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Epidemic Dynamics of Swine Influenza Virus Infections in Pig Farms

Research Project

Project/Area Number 22K14995
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 42020:Veterinary medical science-related
Research InstitutionKagoshima University

Principal Investigator

Okuya Kosuke  鹿児島大学, 農水産獣医学域獣医学系, 助教 (10907736)

Project Period (FY) 2022-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2022: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords豚インフルエンザウイルス / 豚呼吸器複合病 / 豚サーコウイルス / 豚繁殖・呼吸障害症候群ウイルス / PRDC / 養豚場 / 流行動態
Outline of Research at the Start

豚インフルエンザは、豚呼吸器複合病 (PRDC) の原因となるだけでなく、人獣共通感染症でもある。2009年にはパンデミックを引き起こし、現在も養豚場の豚から新たな遺伝子再集合体が分離されている。しかしながら、養豚場内におけるSIVの感染伝播様式は未だ分かっておらず、SIV感染を制御できていない現状がある。本研究では、口腔液を検体とした簡易なSIV分離法を新たに確立し、養豚場内におけるSIVの流行動態を把握することを目的とする。さらに、豚の口腔液中に含まれるウイルスおよび細菌の遺伝子量を定量することで、PRDC発症のリスク評価として口腔液の有用性を検証する。

Outline of Final Research Achievements

We established a method for collecting oral fluids from pigs using ropes. We also evaluated the clinical scores of respiratory symptoms such as coughing and sneezing, investigating the relationship between various pathogens and clinical symptoms. In this study, swine influenza viruses (SIVs) of H1N1 and H1N2 subtypes were isolated, and two H1N2 subtype SIVs were detected. The detection of SIV genes just before the clinical score for coughing increased suggests that SIV may be a cause of early respiratory symptoms. Measuring the neutralizing antibody titers against various strains of SIV revealed that different types and levels of antibodies were present, indicating that multiple SIV strains can circulate within a single farm.

Academic Significance and Societal Importance of the Research Achievements

豚インフルエンザは、豚呼吸器複合病 (PRDC) の原因となるだけでなく、人獣共通感染症でもある。2009年にはパンデミックを引き起こし、現在も養豚場の豚から新たな遺伝子再集合体が分離されている。しかしながら、養豚場内におけるSIVの感染伝播様式は未だ分かっておらず、豚インフルエンザウイルス(SIV)感染を制御できていない現状がある。本研究では、口腔液を検体とした簡易なSIV分離法を新たに確立し、養豚場内におけるSIVの流行動態を把握した。さらに、豚の口腔液中に含まれるウイルスおよび細菌の遺伝子量を定量することで、PRDC発症のリスク評価として口腔液の有用性を検証した。

Report

(3 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • Research Products

    (10 results)

All 2023 2022 2021

All Journal Article (7 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 7 results,  Open Access: 5 results) Presentation (3 results)

  • [Journal Article] Stability of the Virucidal Activity of Commercial Disinfectants against Avian Influenza Viruses under Different Environmental Conditions2023

    • Author(s)
      Khalil Ahmed Magdy、Esaki Mana、Okuya Kosuke、Ozawa Makoto
    • Journal Title

      Pathogens

      Volume: 12 Issue: 12 Pages: 1382-1382

    • DOI

      10.3390/pathogens12121382

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Surveillance, Isolation, and Genetic Characterization of Bat Herpesviruses in Zambia2023

    • Author(s)
      Harima H, Qiu Y, Yamagishi J, Kajihara M, Changula K, Okuya K, Isono M, Yamaguchi T, Ogawa H, Nao N, Sasaki M, Simulundu E, Mweene AS, Sawa H, Ishihara K, Hang’ombe BM, Takada A
    • Journal Title

      Viruses

      Volume: 15 Issue: 6 Pages: 1369-1369

    • DOI

      10.3390/v15061369

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Newly emerged genotypes of highly pathogenic H5N8 avian influenza viruses in Kagoshima prefecture, Japan during winter 2020/212023

