| Project/Area Number |
22F20708
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Review Section |
Basic Section 28050:Nano/micro-systems-related
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| Research Institution | The University of Tokyo |
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Principal Investigator |
竹内 昌治 東京大学, 生産技術研究所, 教授 (90343110)
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| Co-Investigator(Kenkyū-buntansha) |
LISI FABIO 東京大学, 生産技術研究所, 外国人特別研究員
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| Project Period (FY) |
2022-04-22 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2022: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Gold nanoparticles / hydrogel / controlled release / DNA / photothermal effect / microfluidic / cell culture / epithelial cells |
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| Outline of Research at the Start |
This research focuses of controlling the functional and biochemical properties of commercial hydrogels by using nanoparticles. These nanomaterials can be used for the localised delivery of signalling molecules that can influence cells embedded in the hydrogel, or as sensing elements to detect biomarkers of specific diseases. Delivery is triggered by using red or near-infrared light, a method that does not damage the cells and could potentially be used in-vivo. The results will correlate the dynamic changes in the hydrogel microenvironment to local composition, cell morphology and behaviour.
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| Outline of Annual Research Achievements |
The focus of my research was the development of nanoparticle systems that could be introduced into commercial hydrogels to tune their properties. One type of nanoparticle that I worked with are fluorescent quantum dots. To use these particles in hydrogel systems, it is essential to optimize their stability and optical properties in physiologically relevant conditions. I conducted a fundamental study, showing how previously published articles did not rigorously investigate the surface chemistry of these particles. The results of this investigation were published in the journal ACS Applied Nano Materials and will be presented to a conference in June 2023.
Another nanoparticle system that I continued developing was based on gold nanorods and DNA. Gold nanorods can very efficiently generate heat when irradiated by a red laser, and the resulting heat is transferred to the surrounding environment. When double strands of DNA are immobilized on the rods’ surface, the heat causes DNA melting and subsequent release of one strand into the surrounding media. This mechanism was confirmed using fluorophore-labelled DNA, and the plan was to use a laser for the targeted release of a growth factor in 3D cultures of endothelial cells. Endothelial cells spheroids were produced and used in a sprouting assay to test the release of growth factor from the Gold-DNA particles. More tests will be required to fully characterize the response of this system to laser irradiation and the resulting morphological changes on the endothelial cells.
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| Research Progress Status |
令和4年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和4年度が最終年度であるため、記入しない。
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