Elucidation of cancer mutagen by novel signature analysis

• Project/Area Number: 22K07164
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 50010:Tumor biology-related
• Research Institution: Chiba University
• Principal Investigator: 福世 真樹 千葉大学, 大学院医学研究院, 助教 (40639085)
• Co-Investigator(Kenkyū-buntansha): 大崎 敬子 杏林大学, 医学部, 教授 (90255406) ; 石川 健 久留米大学, 分子生命科学研究所, 助教 (10554040) ; 小林 一三 基礎生物学研究所, ゲノム情報研究室, 特別協力研究員 (30126057)
• Project Period (FY): 2022-04-01 – 2025-03-31
• Project Status: Granted (Fiscal Year 2023)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2024: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 癌 / 変異解析 / シグナチャー解析 / 胃癌 / ピロリ菌 / 塩基切り出し型制限酵素 / 変異シグナチャー / 発癌

Outline of Research at the Start

癌は主要疾患であり，何らかの要因で生じたゲノム変異の蓄積によって発生する.癌ゲ ノム変異の解析によって明らかになる特徴的な変異パターン(=変異シグナチャー)は発 癌の原因となる変異原を反映することが知られている.一方，未だ癌変異原の全貌は不明 であり，これが発癌メカニズムの理解，癌の多様性の克服や予防を阻んでいる.本研究で は新規癌変異原候補の同定を目的として，新規変異シグナチャー解析手法を開発する.さらに 既にこの手法による予備解析を行なって，胃癌の原因細菌であるピロリ菌の持つ塩基切り出し 型制限酵素PabIが変異原候補として挙っており，このPabIが胃癌変異を導入することを実証する.

Outline of Annual Research Achievements

従来の変異シグナチャーの拡張
(A-3)これまでに開発した3~7塩基パターンの癌変異中の濃縮を求めるプログラムによってTCGA(The Cancer Genome Atlas)からアクセス可能な全33癌種について全癌変異解析を行ない，各癌種毎に濃縮されたモチーフを明らかにした．さらにこれらのモチーフについてREBASE(Restriction Enzyme Database)と対応させ，その微生物の持つ制限酵素が変異源となり得る微生物種をリストした．このリストをBIC(database for the transcriptional landscape of bacteria in cancer)の各癌種毎に存在する微生物腫と統合することによって18癌種について延べ94微生物種が変異源候補として挙げられた．
胃癌変異源候補PabI
(A-7)これまでの研究から胃癌変異源候補としてあがっているピロリ菌の持つ制限酵素PabIについて，ピロリ菌のPabIの有無とホストであるヒトの胃癌発癌のリスクをHpGP株(Helicobacter pylori Genome Project)とNCBI登録株について解析したところ，ヨーロッパ株のピロリ菌感染者においてオッズ比2.5(p=0.00036)が得られた．さらに，解析対象をヨーロッパ株のあるsubpopulationに限定するとオッズ比5.0(p=0.017)が得られ，ピロリ菌のPabIと胃癌発癌の強い関連が示唆された．
現在，これらの結果について論文にまとめ，Nature Biotechnologyに投稿中である．

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

昨年度開発した新規癌変異シグナチャー解析手法を用いてTCGAの癌変異データを解析し，各癌種特異的な3~8塩基パターンを探索し制限酵素データベースREBASEに登録のある生物種-モチーフデータと対応させたところ，のべ103属の細菌に関連する265のシグナチャーが見つかった．さらにRNA-seq based メタゲノム解析データべースBICの各癌種毎に存在する微生物腫と統合することによって18癌種について延べ94微生物種を変異源候補として挙げた．
また，ピロリ菌の持つPabI制限酵素による胃癌変異導入仮説をより強固なものにするために，ピロリ菌側のPabIの有無とホストであるヒトの胃癌発癌の相関解析が必要となった．そこで申請時は計画されていなかったがHpGPとNCBI登録ピロリ菌株について，PabIの有無と胃癌発癌のリスク解析をしたところ，ヨーロッパ株のピロリ菌感染者においてオッズ比2.5(p=0.00036)が得られた．さらに，解析対象をヨーロッパ株のあるsubpopulationに限定するとオッズ比5.0(p=0.017)が得られ，ピロリ菌のPabIと胃癌発癌の強い関連が示唆された．
一方，大腸菌を用いた変異導入実験は，予定通りPabI変異生成系を改良し，変異導入実験，解析を行うことが出来たが，思うように変異が得られなかった.これに関しては変異解析手法を改良する，ヒト培養細胞での長期変異導入実験を行う等の改善や別のアプローチが必要である．

Strategy for Future Research Activity

ピロリ菌PabI制限酵素による胃癌変異導入仮説をより確かなものにするにはPabIによって実際にPabI認識配列関連変異が導入されていることを示す必要があるが，これまでの大腸菌を用いた変異導入実験では思うように結果が得られていない．そこで，ピロリ菌を多数株，経時的にヒトからサンプリングしたケースをデータベースから抽出し，そのゲノム比較によってピロリ菌におけるPabI認識配列関連変異導入を評価する．
