| Project/Area Number |
22K05996
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 42020:Veterinary medical science-related
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| Research Institution | Nippon Veterinary and Life Science University |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2025-03-31
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| Project Status |
Completed (Fiscal Year 2024)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2024: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2023: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 乳腺腫瘍 / がん幹細胞 / 多様性 / 犬 / オルガノイド培養 / オルガノイド / spheres / 癌幹細胞 |
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| Outline of Research at the Start |
癌組織は自己複製能、多分化能、治療抵抗性を持つ癌幹細胞を根源とした不均一なかつ多様な細胞集団からなり、癌発症、再発、転移に重要な役割を担っている。犬の乳腺腫瘍は多様性を有し、腺上皮細胞の腫瘍性増殖に加え、筋上皮細胞増殖および骨・軟骨増殖を伴う特有の腫瘍組織を形成する。本研究は、生体で生じる癌組織を模倣したオルガノイド培養を用いて、犬に特有の乳腺腫瘍の発症・病態機構を解明する。さらに、獣医療における癌幹細胞標的治療法の基盤を構築し、獣医療における革新的な治療戦略を目指す。
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| Outline of Final Research Achievements |
The purpose of this study is to reveal the diversity of canine mammary tumors based on three-dimensional organoid cultures that mimic cancerous tissue arising in vivo. Surgically resected mammary tumors and their primary cultured cells were used for organoid and chondrogenic differentiation-induced cultures, and the formed organoids were analyzed for morphology and immunophenotype. Each tumor cultured in organoid culture formed spherical organoids. Organoids derived from benign mixed tumors of the mammary gland were also successfully cultured in chondrocyte-induced culture. Chondrocyte-induced culture resulted in a decrease in the number of cytokeratin 18-positive cells and an increase in the number of Cytokeratin 14-positive cells.
Translated with DeepL.com (free version)
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| Academic Significance and Societal Importance of the Research Achievements |
本研究成果は、犬に特有な乳腺腫瘍の発症・病態形成機構、多様性の解明に加え、乳癌幹細胞を標的とした新規癌治療戦略の基盤構築に貢献できる。さらに、ヒトのがん基礎研究だけでなく、ヒト医療への社会還元が期待できる極めて重要なトランスレーショナル研究である。
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