| Project/Area Number |
22H00360
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Medium-sized Section 38:Agricultural chemistry and related fields
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| Research Institution | Nagoya University |
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Principal Investigator |
Frommer Wolf 名古屋大学, トランスフォーマティブ生命分子研究所, 客員教授 (70795575)
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| Project Period (FY) |
2022-04-01 – 2025-03-31
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| Project Status |
Granted (Fiscal Year 2024)
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| Budget Amount *help |
¥42,510,000 (Direct Cost: ¥32,700,000、Indirect Cost: ¥9,810,000)
Fiscal Year 2024: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2023: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Fiscal Year 2022: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
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| Keywords | Phytohormone / Gibberelllic acid / Sugar / SWEETs / Transporter / Gibberellic acid |
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| Outline of Research at the Start |
GA and sugars can have synergistic effects on plant growth and yield, seed storage mobilization, fertility, and stress tolerance. The dual specificity of SWEETs for sugars and GA is possible nexus to understand GA and sugar interactions. We will here develop approaches and novel tools to dissect the contribution of sugars and GA and lay the basis for engineering improved plants with respect to the trails.
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| Outline of Annual Research Achievements |
Several SWEET transporters have been demonstrated to mediate transport of the plant hormone gibberellin (GA). The close physiological relationship between sucrose and GA raised the question of whether a functional interaction exists between these two substrates and whether they are both physiologically relevant. To address these inquiries, molecular docking and molecular dynamics simulations were conducted to predict the amino acids responsible for each substrate binding. Transport activity assays were then performed, which revealed mutants that selectively transport either sucrose or GA. These mutants were used to determine the physiological contribution of two different substrates facilitated by the same transporter. We found the sugar transport activity of SWEET13, not GA are responsible for pollen development.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Through the creation of substrate-specific transporters and their implementation in plant systems, our findings suggest that the sucrose transport functionality of SWEET13, rather than GA, plays a pivotal role in pollen development.
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| Strategy for Future Research Activity |
We will continue to explore the physiological relevance of sugar and GA transport activity of SWEETs. Additionally, we will endeavor to identify novel factors implicated in the governance of SWEET transport activity, with the object of comprehending the molecular mechanism of selectivity and activity regulation. Furthermore, we aim to develop a highly sensitive GA biosensor to facilitate an understanding of SWEET-mediated GA dynamics in planta. The signaling network of GA will also be investigated using a synthetic chemistry approach.
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