| Project/Area Number |
22K14805
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 38010:Plant nutrition and soil science-related
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| Research Institution | Okayama University |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2022: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | アンモニウム / イネ / アンモニウム輸送体 / 極性局在 / 小胞体蓄積 / リン酸化 / 翻訳後制御 |
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| Outline of Research at the Start |
イネはアンモニウムを好んで利用するため、効率的なアンモニウム利用機構を有すると考えられる。しかし、イネ独自のアンモニウム利用の分子機構はこれまで見出されていない。申請者は、3種類のアンモニウム輸送体 (AMT1) が、外皮細胞の遠心側へ偏在し、アンモニウムに応じて小胞体に蓄積するというイネに特有の現象を発見した。これらの現象は、イネの効率的なアンモニウム利用に貢献している可能性があるが、その生理的意義は分かっていない。本申請では、AMT1の部分置換によって偏在性や小胞体蓄積性を失ったAMT1を作出し、正常なAMT1との比較から、イネAMT1に特徴的な翻訳後制御の生理的意義を明らかにする。
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| Outline of Final Research Achievements |
Ammonium is a major nitrogen source for paddy-growing rice, and three ammonium transporter1 (AMT1) members are responsible for its uptake. In rice, AMT1 members polarly localize at the distal side of the plasma membrane, and they accumulate in ER after ammonium supply. However, the physiological significance and mechanisms underlying these posttranslational regulations are poorly understood.
Here, we analyzed the localization of various AMT1;1 deletion variants and point-mutated AMT1;2 variants to investigate the region involved in their polar localization and ER accumulation. Overall, this analysis indicates that 1) phosphorylations at the C-terminal cytosolic region of AMT1;2 was not required for its polar localization, 2) the 13 amino acid region of AMT1;1 C-terminus was involved in its membrane trafficking. Furthermore, by co-immunoprecipitation, we identify several interacting proteins of AMT1;1 and AMT1;2 as potential regulators of their posttranslational regulations.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、AMT1のイネに特徴的な翻訳後制御機構の解明に向けての端緒をつかむことができた。今後、イネの窒素利用効率の向上に資する発見につながると期待される。
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