さらに，変異導入実験についてはDuplex sequencing技術の導入によって変異検出感度を向上させる．同時に，ヒト胃上皮細胞でのPabI発現系の構築を行い，こちらのラインでも変異検出を試みる．

Research Products

• [Journal Article] Retrotransposons in Werner syndrome-derived macrophages trigger type I interferon-dependent inflammation in an atherosclerosis model (2024)
  • Author(s): Paul SK, Oshima M, Patil A, Sone M, Kato H, Maezawa Y, Kaneko H, Fukuyo M, Rahmutulla B, Ouchi Y, Tsujimura K, Nakanishi M, Kaneda A, Iwama A, Yokote K, Eto K, Takayama N
  • Journal Title: Nature Communications Volume: in press
  • Peer Reviewed / Open Access
• [Journal Article] Enhancer infestation drives tumorigenic activation of inactive B compartment in Epstein-Barr virus-positive nasopharyngeal carcinoma (2024)
  • Author(s): Mizokami Harue、Okabe Atsushi、Choudhary Ruchi、Mima Masato、Saeda Kenta、Fukuyo Masaki、Rahmutulla Bahityar、Seki Motoaki、Goh Boon-Cher、Kondo Satoru、Dochi Hirotomo、Moriyama-Kita Makiko、Misawa Kiyoshi、Hanazawa Toyoyuki、Tan Patrick、Yoshizaki Tomokazu、Fullwood Melissa Jane、Kaneda Atsushi
  • Journal Title: eBioMedicine Volume: 102 Pages: 105057-105057
  • DOI: 10.1016/j.ebiom.2024.105057
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Chromatin activation with H3K36me2 and compartment shift in metastatic castration-resistant prostate cancer (2024)
  • Author(s): Kanaoka Sanji、Okabe Atsushi、Kanesaka Manato、Rahmutulla Bahityar、Fukuyo Masaki、Seki Motoaki、Hoshii Takayuki、Sato Hiroaki、Imamura Yusuke、Sakamoto Shinichi、Ichikawa Tomohiko、Kaneda Atsushi
  • Journal Title: Cancer Letters Volume: 588 Pages: 216815-216815
  • DOI: 10.1016/j.canlet.2024.216815
  • Peer Reviewed / Open Access
• [Journal Article] Genome-Wide Analysis of DNA Methylation in Pseudomyxoma Peritonei Originated from Appendiceal Neoplasms (2024)
  • Author(s): Takane Kiyoko、Cai Tingwei、Noguchi Rei、Gohda Yoshimasa、Ikenoue Tsuneo、Yamaguchi Kiyoshi、Ota Yasunori、Kiyomatsu Tomomichi、Yano Hideaki、Fukuyo Masaki、Seki Motoaki、Bahityar Rahmutulla、Kaneda Atsushi、Furukawa Yoichi
  • Journal Title: Oncology Volume: - Issue: 8 Pages: 1-12
  • DOI: 10.1159/000536219
  • Peer Reviewed / Open Access
• [Journal Article] Peritumoral SPARC expression induced by exosomes from nasopharyngeal carcinoma infected Epstein‐Barr virus: A poor prognostic marker (2023)
  • Author(s): Dochi Hirotomo, Kondo Satoru, Komura Shigetaka, Moriyama‐Kita Makiko, Komori Takeshi, Nanbo Asuka, Sakaguchi Miako, Fukuyo Masaki, Hamabe‐Horiike Toshihide, Tanaka Mariko, Mizokami Harue, Kano Makoto, Kitagawa Yuki, Kobayashi Eiji, Hirai Nobuyuki, Ueno Takayoshi, Nakanishi Yosuke, et. al.
