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Novel strategies to expand the scope of biological nanopores for sensing biomolecules

Research Project

Project/Area Number 22K05330
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 37020:Chemistry and chemical methodology of biomolecules-related
Research InstitutionKagawa University

Principal Investigator

Kong Lingbing  香川大学, 農学部, 准教授 (20798384)

Project Period (FY) 2022-04-01 – 2027-03-31
Project Status Granted (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2026: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2025: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2024: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2023: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2022: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywordsnanopore
Outline of Research at the Start

Efficient discovery of new biological nanopores and conversion of existing ones into new ones is of most significance in technology development. The proposed multidisciplinary research program is to explore new strategies to expand the scope of biological nanopores for sensing biomolecules.

Outline of Annual Research Achievements

The establishments of the UPLC-MS system and the Benchtop NMR system have enabled the scale-up production of the essential chemicals. Various methods for the UPLC-MS system and the Benchtop NMR system have been generated and optimised. Large-scale expression of the target protein has also been achieved. The protocols for large-scale affinity chromatography, ion-exchange chromatography and size-exclusion chromatography with the AKTAgo FPLC system have been extensively explored and optimised. SDS-PAGE characterisation of the fractions from each chromatography purification gave the desired purity in good quantity. Thus, scale-up protein production and purification have also been achieved. The subsequent scale-up production of nanopores and follow-up purification is in progress. Various methods are being examined for the best results. Initial measurements with the relevant biophysical instruments are encouraging. Hopefully, desired nanopores with very high purity would be obtained soon so that the desired properties could be achieved.

Current Status of Research Progress
Current Status of Research Progress

3: Progress in research has been slightly delayed.

Reason

The delays in FY 2022 have led to slight delays in FY2023.

Strategy for Future Research Activity

The project is expected to progress smoothly from now on.

Report

(2 results)
  • 2023 Research-status Report
  • 2022 Research-status Report
  • Research Products

    (1 results)

All 2022

All Presentation (1 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Presentation] Multidisciplinary studies of the effect of capsular polysaccharide transporter inhibitors on plant bacterial pathogens2022

    • Author(s)
      KONG Lingbing
    • Organizer
      The 2nd Trilateral Symposium on SDGs 2022
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research / Invited

URL: 

Published: 2022-04-19   Modified: 2024-12-25  

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