| Project/Area Number |
22K14850
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 38050:Food sciences-related
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2023: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2022: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | Sphingomyelin synthase / wakame / LC-MS / fatty acid / fatty liver / obesity / NMR / chromatography / Wakame / Obesity / sphingolipid / LCMS |
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| Outline of Research at the Start |
This research aims to perform screening and identification of the active constituent in Wakame and examine its in vitro effects on lipid droplets. Next, determine the altered lipid biomarkers LC/MS and perform in vivo effects by oral feeding experiments using a High-fat diet rat model.
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| Outline of Final Research Achievements |
In this research study, we have successfully established a sphingomylein synthase(SMS) inhibition screening assay using HeLa cells by a targeted liquid-chromatography/mass spectrometry. The method establishment results were published in a peer-reviewed international journal. Then, the wakame extract was screened for SMS inhibition. The results showed the wakame hexane extract with subfraction 7 was found to be active for SMS inhibition. Further, the active compound in hexane fraction 7 was purified by column chromatography and identified as a fatty acid WH73 by NMR and HRMS characterizations. Purified compound WH73 showed good inhibition for both SMS1 and SMS2 respectively. We are now performing the in vitro results of WH73 using fatty liver cell model. Overall, the possible compound from wakame that should have a possible role in obesity control associated with lipid metabolism was reveled.
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| Academic Significance and Societal Importance of the Research Achievements |
Obesity is a leading risk factor for premature death worldwide. Dietary seaweed have a positive role in controlling the obesity, but the exact mechanisms are unknown. In this study, we have revealed these connections by identifying the active component of wakame that can act on lipid metabolism.
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