫、青木伊知男
  • Organizer: 第6回分子イメージング学会学術大会
  • Place of Presentation: 神戸
  • Year and Date: 2011-05-26
• [Presentation] MRSを用いた重粒子線照射後における中枢神経細胞の変性評価 (2011)
  • Author(s): 齋藤茂芳、高梨潤一、佐賀恒夫、青木伊知男
  • Organizer: 第67回日本放射線総合学術大会
  • Place of Presentation: Web開催
  • Year and Date: 2011-05-09
• [Presentation] Diffusion tensor imaging for evaluation of radiation-induced developmental abnormalities in the white matter (2011)
  • Author(s): Shigeyoshi Saito, Tsuneo Saga, Ichio Aoki
  • Organizer: 19th Scientific Meeting and Exhibition of the International Society for Magnetic Resonance in Medicine (ISMRM)
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2011-05-04
• [Presentation] In-vivo detection of cell cycle arrest using manganese-enhanced MRI (MEMRI) (2011)
  • Author(s): Shigeyoshi Saito, Sumitaka Hasegawa, Takako Furukawa, Tsuneo Saga, Ichio Aoki
  • Organizer: 19th Scientific Meeting and Exhibition of the International Society for Magnetic Resonance in Medicine (ISMRM)
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2011-05-04
• [Presentation] Brain N-acetylaspartate is Increased in Mice with Hypomyelination (2011)
  • Author(s): Jun-ichi takanashi, Shigeyoshi Saito, Ichio Aoki, A.J.Barkovich, Hitoshi Terada, Yukiko Ito, Ken Inoue
  • Organizer: 19th Scientific Meeting and Exhibition of the International Society for Magnetic Resonance in Medicine (ISMRM)
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2011-05-04
• [Presentation] マンガン増感MRIを用いた放射線照射による腫瘍細胞増殖能の評価 (2010)
  • Author(s): 齋藤茂芳、長谷川純崇、古川高子、菅野巌、佐賀恒夫、青木伊知男
  • Organizer: 第38回日本放射線技術学会秋季学術大会
  • Place of Presentation: 仙台
  • Year and Date: 2010-10-14
• [Presentation] 定量的マンガン増感MRIは、放射線照射後の早期の腫瘍細胞変性を検出する (2010)
  • Author(s): 齋藤茂芳、長谷川純崇、古川高子、菅野巌、佐賀恒夫、青木伊知男
  • Organizer: 第38回日本磁気共鳴医学会大会
  • Place of Presentation: つくば
  • Year and Date: 2010-09-30
• [Presentation] 定量的マンガン増感MRIは、放射線照射後の早期の腫瘍細胞変性を検出する (2010)
  • Author(s): 齋藤茂芳、長谷川純崇、古川高子、菅野巌、佐賀恒夫、青木伊知男
  • Organizer: 第39回日本磁気共鳴医学会大会
  • Place of Presentation: つくば
  • Year and Date: 2010-09-30
• [Presentation] Evaluation of radiotherapy using Manganese-enhanced MRI(MEMRI). (2010)
  • Author(s): Shigeyoshi Saito, Sumitaka Hasegawa, Takako Furukawa, TetsuyaSuhara, Iwao Kanno, Ichio Aoki.
  • Organizer: 18th Scientific Meeting and Exhibition of the International Society for Magnetic Resonance in Medicine(ISMRM).
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2010-05-04
• [Presentation] Manganese-enhanced MRI as a molecular imaging of mesothelioma. (2010)
  • Author(s): Sumitaka Hasegawa, Shigeyoshi Saito, Yukie Morokoshi, Takako Furukawa, Ichio Aoki, Tsuneo Saga.
  • Organizer: World Molecular Imaging Congress
  • Place of Presentation: Kyoto, Japan