Analysis of physi ological function for new peptides derived from VGF protein discovered by peptidomics analysis

• Project/Area Number: 22890253
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Endocrinology
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: MOCHIZUKI Akikazu 独立行政法人国立循環器病研究センター, 分子薬理部, 研究員 (30589601)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,068,000 (Direct Cost: ¥2,360,000、Indirect Cost: ¥708,000); Fiscal Year 2011: ¥1,469,000 (Direct Cost: ¥1,130,000、Indirect Cost: ¥339,000); Fiscal Year 2010: ¥1,599,000 (Direct Cost: ¥1,230,000、Indirect Cost: ¥369,000)
• Keywords: VGF / ペプチド / ノックアウトマウス / 生理活性ペプチド / ペプチドーム / 遺伝子欠損マウス

Research Abstract

In this study, we found NERP-1 and NERP-2 producing cells are colocalized with CRH immunopositive cells. We made antibodies against for NERP-3 and NERP-4.In addition, we made VGF knockout mouse. They are lean and inferility.

Research Products

• [Journal Article] Immunocytochemical localization of kisspeptin neurons in the rat forebrain with special reference to sexual dimorphism and interaction with GnRH neurons (2012)
  • Author(s): 徐枝芳、加賀重人、望月明和、坪水純、足立幸香、坂井貴文、井上金治、足立明人
  • Journal Title: Endocrine Journal Volume: 59(2) Pages: 161-171
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22240892
  • Peer Reviewed
• [Journal Article] Circadian transcriptional factor DBP regulates expression of Kiss in the anteroventral periventricular nucleus (2011)
  • Author(s): 徐枝芳、加賀重人、坪水純、藤崎潤、望月明和、坂井貴文、束村博子、前田敬一朗、井上金治、足立明人
  • Journal Title: Molecular and Cellular Endocrinology Volume: 339(1-2) Pages: 90-97
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21458520
  • Peer Reviewed
• [Journal Article] Circadian transcriptional factor DBP regulates expression of Kiss1 in the anteroventral periventricular nucleus (2011)
  • Author(s): Zhifang Xu
  • Journal Title: Molecular and Cellular Endocrinology Volume: 339(1-2) Pages: 90-7
  • Peer Reviewed
• [Presentation] The effects of prolactin-releasing peptide(PrRP) in energy metabolism and stress (2010)
  • Author(s): Mochizuki A
  • Organizer: 5^<th> International Peptide Symposium in conjunction with 47^<th> Japanese Peptide Symposium
  • Place of Presentation: Kyoto
• [Presentation] 時計遺伝子によるKiss1発現制御に関する研究 (2010)
  • Author(s): 徐枝芳
  • Organizer: 第17回日本時間生物学会
  • Place of Presentation: 東京
• [Presentation] プロラクチン放出ペプチド(PrRP)のストレス反応との関連 (2010)
  • Author(s): 望月明和
  • Organizer: 第28回日本内分泌代謝学サマーセミナー
  • Place of Presentation: 長崎
• [Presentation] Prolactin-Releasing Peptide(PrRP)のエネルギー代謝とストレスとの関連 (2010)
  • Author(s): 望月明和
  • Organizer: 第7回GPCR研究会
  • Place of Presentation: 東京