Adaptation of marine zooplankton to climate change conditions: a multi-omic study of molecular mechanisms.
Project/Area Number |
22H03795
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 64040:Social-ecological systems-related
|
Research Institution | Okinawa Institute of Science and Technology Graduate University |
Principal Investigator |
ラスカム ニコラス 沖縄科学技術大学院大学, ゲノム・遺伝子制御システム科学ユニット, 教授 (70724087)
|
Project Period (FY) |
2022-04-01 – 2025-03-31
|
Project Status |
Granted (Fiscal Year 2023)
|
Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2023: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2022: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
|
Keywords | Bioinformatics / genomics / climate change / marine biology / plankton / bioinformatics |
Outline of Research at the Start |
Oikopleura dioica is a zooplankton with exceptional thermal, pH and salt tolerance that thrives throughout the world’s oceans but the molecular mechanisms that enable such extensive adaptations are unknown. We will study how zooplanktons may adapt successfully to a rapidly changing climate.
|
Outline of Annual Research Achievements |
- We showed using molecular, morphological and reproductive analysis that the Okinawa Oikopleura dioica is probably a different species or sub-species to Osaka specimen (Masunaga et al, 2022). - We have recently completed the detailed genome comparisons between the Okinawa and Osaka Oikopleura dioica species (Plessy et al, Submitted). - We have established comparative assays to measure the development rate of Oikopleura dioica in different saline concentrations. - We have performed RNA-seq experiments of different time-points of exposure to different saline conditions.
|
Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
- We are currently analysing the RNA-seq data. - We are preparing to test further conditions (temperature, acidity). - Maintaining constant temperatures over long periods is currently a technical challenge.
|
Strategy for Future Research Activity |
- We are detecting clear phenotypic changes depending on the conditions. - Our main focus is to understand the gene regulation underlying the adaptation of behaviours. - Given the large number of cell types in an organism, we are currently testing single-cell RNA seq and cell line development.
|
Report
(1 results)
Research Products
(12 results)
-
-
[Journal Article] The cosmopolitan appendicularian Oikopleura dioica reveals hidden genetic diversity around the globe.2022
Author(s)
Masunaga A, Mansfield MJ, Tan Y, Liu AW, Bliznina A, Barzaghi P, Hodgetts TL, Ferrandez‐Roldan A, Canestro C, Onuma TA, Plessy C and Luscombe NM
-
Journal Title
Marine Biology
Volume: 169
Issue: 12
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-