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Development of a novel co-culture model of NH4-tolerant propionate-oxidizing bacteria and methane-producing archaea for the recovery of CH4 under high NH4+

Research Project

Project/Area Number 22K12428
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 64020:Environmental load reduction and remediation-related
Research InstitutionOsaka University

Principal Investigator

Luong Van・Duc  大阪大学, 大学院工学研究科, 特任研究員(常勤) (60815174)

Co-Investigator(Kenkyū-buntansha) 井上 大介  大阪大学, 大学院工学研究科, 准教授 (70448091)
Project Period (FY) 2022-04-01 – 2025-03-31
Project Status Granted (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2024: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2023: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsanaerobic digestion / propionate oxidizers / ammonium / high ammonium / propionate / carbon recovery / methane recovery / propionate oxidizer / methane producer
Outline of Research at the Start

Anaerobic digestion (AD) is a sustainable technology for CH4 recovery from wastewater as energy. Yet, the application of AD under high NH4+ remains a big challenge because high NH4+ inhibits the activity of propionate-oxidizing bacteria (POB), which results in low production of CH4. We will enrich and couple NH4-tolerant POB with methane-producing archaea for the enhanced CH4 recovery under high NH4+.

Outline of Annual Research Achievements

A key factor for the enrichment of propionate-oxidizers has been explored. Also, the enriched culture showed a good performance in increasing the amount and rate of CH4 production when testing with different kinds of synthetic wastewater under high ammonium concentrations.

Current Status of Research Progress
Current Status of Research Progress

3: Progress in research has been slightly delayed.

Reason

Though the high effectiveness of the enriched culture has been shown to relieve the inhibition of ammonium in simulated wastewater, a large amount of biomass was used in this test. Therefore, an extended period is needed to obtain a desirable biomass for subsequent tests.

Strategy for Future Research Activity

An optimal culture of propionate oxidizing microorganisms and their characteristics will be characterized for smooth production of CH4 under high ammonium and propionate concentrations.

Report

(2 results)
  • 2023 Research-status Report
  • 2022 Research-status Report
  • Research Products

    (6 results)

All 2023 2022

All Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results) Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Bioaugmentation with marine sediment-derived microbial consortia in mesophilic anaerobic digestion for enhancing methane production under ammonium or salinity stress2023

    • Author(s)
      Luong Van Duc, Shintaro Nagao, Mohammad Mojarrad, Yuta Miyagawa, Zi-Yan Li, Daisuke Inoue, Takahisa Tajima, Michihiko Ike
    • Journal Title

      Bioresource Technology

      Volume: 376 Pages: 128853-128853

    • DOI

      10.1016/j.biortech.2023.128853

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Identification of key steps and associated microbial populations for efficient anaerobic digestion under high ammonium or salinity conditions2022

    • Author(s)
      Luong Van Duc, Yuta Miyagawa, Daisuke Inoue, Michihiko Ike
    • Journal Title

      Bioresource Technology

      Volume: 360 Pages: 127571-127571

    • DOI

      10.1016/j.biortech.2022.127571

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Aggregation and Dispersion Behaviours of Riverine Trace Metals (Fe, Al, V, Mn, Ni, and Zn) and Organic Matter in Freshwater and Estuarine Conditions: A case study in Shira and Midori Rivers, Kumamoto, Japan2022

    • Author(s)
      Ito Hiroaki、Tsurumaki Shinya、Hama Takehide、Ishida Kei、Watanabe Tsugihiro、van Duc Luong、Kawagoshi Yasunori
    • Journal Title

      Aquatic Geochemistry

      Volume: 29 Issue: 1 Pages: 1-23

    • DOI

      10.1007/s10498-022-09408-7

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 高アンモニア性窒素・塩分濃度下における 嫌気性消化の阻害緩和に資する プロピオン酸酸化細菌群集の集積及び特徴づけ2022

    • Author(s)
      Yuta Miyagawa
    • Organizer
      日本水処理生物学会第58回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] バイオオーグメンテーションによる嫌気性消化への高濃度塩分阻害の緩和2022

    • Author(s)
      Shintaro Nagao
    • Organizer
      第22回環境技術学会年次大会
    • Related Report
      2022 Research-status Report
  • [Presentation] BIOAUGMENTATION WITH MARINE SEDIMENT-DERIVED MICROBIAL CONSORTIA TO ENHANCE ANAEROBIC DIGESTION UNDER HIGH AMMONIUM OR SALINITY2022

    • Author(s)
      Luong Van Duc
    • Organizer
      Grand Renewable Energy 2022 International Conference(GRE2022)
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research

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Published: 2022-04-19   Modified: 2024-12-25  

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