  • Journal Title: International Journal of Cancer Volume: 154 Issue: 5 Pages: 895-911
  • DOI: 10.1002/ijc.34777
  • Peer Reviewed / Open Access
• [Journal Article] The Link of mRNA and rRNA Transcription by PUF60/FIR through TFIIH/P62 as a Novel Therapeutic Target for Cancer (2023)
  • Author(s): Kitamura Kouichi、Hoshino Tyuji、Okabe Atsushi、Fukuyo Masaki、Rahmutulla Bahityar、Tanaka Nobuko、Kobayashi Sohei、Tanaka Tomoaki、Shida Takashi、Ueda Mashiro、Minamoto Toshinari、Matsubara Hisahiro、Kaneda Atsushi、Ishii Hideshi、Matsushita Kazuyuki
  • Journal Title: International Journal of Molecular Sciences Volume: 24 Issue: 24 Pages: 17341-17341
  • DOI: 10.3390/ijms242417341
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Helicobacter pylori Genome Project: insights into H. pylori population structure from analysis of a worldwide collection of complete genomes (2023)
  • Author(s): Kaisa Thorell, Zilia Y. Munoz-Ramirez, Difei Wang, Santiago Sandoval-Motta, Rajiv Boscolo Agostini, Silvia Ghirotto, Roberto C. Torres, HpGP Research Network, Daniel Falush, M. Constanza Camargo & Charles S. Rabkin
  • Journal Title: Nature Communications Volume: 14 Issue: 1 Pages: 8184-8184
  • DOI: 10.1038/s41467-023-43562-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Integrated environmental, lifestyle, and epigenetic risk prediction of primary gastric neoplasia using the longitudinally monitored cohorts (2023)
  • Author(s): Usui Genki, Matsusaka Keisuke, Huang Kie Kyon, Zhu Feng, Shinozaki Tomohiro, et al.
  • Journal Title: eBioMedicine Volume: 98 Pages: 104844-104844
  • DOI: 10.1016/j.ebiom.2023.104844
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] <scp>SETD1A</scp> function in leukemia is mediated through interaction with mitotic regulators <scp>BuGZ</scp>/<scp>BUB3</scp> (2023)
  • Author(s): Perlee Sarah、Kikuchi Sota、Nakadai Tomoyoshi、Masuda Takeshi、Ohtsuki Sumio、Matsumoto Makoto、Rahmutulla Bahityar、Fukuyo Masaki、Cifani Paolo、Kentsis Alex、Roeder Robert G、Kaneda Atsushi、Hoshii Takayuki
  • Journal Title: EMBO reports Volume: 24 Issue: 10 Pages: 1-19
  • DOI: 10.15252/embr.202357108
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Association of frequent hypermethylation with high grade histological subtype in lung adenocarcinoma (2023)
  • Author(s): Ito Yuki、Usui Genki、Seki Motoaki、Fukuyo Masaki、Matsusaka Keisuke、Hoshii Takayuki、Sata Yuki、Morimoto Junichi、Hata Atsushi、Nakajima Takahiro、Rahmutulla Bahityar、Kaiho Taisuke、Inage Terunaga、Tanaka Kazuhisa、Sakairi Yuichi、Suzuki Hidemi、Yoshino Ichiro、Kaneda Atsushi
  • Journal Title: Cancer Science Volume: Online Issue: 7 Pages: 3003-3013
  • DOI: 10.1111/cas.15817
  • Peer Reviewed / Open Access
• [Journal Article] Genetic knockdown of genes that are obscure, conserved and essential using CRISPR interference methods in the fission yeast <i>S. pombe</i> (2023)
  • Author(s): Ishikawa Ken、Soejima Saeko、Saitoh Shigeaki
  • Journal Title: Journal of Cell Science Volume: 136 Issue: 9
  • DOI: 10.1242/jcs.261186
  • Peer Reviewed / Open Access
• [Journal Article] Transcriptional Regulation Technology for Gene Perturbation in Fission Yeast (2023)
  • Author(s): Ishikawa Ken、Saitoh Shigeaki
  • Journal Title: Biomolecules Volume: 13 Issue: 4 Pages: 716-716
  • DOI: 10.3390/biom13040716
  • Peer Reviewed / Open Access
• [Journal Article] Base-excision restriction enzymes: expanding the world of epigenetic immune systems (2023)
  • Author(s): Kojima Kenji K、Kobayashi Ichizo
  • Journal Title: DNA Research Volume: 30 Issue: 4
  • DOI: 10.1093/dnares/dsad009
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Tumorigenic activation around <scp>HPV</scp> integrated sites in head and neck squamous cell carcinoma (2023)
