| Project/Area Number |
22K07320
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 51010:Basic brain sciences-related
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| Research Institution | University of Toyama |
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Principal Investigator |
YOUNG MEGAN 富山大学, 学術研究部医学系, 特命助教 (50930830)
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| Co-Investigator(Kenkyū-buntansha) |
田村 了以 富山大学, 学術研究部医学系, 教授 (60227296)
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| Project Period (FY) |
2022-04-01 – 2025-03-31
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| Project Status |
Granted (Fiscal Year 2023)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2024: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2023: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2022: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | hippocampus / non-human primate / monkey / CA1 / place field / freely-moving / neuronal recording / primate / electrophysiology |
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| Outline of Research at the Start |
The firing patterns of spatially-selective neurons in the hippocampus play a critical role in spatial navigation. Using a 3D maze, a wireless data logger, and deep learning-powered pose estimation, we will compare the horizontal and vertical response properties of place cells in the hippocampus.
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| Outline of Annual Research Achievements |
Our purpose in this study is to record neuronal activity from the hippocampus of freely-moving monkeys in a three-dimensional environment to evaluate the relationship between movement direction and place cell responsiveness. We have trained a monkey to move within this three-dimensional environment by rotating clockwise or counterclockwise, and continue development of a primate behavior monitoring system using AI. This year, we used evoked potentials to accurately map the location of the hippocampus and successfully recorded a number of hippocampal neurons stably with good signal within our three-dimensional environment under awake conditions using telemetry.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Behavioral monitoring using a camera system with TTL synchronization to the neuronal recording via telemetry has been established and works reliably. The monkey has been well trained to navigate the 3D environment in both the clockwise and counter clockwise directions. Mapping to locate the hippocampus with high fidelity has been performed and has enabled precise targeting of the microdrive-equipped tetrodes. Neuronal recording via chronically implanted tetrodes has also been proceeding smoothly, with stable signal over long time courses, including during behavior as the animal freely navigates the 3D environment.
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| Strategy for Future Research Activity |
We will continue to record signals from more hippocampal neurons as the monkey navigates the 3D environment, with simultaneous synchronized video tracking using our camera system. We will proceed with analyzing the recorded signals with cluster cutting and analysis of the spike data using the animal positions provided by our behavioral tracking system.
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