| Project/Area Number |
22K14711
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 34020:Analytical chemistry-related
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| Research Institution | National Institute for Materials Science |
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Principal Investigator |
GANGANBOINA Akhilesh Babu 国立研究開発法人物質・材料研究機構, 若手国際研究センター, ICYSリサーチフェロー (90937252)
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2022: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | Photoelecrtochemical / Biosensor / Nanomaterial / water oxidation / Encapsulation / Virus / Visible light / Self powered / Virus sensor / Photoelectrochemical / self powered / Nanohetrostructure |
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| Outline of Research at the Start |
In this project, we will focus on the developing a cost-effective self-powered photoelectrochemical virus biosensing platform for the rapid point of care diagnosis. A completely new attempt for the visible-light-driven self-powered device based on Co3O4-CdSe-CdS-Pt NPs will be developed for real-time detection. The detection signal is majorly based on the photocurrent response generated by Co3O4-CdSe-CdS-Pt upon excitation with visible light. The whole project's success can make a self-powered visible-light-driven device for a rapid detection of viruses.
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| Outline of Final Research Achievements |
In the absence of therapeutic agents, early virus detection is crucial to prevent outbreaks and control pandemics. We developed a self-powered photoelectrochemical (PEC) biosensor for ultrasensitive detection of hepatitis E virus (HEV), HEV-like particles (HEV-LPs), and SARS-CoV-2 spike protein in complex lysate solutions. This biosensor uses novel electrocatalysts CdSe-Co3O4@TiO2 and Co3O4-CdSe-CdS-Pt, generating a photocurrent response under visible light. Encapsulation within a polymer shell amplifies the detection signal. Enhanced photocurrent response is due to CdSe quantum dots' sensitization and strong light absorption, coupled with Co3O4's stability. The biosensor shows a linear relationship between photocurrent output and virus concentration (10 fg/mL to 10 ng/mL), offering high sensitivity, specificity, and stability. This development shows significant potential for creating affordable, portable biosensing devices
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| Academic Significance and Societal Importance of the Research Achievements |
この研究は、ウイルス検出技術の大きな進歩を表しています。新しい光電気触媒を開発することで、感度と光電流応答が大幅に向上しました。このデバイスは、10 fg/mLという非常に低い濃度のE型肝炎ウイルスとSARS-CoV-2スパイクタンパク質を検出できます。手頃な価格で持ち運び可能な当社のバイオセンシングデバイスは、現場での迅速なウイルス検出を提供します。これは、特にリソースが限られた環境でのタイムリーな公衆衛生対応に不可欠です。この技術は、感染率を下げ、医療システムへの負担を軽減し、最終的には人命を救うのに役立ち、世界の健康に大きなプラスの影響をもたらします。
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