  • Author(s): Mima Masato、Okabe Atsushi、Hoshii Takayuki、Nakagawa Takuya、Kurokawa Tomoya、Kondo Satoru、Mizokami Harue、Fukuyo Masaki、Fujiki Ryoji、Rahmutulla Bahityar、Yoshizaki Tomokazu、Hanazawa Toyoyuki、Misawa Kiyoshi、Kaneda Atsushi
  • Journal Title: International Journal of Cancer Volume: 152 Issue: 9 Pages: 1847-1862
  • DOI: 10.1002/ijc.34439
  • Peer Reviewed / Open Access
• [Journal Article] Anti-proliferating and apoptosis-inducing activity of chemical compound FTI-6D in association with p53 in human cancer cell lines (2023)
  • Author(s): Inoue Takato、Matsuda Kazuaki、Matsusaka Keisuke、Nakajima Masaya、Takeno Yukari、Miyazaki Toko、Shintaku Takahiko、Yoda Natsumi、Saito Takahiko、Ikeda Eriko、Mano Yasunobu、Shinohara Kenichi、Rahmutulla Bahityar、Fukuyo Masaki、Kita Kazuko、Nemoto Tetsuhiro、Kaneda Atsushi
  • Journal Title: Chemico-Biological Interactions Volume: 369 Pages: 110257-110257
  • DOI: 10.1016/j.cbi.2022.110257
  • Peer Reviewed
• [Journal Article] SETD1A regulates transcriptional pause release of heme biosynthesis genes in leukemia (2022)
  • Author(s): Hoshii Takayuki、Perlee Sarah、Kikuchi Sota、Rahmutulla Bahityar、Fukuyo Masaki、Masuda Takeshi、Ohtsuki Sumio、Soga Tomoyoshi、Nabet Behnam、Kaneda Atsushi
  • Journal Title: Cell Reports Volume: 41 Issue: 9 Pages: 111727-111727
  • DOI: 10.1016/j.celrep.2022.111727
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Repression of DERL3 via DNA methylation by Epstein-Barr virus latent membrane protein 1 in nasopharyngeal carcinoma (2022)
  • Author(s): Kondo S, Okabe A, Nakagawa T, Matsusaka K, Fukuyo M, Rahmutulla B, Dochi H, Mizokami H, Kitagawa Y, Kurokawa T, Mima M, Endo K, Sugimoto H, Wakisaka N, Misawa K, Yoshizaki T, Kaneda A.
  • Journal Title: Biochimica et Biophysica Acta - Molecular Basis of Disease Volume: 1869(2) Issue: 2 Pages: 166598-166598
  • DOI: 10.1016/j.bbadis.2022.166598
  • Peer Reviewed / Open Access
• [Journal Article] Proteogenomic landscape and clinical characterization of GH-producing pituitary adenomas/somatotroph pituitary neuroendocrine tumors (2022)
  • Author(s): Yamato A,Nagano H,Gao Y,Matsuda T,Hashimoto N,Nakayama A,Yamagata K,Yokoyama M,Gong Y,Shi X,Zhahara N,Kono T,Taki Y,Furuki N,Nishimura M,Horiguchi K,Iwadate Y,Fukuyo M,Rahmutulla B,Kaneda A,Hasegawa Y,Kawashima Y,Ohara O,Ishikawa T,Kawakami E,Nakamura Y,Inoshita N,Yamada S,Fukuhara N,Nishioka H,Tanaka T
  • Journal Title: Communications Biology Volume: 5 Issue: 1 Pages: 1304-1304
  • DOI: 10.1038/s42003-022-04272-1
  • Peer Reviewed / Open Access
• [Journal Article] Adenocarcinoma of the stomach and esophagogastric junction with low DNA methylation show poor prognoses (2022)
  • Author(s): Urabe Masayuki、Matsusaka Keisuke、Ushiku Tetsuo、Fukuyo Masaki、Rahmutulla Bahityar、Yamashita Hiroharu、Seto Yasuyuki、Fukayama Masashi、Kaneda Atsushi
  • Journal Title: Gastric Cancer Volume: 26 Issue: 1 Pages: 95-107
  • DOI: 10.1007/s10120-022-01344-3
  • Peer Reviewed / Open Access
• [Journal Article] Single-cell immunoprofiling after immunotherapy for allergic rhinitis reveals functional suppression of pathogenic TH2 cells and clonal conversion (2022)
  • Author(s): Iinuma Tomohisa、Kiuchi Masahiro、Hirahara Kiyoshi、Kurita Junya、Kokubo Kota、Yagyu Hiroyuki、Yoneda Riyo、Arai Tomoyuki、Sonobe Yuri、Fukuyo Masaki、Kaneda Atsushi、Yonekura Syuji、Nakayama Toshinori、Okamoto Yoshitaka、Hanazawa Toyoyuki
  • Journal Title: Journal of Allergy and Clinical Immunology Volume: 150 Issue: 4 Pages: 850-860.e5
  • DOI: 10.1016/j.jaci.2022.06.024
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characteristic DNA methylation profiles of chorionic villi in recurrent miscarriage (2022)
  • Author(s): Matsumoto Y, Shinjo K, Mase S, Fukuyo M, Aoki K, Ozawa F, Yoshihara H, Goto S, Kitaori T, Ozaki Y, Takahashi S, Kaneda A, Sugiura-Ogasawara M, Kondo Y.