    • Author(s)
      Okuya Kosuke、Khalil Ahmed Magdy、Esaki Mana、Nishi Natsuko、Koyamada Donna、Saito Rara、Tokorozaki Kaori、Hasegawa Taichi、Ozawa Makoto
    • Journal Title

      Journal of General Virology

      Volume: 104 Issue: 6 Pages: 00-00

    • DOI

      10.1099/jgv.0.001870

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic characterization of bovine respiratory syncytial viruses in Japan between 2017 and 20192023

    • Author(s)
      Mitarai Sumire、Okuya Kosuke、Miyane Kazuhiro、Miyamoto Machiko、Ishikawa Shingo、Kawaguchi Hiroaki、Hatazoe Itaru、Suda Yasuo、Arima Eriko、Nakazato Hiroshi、Hobo Seiji、Masatani Tatsunori、Ozawa Makoto
    • Journal Title

      Archives of Virology

      Volume: 168 Issue: 2

    • DOI

      10.1007/s00705-022-05670-w

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] The Amino Acid at Position 95 in the Matrix Protein of Rabies Virus Is Involved in Antiviral Stress Granule Formation in Infected Cells.2022

    • Author(s)
      Kojima I, Onomoto K, Zuo W, Ozawa M, Okuya K, Naitou K, Izumi F, Okajima M, Fujiwara T, Ito N, Yoneyama M, Yamada K, Nishizono A, Sugiyama M, Fujita T, Masatani T.
    • Journal Title

      J Virol.

      Volume: 96(18) Issue: 18

    • DOI

      10.1128/jvi.00810-22

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Multiple Routes of Antibody-Dependent Enhancement of SARS-CoV-2 Infection2022

    • Author(s)
      Okuya Kosuke、Hattori Takanari、Saito Takeshi、Takadate Yoshihiro、Sasaki Michihito、Furuyama Wakako、Marzi Andrea、Ohiro Yoichi、Konno Satoshi、Hattori Takeshi、Takada Ayato
    • Journal Title

      Microbiology Spectrum

      Volume: 10 Issue: 2

    • DOI

      10.1128/spectrum.01553-21

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Serological and molecular epidemiological study on swine influenza in Zambia2021

    • Author(s)
      Harima Hayato、Okuya Kosuke、Kajihara Masahiro、Ogawa Hirohito、Simulundu Edgar、Bwalya Eugene、Qiu Yongjin、Mori‐Kajihara Akina、Munyeme Musso、Sakoda Yoshihiro、Saito Takehiko、Hang'ombe Bernard M.、Sawa Hirofumi、Mweene Aaron S.、Takada Ayato
    • Journal Title

      Transboundary and Emerging Diseases

      Volume: - Issue: 4

    • DOI

      10.1111/tbed.14373

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 呼吸器症状を呈する離乳豚に感染していたワクチン株様豚繁殖・呼吸障害症候群ウイルスの性状解析2023

    • Author(s)
      合田祈、児島一州、江嵜真南、奥谷公亮、小澤真
    • Organizer
      第166回日本獣医学会学術集会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 鹿児島県のコウモリにおけるウイルス種の多様性2023

    • Author(s)
      奥谷公亮、佐多翔、江嵜真南、児島一州、村上晋、梶原将大、宮崎和雄、船越公威、小澤真
    • Organizer
      第70回日本ウイルス学会学術集会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ザンビアにおけるブタインフルエンザの疫学調査2023

    • Author(s)
      播磨勇人、奥谷公亮、梶原将大、小川寛人、Edgar Simulundu、Eugene Bwalya、邱永晋、迫田義博、西藤岳彦、Hang’ombe Bernard、澤洋文、Aaron Mweene、高田礼人
    • Organizer
      第166回日本獣医学会学術集会
    • Related Report
      2023 Annual Research Report

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Published: 2022-04-19   Modified: 2025-01-30  

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