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Development of online algae counting technique for avoiding musty odor in drinking water

Research Project

Project/Area Number 22K14352
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 22060:Environmental systems for civil engineering-related
Research InstitutionNagasaki University

Principal Investigator

ブアバー サンドリンルーシーパスカル  長崎大学, 工学研究科, 特任研究員 (80896704)

Project Period (FY) 2022-04-01 – 2025-03-31
Project Status Granted (Fiscal Year 2023)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2024: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2023: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2022: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsdrinking water / algae / odor / monitoring
Outline of Research at the Start

The unpredictable occurrence of musty-odor substances in drinking water is a major challenge for many drinking water utilities. This research aims to develop an online monitoring system based on fluorescence microscopy that will continuously counts various types of odor-producing algae 24/7.

Outline of Annual Research Achievements

The properties of various algae in a surface water (Biwa Lake) was assessed using an automated fluorescence microscope. As a result, the concentrations of Pseudanabaena sp. were auto-counted and the accuracy of the analysis was acceptable. However, due to low frequency of the odor-producing algae occurrence, the chances of the auto-counting technique validation did not happen frequently. It was found that the frequency of odor-causing algae occurrence plays an important role in the method validation. In addition to Pseudanabaena sp., the criteria for automatically counting other algae causing problems for the drinking water treatment plant has also been established. Further, lenses to capture clear image for improving the accuracy of the auto-counting method were assessed and the best lens has been identified and incorporated in the system.

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

The criteria (dimensions, shape, fluorescence intensity) for automatically identifying odor-producing algae other than Pseudanabaena sp. has been assessed and determined. To capture clear image for accurately counting the target algae, a new lens has been selected and incorporated into the auto-counting system.

Strategy for Future Research Activity

To judge the practicality of the developed online monitoring system, it is necessary to establish the accuracy of the automatically counted concentrations and the system stability for long-term operation. Therefore, on-site monitoring test will be conducted for the period of potential odor occurrence.

Report

(2 results)
  • 2023 Research-status Report
  • 2022 Research-status Report
  • Research Products

    (2 results)

All 2023

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (1 results)

  • [Journal Article] The rapid counting method for 2-MIB-producing cyanobacteria (Pseudanabaena sp.) using fluorescence detection of phycocyanin pigments in algal cells2023

    • Author(s)
      Boivin Sandrine、Hasegawa Eri、Yamaguchi Dabide、Fujioka Takahiro
    • Journal Title

      Environmental Science: Water Research & Technology

      Volume: 9 Issue: 10 Pages: 2561-2568

    • DOI

      10.1039/d3ew00282a

    • Related Report
      2023 Research-status Report
    • Peer Reviewed
  • [Presentation] Monitoring of 2-MIB Producing Cyanobacteria (Pseudanabaena sp.) in Drinking Water Sources by Automated Cell Imaging Using Phycocyanin Fluorescence2023

    • Author(s)
      Sandrine Boivin; Takahiro Fujioka; Eri Hasegawa; Dabide Yamaguchi
    • Organizer
      第57回日本水環境学会年会
    • Related Report
      2022 Research-status Report

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Published: 2022-04-19   Modified: 2024-12-25  

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