  • Journal Title: Scientific Reports Volume: 12 Issue: 1 Pages: 11673-11673
  • DOI: 10.1038/s41598-022-15656-y
  • Peer Reviewed / Open Access
• [Journal Article] Association of tumors having <scp>Epstein?Barr</scp> virus in surrounding lymphocytes with poor prognosis (2022)
  • Author(s): Yogi Norikazu、Usui Genki、Matsusaka Keisuke、Fukuyo Masaki、Fujiki Ryoji、Seki Motoaki、Takano Shigetsugu、Abe Hiroyuki、Morikawa Teppei、Ushiku Tetsuo、Ohtsuka Masayuki、Kaneda Atsushi
  • Journal Title: Cancer Medicine Volume: 12 Issue: 2 Pages: 1122-1136
  • DOI: 10.1002/cam4.4967
  • Peer Reviewed
• [Journal Article] Estrogen induces the expression of <scp>EBV</scp> lytic protein <scp>ZEBRA</scp> , a?marker of poor prognosis in nasopharyngeal carcinoma (2022)
  • Author(s): Dochi H、Kondo S、Murata T、Fukuyo M、Nanbo A、Wakae K、Jiang P、Hamabe T、Tanaka M、Nishiuchi T、Mizokami H、Moriyama M、Kobayashi E、Hirai N、Komori T、Ueno T、Nakanishi Y、Hatano M、Endo K、Sugimoto H、Wakisaka N、Juang H、Muramatsu M、Kaneda A、Yoshizaki T
  • Journal Title: Cancer Science Volume: 113 Issue: 8 Pages: 2862-2877
  • DOI: 10.1111/cas.15440
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cinobufagin inhibits proliferation of acute myeloid leukaemia cells by repressing c-Myc pathway-associated genes (2022)
  • Author(s): Hirasaki Yoshiro、Okabe Atsushi、Fukuyo Masaki、Rahmutulla Bahityar、Mano Yasunobu、Seki Motoaki、Hoshii Takayuki、Namiki Takao、Kaneda Atsushi
  • Journal Title: Chemico-Biological Interactions Volume: 360 Pages: 109936-109936
  • DOI: 10.1016/j.cbi.2022.109936
  • Peer Reviewed
• [Journal Article] Genomic differentiation within East Asian Helicobacter pylori (2022)
  • Author(s): You Yuanhai、Thorell Kaisa、He Lihua、Yahara Koji、Yamaoka Yoshio、Cha Jeong-Heon、Murakami Kazunari、Katsura Yukako、Kobayashi Ichizo、Falush Daniel、Zhang Jianzhong、TEAMHp
  • Journal Title: Microbial Genomics Volume: 8 Issue: 2 Pages: 1-14
  • DOI: 10.1099/mgen.0.000676
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] The virus-host genomic interactions drive heterochromatin disruption and oncogenic activation of enhancers. (2023)
  • Author(s): Atsushi Okabe, Takahiro Fujii, Harue Mizokami, Keisuke Matsusaka, Masaki Fukuyo, Bahityar Rahmutulla, Patrick Tan, Hironori Yoshiyama, Atsushi Kaneda
  • Organizer: 第16回エピジェネティクス研究会年会
• [Presentation] Identification of Aberrant Enhancer Transcriptional Regulatory Network in Gastric Cancer by Genome-wide HiChIP Analysis. (2023)
  • Author(s): Tianhui Zhu, Atsushi Okabe, Takayuki Hoshii, Ryoji Fujiki, Motoaki Seki, Meng Ning, Bahityar Rahmutulla, Masaki Fukuyo, Atsushi Kaneda
