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Structural insight into the regulation of genes controlling the legume nodule for improving agricultural traits in crops

Research Project

Project/Area Number 22K14824
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 38030:Applied biochemistry-related
Research InstitutionUniversity of Tsukuba

Principal Investigator

Nosaki Shohei  筑波大学, 生命環境系, 助教 (20850910)

Project Period (FY) 2022-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2022: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords転写因子 / 構造生物学 / マメ科植物 / 根粒形成 / 窒素栄養応答 / 遺伝子制御 / 相互作用解析 / 窒素吸収 / X線結晶構造解析
Outline of Research at the Start

根粒菌と共生窒素固定を行うマメ科植物は、窒素栄養が豊富な土壌にて根粒共生を自ら抑える機構をもつが故に、農業上において望ましい “根粒共生と窒素肥料による窒素栄養の獲得の両立” が困難となっている。本研究では、栄養豊富な土壌環境下でも根粒共生できるマメ科植物の開発を見据え、マスター転写因子が司る根粒形成遺伝子スイッチング機構を物理化学的な側面から解明することに取り組む。本研究での成果は、植物-微生物共生の研究分野に新規軸を打ち出すとともに、ダイズなどの農業上重要なマメ科植物の中で眠る潜在能力を最大限に引き出すための基盤知見にもなると期待される。

Outline of Final Research Achievements

Because legumes have a mechanism to suppress root nodule symbiosis by themselves in soils rich in nitrogen nutrients, it has been difficult to achieve the desired agricultural traits of both root nodule symbiosis and the acquisition of nitrogen nutrients through fertilizers. In this study, we aimed to develop leguminous plants that can coexist with root nodules even in nutrient-rich soil environments, and attempted to elucidate the mechanism by which nodule formation is controlled by the key transcription factors from a physical and chemical perspective. During the two-year research period, we deepened our understanding of the transcriptional control system that underlies the basis of nodule formation control and nitrogen nutrient response. In addition, new findings were also obtained regarding the molecular transition between the transcription factor NIN, which controls nodule symbiosis, and the nitrogen nutrient response regulator NLP during the acquisition of nodule symbiosis.

Academic Significance and Societal Importance of the Research Achievements

本研究は、マメ科植物を代表とする根粒共生植物の根粒形成ならびに窒素栄養応答を制御する転写制御システムの理解を飛躍的に高めたといえる。また、根粒共生植物を制御する転写因子が窒素栄養応答の制御因子からどのような分子変遷を辿り根粒共生獲得を駆動したのかという点について新たな知見をもたらすことができた。将来的にダイズなどの農業上重要なマメ科植物の中で眠る潜在能力を最大限に引き出すための基盤知見になると期待される。

Report

(3 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • Research Products

    (20 results)

All 2024 2023 2022 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (14 results) (of which Int'l Joint Research: 6 results,  Invited: 2 results) Remarks (2 results)

  • [Journal Article] BPG4 regulates chloroplast development and homeostasis by suppressing GLK transcription factors and involving light and brassinosteroid signaling2024

    • Author(s)
      Tachibana Ryo、Abe Susumu、Marugami Momo、Yamagami Ayumi、Akema Rino、Ohashi Takao、Nishida Kaisei、Nosaki Shohei、Miyakawa Takuya、Tanokura Masaru、Kim Jong-Myong、Seki Motoaki、Inaba Takehito、Matsui Minami、Ifuku Kentaro、Kushiro Tetsuo、Asami Tadao、Nakano Takeshi
    • Journal Title

      Nature Communications

      Volume: 15 Issue: 1 Pages: 370-370

    • DOI

      10.1038/s41467-023-44492-5

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] IMA peptides regulate root nodulation and nitrogen homeostasis by providing iron according to internal nitrogen status2024

    • Author(s)
      Ito Momoyo、Tajima Yuri、Ogawa-Ohnishi Mari、Nishida Hanna、Nosaki Shohei、Noda Momona、Sotta Naoyuki、Kawade Kensuke、Kamiya Takehiro、Fujiwara Toru、Matsubayashi Yoshikatsu、Suzaki Takuya
    • Journal Title

      Nature Communications

      Volume: 15 Issue: 1 Pages: 733-733

    • DOI

      10.1038/s41467-024-44865-4

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nitrate transport via NRT2.1 mediates NIN-LIKE PROTEIN-dependent suppression of root nodulation in Lotus japonicus2022

    • Author(s)
      Misawa Fumika、Ito Momoyo、Nosaki Shohei、Nishida Hanna、Watanabe Masahiro、Suzuki Takamasa、Miura Kenji、Kawaguchi Masayoshi、Suzaki Takuya
    • Journal Title

      The Plant Cell

      Volume: 34 Issue: 5 Pages: 1844-1862

    • DOI

      10.1093/plcell/koac046

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Brassinosteroid-induced gene repression requires specific and tight promoter binding of BIL1/BZR1 via DNA shape readout2022

    • Author(s)
      Nosaki Shohei、Mitsuda Nobutaka、Sakamoto Shingo、Kusubayashi Kazuki、Yamagami Ayumi、Xu Yuqun、Bui Thi Bao Chau、Terada Tohru、Miura Kenji、Nakano Takeshi、Tanokura Masaru、Miyakawa Takuya
    • Journal Title

