Development of a novel co-culture model of NH4-tolerant propionate-oxidizing bacteria and methane-producing archaea for the recovery of CH4 under high NH4+
Project/Area Number |
22K12428
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 64020:Environmental load reduction and remediation-related
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Research Institution | Osaka University |
Principal Investigator |
Luong Van・Duc 大阪大学, 大学院工学研究科, 特任研究員(常勤) (60815174)
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Co-Investigator(Kenkyū-buntansha) |
井上 大介 大阪大学, 大学院工学研究科, 准教授 (70448091)
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Project Period (FY) |
2022-04-01 – 2025-03-31
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Project Status |
Granted (Fiscal Year 2022)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2024: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2023: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | anaerobic digestion / high ammonium / propionate / propionate oxidizers / carbon recovery / methane recovery / propionate oxidizer / methane producer |
Outline of Research at the Start |
Anaerobic digestion (AD) is a sustainable technology for CH4 recovery from wastewater as energy. Yet, the application of AD under high NH4+ remains a big challenge because high NH4+ inhibits the activity of propionate-oxidizing bacteria (POB), which results in low production of CH4. We will enrich and couple NH4-tolerant POB with methane-producing archaea for the enhanced CH4 recovery under high NH4+.
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Outline of Annual Research Achievements |
We have successfully enriched NH4-tolerant propionate-oxidizing bacteria by batch transfers. The enrichment culture can oxidize propionate under 5 gNH4-N/L. Next-generation sequencing showed the dominance of propionate-oxidizing bacteria, including Pelotomaculum and Cryptanaerobacter. In addition, the increase of hydrogenotrophic methanogens Methanoculleus was also observed, indicating the syntrophic relationship between NH4-tolerant propionate-oxidizing bacteria and hydrogenotrophic methanogens.
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Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
Because the activity of NH4-tolerant propionate-oxidizing bacteria is unstable after several transfers. Therefore, the enrichment period is extended more than expected to optimize the enrichment culture and increase biomass. The extended period is necessary to maintain the activity of propionate-oxidizing bacteria before proceeding to the following steps.
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Strategy for Future Research Activity |
Higher concentrations of propionate and NH4+ will be introduced to test the tolerance of the enriched culture. Next, different combinations of propionate-oxidizing bacteria and archaea will be considered for stable and effective production of CH4 under high NH4+ concentrations.
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Report
(1 results)
Research Products
(6 results)