  • Organizer: 第16回エピジェネティクス研究会年会
• [Presentation] Functional Analysis of H3K4 methyltransferase promoting Gastric Cancer progression. (2023)
  • Author(s): Meng Ning, Takayuki Hoshii, Kanako Hayashi, Shintaro Izumi, Tianhui Zhu, Makoto Matsumoto, Bahityar Rahmutulla, Masaki Fukuyo, Atsushi Kaneda
  • Organizer: 第16回エピジェネティクス研究会年会
• [Presentation] Compartment changes and epigenetic activation of cancer-related genes in castration resistant prostate cancer. (2023)
  • Author(s): Sanji Kanaoka, Atsushi Okabe, Manato Kanesaka, Takayuki Hoshii, Masaki Fukuyo, Bahityar Rahmutulla, Yusuke Imamura, Shinichi Sakamoto, Tomohiko Ichikawa, Atsushi Kaneda
  • Organizer: 第16回エピジェネティクス研究会年会
• [Presentation] HBV感染肝細胞におけるエピゲノムおよびクロマチン相互作用の解析. (2023)
  • Author(s): 込山 大智、岡部 篤史、葛西 宏威、福世 真樹、バハテヤリ・ラヒムトラ、渡士 幸一、森石 恆司、金田 篤志
  • Organizer: 第16回エピジェネティクス研究会年会
• [Presentation] Integrated analysis reveals epigenetic abnormalities and identifies transcription factors important for survival and proliferation in hepatoblastoma. (2023)
  • Author(s): 工藤渉、星居孝之、関元昭、岡部篤史、福世真樹、バハテヤリラヒムトラ、菱木知郎、金田篤志
  • Organizer: 第16回エピジェネティクス研究会年会
• [Presentation] Risk stratification of primary GC by integrating clinicopathological factors and DNA methylation in the gastric mucosa. (2023)
  • Author(s): Genki Usui, Tomoka Okada, Mizuki Minami, Masaki Fukuyo, Bahityar Rahmutulla, Motoaki Seki, Eiji Sakai, Keisuke Matsusaka, Tetsuo Ushiku, Atsushi Kaneda
  • Organizer: 第82回日本癌学会学術総会
• [Presentation] Functional Analysis of SETD1A in Promoting Gastric Cancer Cell Proliferation. (2023)
  • Author(s): Meng Ning, Takayuki Hoshii, Kanako Hayashi, Shintaro Izumi, Tianhui Zhu, Makoto Matsumoto, Bahityar Rahmutulla, Masaki Fukuyo, Atsushi Kaneda
  • Organizer: 第82回日本癌学会学術総会
• [Presentation] Integrated Genome-wide HiChIP Analysis Identified Aberrant Enhancer Transcriptional Regulatory Network in Gastric Cancer. (2023)
  • Author(s): Tianhui Zhu, Atsushi Okabe, Takayuki Hoshii, Ryoji Fujiki, Motoaki Seki, Meng Ning, Bahityar Rahmutulla, Masaki Fukuyo, Atsushi Kaneda
  • Organizer: 第82回日本癌学会学術総会
• [Presentation] 去勢抵抗性前立腺がんにおけるコンパートメント変化と癌関連遺伝子の異常活性化. (2023)
  • Author(s): 金岡尚志、岡部篤史、金坂学斗、星居孝之、福世真樹、バハテヤリラヒムトラ、今村有佑、坂本信一、市川智彦、金田篤志
  • Organizer: 第82回日本癌学会学術総会
• [Presentation] Identifying key transcription factors to survival and growth in hepatoblastoma by integrated analysis. (2023)
  • Author(s): 工藤渉、星居孝之、関元昭、岡部篤史、福世真樹、バハテヤリラヒムトラ、菱木知郎、金田篤志
  • Organizer: 第82回日本癌学会学術総会
• [Presentation] SETD1A drives proliferation of hormone receptor-positive breast cancer cells independent of its enzymatic activity. (2023)