      Nature Plants

      Volume: 8(12) Issue: 12 Pages: 1440-1452

    • DOI

      10.1038/s41477-022-01289-6

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Presentation] BZR/BES転写因子の3つのサブグループの機能比較解析2024

    • Author(s)
      古谷 朋之, 野﨑 翔平, 近藤 侑貴, 笠原 賢洋
    • Organizer
      第65回日本植物生理学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 転写ファミリー内での比較解析から根粒共生獲得の軌跡を探る2024

    • Author(s)
      野﨑 翔平, 野田 桃菜, 小野田 浩宜, 三浦 謙治, 壽崎 拓哉
    • Organizer
      日本農芸化学会2024年度大会 創立100周年記念大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] これから100年の農芸化学研究を展望する "食の未来に資する植物タンパク質研究"2024

    • Author(s)
      野﨑 翔平
    • Organizer
      日本農芸化学会2024年度大会 創立100周年記念大会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] Molecular and structural mechanism underlying the brassinosteroid-responsive transcriptional regulation by the master transcription factor BIL1/BZR12023

    • Author(s)
      Nosaki Shohei
    • Organizer
      The 24th International Conference on Plant Growth Substances
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis for moleculae mechaniss of chlorophyll biosynthesis regulation via BPGs, novel brassinosteroid signaling factors2023

    • Author(s)
      Ryo Tachibana, Susumu Abe, Momo Marugami, Ayumi Yamagami, Shohei Nosaki, Takuya Miyakawa, Takehito Inaba, Minami Matsui, Kentaro Ifuku, Ryouichi Tanaka, Tetsuo Kushiro, Tadao Asami, Takeshi Nakano
    • Organizer
      The 24th International Conference on Plant Growth Substances
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 根粒形成に必要不可欠な鍵転写因子の生化学的研究2023

    • Author(s)
      野﨑 翔平, 野田 桃菜, 三浦 謙治, 壽崎 拓哉
    • Organizer
      日本農芸化学会関東支部2023年度大会
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A nitrogen-induced peptide mediates nutrient-dependent regulation of root nodule symbiosis2023

    • Author(s)
      Momoyo Ito, Yuri Tajima, Mari Ogawa-Ohnishi, Hanna Nishida, Shohei Nosaki, Momona Noda, Naoyuki Sotta, Kensuke Kawade, Takehiro Kamiya, Toru Fujiwara, Yoshikatsu Matsubayashi, Takuya Suzaki
    • Organizer
      15th European Nitrogen Fixation Conference
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A novel motif of NIN determines its different DNA-binding specificity from NLPs2023

    • Author(s)
      Nosaki Shohei, Noda Momona, Miura Kenji, Suzaki Takuya
    • Organizer
      15th European Nitrogen Fixation Conference
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ゼニゴケにおいて配偶子器発生を制御する非典型 BZR転写因子のオーソログの分子機能比較解析2023

    • Author(s)
      古谷 朋之, 梅北 葵衣, 岩佐 碧, 野﨑 翔平, 杉本 貢一, 近藤 侑貴, 笠原 賢洋
    • Organizer
      日本植物学会 第87回大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] NIN 転写因子の特異的配列に基づく遺伝子発現制御機構2023

    • Author(s)
      野﨑 翔平, 野田 桃菜, 三浦 謙治, 壽崎 拓哉
    • Organizer
      植物微生物研究会 第32回研究交流会
    • Related Report
      2023 Annual Research Report
  • [Presentation] "Shaping" gene regulations for the second green revolution2023

    • Author(s)
      Nosaki Shohei
    • Organizer
      TSUKUBA CONFERENCE 2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 根粒形成を促進する鍵転写因子のDNA結合特性に関する研究2023

    • Author(s)
      野崎 翔平, 野田 桃菜, 三浦 謙治, 壽崎 拓哉
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Research-status Report
  • [Presentation] 硝酸態窒素による根粒形成制御に関わる自然変異の同定2023

    • Author(s)
      伊藤 百代, 番場 大, 陳 漢謀, 野崎 翔平, 田島 由理, 三浦 謙治, 佐藤 修正, 壽崎 拓哉
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Research-status Report
  • [Presentation] ブラシノステロイド新規シグナル伝達因子BPG4によるクロロフィル生合成制御を介した葉緑体恒常性維持メカニズムの解明2023

    • Author(s)
      立花 諒, 阿部 晋, 丸上 萌々, 山上 あゆみ, 野崎 翔平, 宮川 拓也, 稲葉 丈人, 松井 南, 久城 哲夫, 伊福 健太郎, 浅見 忠男, 中野 雄司
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Research-status Report
  • [Remarks] 【プレスリリース】土壌から吸収する? それとも 微生物からもらう?

    • URL

      https://www.tsukuba.ac.jp/journal/pdf/p20220212000000.pdf

    • Related Report
      2022 Research-status Report
  • [Remarks] 【プレスリリース】植物の生長を促す植物ホルモンの遺伝子発現調節機構の新しい仕組み

    • URL

      https://www.tsukuba.ac.jp/journal/pdf/p20221228140000.pdf

    • Related Report
      2022 Research-status Report

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Published: 2022-04-19   Modified: 2025-01-30  

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