Development of novel Immunosynbiotics with wakame utilizing immunobiotics and immunogenics
| Project/Area Number |
22K14982
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 42010:Animal production science-related
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| Research Institution | Tohoku University |
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Principal Investigator |
Binghui Zhou 東北大学, 農学研究科, 助教 (70906031)
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2022: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | wakame assimilation / immunobiotic / immunosynbiotic / porcine / immune response / Immunobiotics / Immunosynbiotics / Wakame / Lactobacilli / Porcine intestinal cell / Tolerance to acid / Porcine / Probiotics / Immunogenics |
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| Outline of Research at the Start |
In order to minimize the impact of antibiotic resistance in livestock production, immunosynbiotics have been proposed to be candidates for antibiotic substitutes referring to immunomodulatory microorganisms (immunobiotics) and wakame (immunoprebiotics). I aim to determine sugar utilization and host immune modulatory patterns of selected immunobiotic strains using biochemical, genomic and immunological approach. Finally, the efficacy of the immunosynbiotics will be evaluated using in vitro and in vivo porcine models to improve the healthy growth of antibiotic-independent porcine production.
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| Outline of Annual Research Achievements |
Antibiotic resistance caused by the overuse of antibiotics in livestock production has been a severe problem, leading to the need for effective alternatives. A promising alternative to antibiotics is the "immunosynbiotics," a combination of immunobiotics and immunoprebiotics. The seaweed wakame is considered an immunoprebiotic, and L. salivarius is an immunobiotic, isolated from the intestine of wakame-fed pigs. Adding wakame to pig feed has been found to improve the immune response and alter the microbiota in the porcine large intestine. Using wakame as a feed supplement could also provide an economic recovery alternative in Miyagi prefecture after the 2011 Tohoku earthquake. These results were achieved in this study:
(1) Eight lactobacilli strains thrived better in wakame-based broths with added B vitamins, showcasing varying wakame utilization abilities based on genomic analysis. (2) A strain with adhesion to porcine intestinal epithelial cells survived simulated gastric juice with wakame due to its fiber content shielding cells from acidic conditions, facilitating potential probiotic effects. (3) We conducted an in vivo study by feeding 36 piglets with wakame fermentation using this promising strain. Furthermore, our observations demonstrated that the abundance of beneficial Lactobacillus bacteria increased, while pathogenic strains such as E. coli decreased.
The results indicate that the potential applications of immunosynbiotics in promoting overall well-being are exciting areas for further investigation and could lead to practical ways to enhance health in the future.
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Report
(2 results)
Research Products
(4 results)
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[Presentation] Isolation and immunosynbiotic characterization of lactic acid bacteria from swine milk to generate a Lactobacillus library2023
Author(s)
Binghui Zhou, Ryusuke Ohgi, Taiga Sakuma, Sudeb Saha, Mitsuki Sakurai, Yuka Nakano, Fu Namai, AKM, Humayun Kober, Wakako Ikeda-Ohtsubo, Keita Nishiyama, Haruki Kitazawa
Organizer
FEMS2023
Related Report
Int'l Joint Research
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