  • Author(s): Kanako Hayashi, Takayuki Hoshii, Makoto Matsumoto, Bahityar Rahmutulla, Masaki Fukuyo, Masahiko Tanabe, Yasuyuki Seto, Atsushi Kaneda
  • Organizer: 第82回日本癌学会学術総会
• [Presentation] Integrated epigenomic analyses of aberrantly regulated enhancers and transcriptional networks in gastric cancer. (2023)
  • Author(s): Tianhui Zhu, Atsushi Okabe, Takayuki Hoshii, Ryoji Fujiki, Motoaki Seki, Meng Ning, Bahityar Rahmutulla, Masaki Fukuyo, Atsushi Kaneda
  • Organizer: Cold Spring Harbor Asia Meeting-Systems Biology of Gene Regulation and Genome Editing．
  • Int'l Joint Research
• [Presentation] 臨床因子と胃粘膜に蓄積したDNAメチル化を統合した一次胃癌のリスク層別化 (2023)
  • Author(s): 臼井源紀、松坂恵介、Kie Kyon Huang、Feng Zhu、篠崎智大、福世真樹、バハテヤリラヒムトラ、与儀憲和、岡田朋香、南瑞樹、関元昭、酒井英嗣、阿部浩幸、郡司俊秋、松橋信行、森川鉄平、牛久哲男、Khay Guan Yeoh、Patrick Tan、金田篤志
  • Organizer: 第34回日本消化器癌発生学会総会
• [Presentation] 肝芽腫の生存と増殖に重要な転写因子の同定. (2023)
  • Author(s): 工藤渉、星居孝之、関元昭、岡部篤史、福世真樹、バハテヤリラヒムトラ、菱木知郎、金田篤志
  • Organizer: 第34回日本消化器癌発生学会総会
• [Presentation] 癌ウイルス感染が誘導するクロマチン構造異常 (2023)
  • Author(s): 岡部篤史, 藤井貴大、溝上晴恵、松坂恵介、福世真樹、Bhityar Rahmutulla、Patrick Tan、金田篤志
  • Organizer: 第34回日本消化器癌発生学会総会
• [Presentation] Chromatin structural alteration induced by oncovirus infection aberrantly activate transcriptional regulation (2023)
  • Author(s): Atsushi Okabe, Takahiro Fujii, Harue Mizokami, Keisuke Matsusaka, Masaki Fukuyo, Bahityar Rahmutulla, Patrick Tan, Hironori Yoshiyama, Atsushi Kaneda
  • Organizer: 第46回日本分子生物学会年会
• [Presentation] The Roles of SETD1A in Supporting Gastric Cancer Cell Survival. (2023)
  • Author(s): Meng Ning, Takayuki Hoshii, Kanako Hayashi, Shintaro Izumi, Tianhui Zhu, Makoto Matsumoto, Bahityar Rahmutulla, Masaki Fukuyo, Atsushi Kaneda
  • Organizer: 第46回日本分子生物学会年会
• [Presentation] Integrated Epigenomic Analyses of Aberrantly Regulated Enhancers and Transcriptional Networks in Gastric Cancer. (2023)
  • Author(s): Tianhui Zhu, Atsushi Okabe, Takayuki Hoshii, Ryoji Fujiki, Motoaki Seki, Meng Ning, Bahityar Rahmutulla, Masaki Fukuyo, Atsushi Kaneda
  • Organizer: 第46回日本分子生物学会年会
• [Presentation] HBV感染肝細胞におけるエピゲノムおよびクロマチン相互作用の解析. (2023)
  • Author(s): 込山 大智、岡部 篤史、葛西 宏威、福世 真樹、バハテヤリ・ラヒムトラ、渡士 幸一、森石 恆司、金田 篤志
  • Organizer: 第46回日本分子生物学会年会
• [Presentation] Pinpoint adaptive evolution emerging from 100s of H. pylori genome comparison (2023)
  • Author(s): Ichizo Kobayashi
  • Organizer: 第29 回日本ヘリコバクター学会学術集会
• [Presentation] Epigenome microevolution associated with DNA methyltransferases’ sequence-specificity alteration in H. pylori. (2022)
  • Author(s): Masaki Fukuyo
  • Organizer: The 21st International Workshop on Campylobacter, Helicobacter and Related Organisms.
  • Int'l Joint Research / Invited
• [Presentation] Analysis of chromatin structural aberrations in Epstein-Barr virus (+) Burkitt lymphoma． (2022)
  • Author(s): Atsushi Okabe, Takahiro Fujii, Masaki Fukuyo, Bahityar Rahmutulla, Hironori Yoshiyama, Patrick Tan, Atsushi Kaneda
  • Organizer: 20th International Symposium on Epstein-Barr virus and Associated Diseases
  • Int'l Joint Research
• [Presentation] DNAメチル化特性を利用した胃癌の分子多様性に対する戦略． (2022)
  • Author(s): 金田篤志、岡部篤史、臼井源紀、松坂恵介、福世真樹、深山正久
  • Organizer: 第33回日本消化器癌発生学会総会
• [Presentation] DNA メチル化酵素の配列特異性変換によるピロリ菌エピゲノムのミクロ進化 (2022)
  • Author(s): 福世真樹、米澤 英雄、今野 武津子、小林 一三
  • Organizer: 第28回日本ヘリコバクター学会学術集会
• [Presentation] Analyses of gastric cancer risk and DNA methylation accumulation in the gastric mucosa using the large check-up cohort (2022)
  • Author(s): 臼井源紀、松坂恵介、篠崎智大、福世真樹、バハテヤリラヒムトラ、阿部浩幸、郡司俊秋、松橋信行、森川鉄平、牛久哲男、金田篤志
  • Organizer: 第15回日本エピジェネティクス研究会
• [Presentation] 大規模コホートを用いた胃発癌リスク因子の探索および胃粘膜に蓄積したDNAメチル化の解析 (2022)
  • Author(s): 臼井源紀、松坂恵介、福世真樹、バハテヤリラヒムトラ、岡田朋香、与儀憲和、関元昭、酒井英嗣、牛久哲男、金田篤志
  • Organizer: 第81回日本癌学会総会
• [Presentation] 環境因子およびDNAメチル化蓄積による一次胃癌の発症リスク評価 (2022)
  • Author(s): 臼井源紀、Kie Kyon Huang、松坂恵介、篠崎智大、福世真樹、バハテヤリラヒムトラ、岡田朋香、酒井英嗣、阿部浩幸、郡司俊秋、松橋信行、森川鉄平、牛久哲男、Patrick Tan、金田篤志
  • Organizer: 第33回日本消化器癌発生学会総会
• [Presentation] Epigenetic activation of regions surrounding HPV integrated sites contributes to tumorigenesis of HNSCC (2022)
  • Author(s): Masato Mima, Atsushi Okabe, Takuya Nakagawa, Tomoya Kurokawa, Satoru Kondo, Harue Mizokami, Masaki Fukuyo, Bhityar R. Nawai, Takayuki Hoshii, Kiyoshi Misawa, Atsushi Kaneda
  • Organizer: 第81回日本癌学会学術総会
• [Presentation] Analysis of chromatin structural aberrations in Epstein-Barr virus (+) Burkitt lymphoma (2022)
  • Author(s): Atsushi Okabe, Takahiro Fjii, Keisuke Matsusaka, Masaki Fukuyo, Bahityar Rahmutulla, Patrick Tan, Atsushi Kaneda
  • Organizer: 第15回日本エピジェネティクス研究会
• [Presentation] Analysis of chromatin structural aberrations induced by oncovirus infection (2022)
  • Author(s): Atsushi Okabe, Takahiro Fujii, Masaki Fukuyo, Bahityar Rahmutulla, Hironori Yoshiyama, Patrick Tan, Atsushi Kaneda
  • Organizer: 第81回日本癌学会学術総会
• [Presentation] Rewiring of heterochromatin by Epstein-Barr virus genome in nasopharyngeal carcinoma (2022)
  • Author(s): Harue Mizokami, Atsushi Okabe, Masato Mima, Satoru Kondo, Masaki Fukuyo, Bahityar Rahmutulla, Tomokazu Yoshizaki, Atsushi Kaneda
  • Organizer: 第81回日本癌学会学術総会
• [Presentation] 分裂酵母とヒト間で進化的に保存された未解析必 須遺伝子のCRISPRiによるノックダウン (2022)
  • Author(s): 石川健、副島朗子、齋藤成昭
  • Organizer: 日本遺伝学会 第94回大会
• [Presentation] CRISPRiを用いた分裂酵母の全必須遺伝子ノック ダウンライブラリ構築への取り組み (2022)
  • Author(s): 石川健、副島朗子、齋藤成昭
  • Organizer: 第45回分子生物学会年会