| Project/Area Number |
22K15058
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 43030:Functional biochemistry-related
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Project Status |
Discontinued (Fiscal Year 2023)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2022: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | Mitophagy / Atg32 / Ppg1 / The Far complex / Yeast / Autophagy |
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| Outline of Research at the Start |
Mitophagy contributes to maintaining mitochondrial quality and quantity. The phosphorylation of the Atg32 is essential for mitophagy and is antagonistically regulated by CK2 and Ppg1. The Ppg1-binding partner Far complex is involved in Atg32 dephosphorylation. Apart from Atg32-Far8, interaction important for mitophagy regulation, it was found that Far3/Far7 interact with Atg32 independently of Far8. Thus, this study aims to clarify
① the structural basis for interaction between Far3/Far7 and Atg32
② the upstream signaling pathway regulating the dissociation between the Far complex and Atg32
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| Outline of Annual Research Achievements |
Mitophagy sustains mitochondrial quality and quantity via Atg32 phosphorylation, involving the enigmatic Far complex interaction. This study seeked to clarify: 1) Far3/7's interplay with Atg32 2) The upstream signaling guiding this interaction.
Interestingly, experiments with Far3/7 phosphorylation mutants revealed no disruption in the Atg32-Far complex interaction. Exploring Far3/7 expression and degradation effects yielded no discernible impact. Likewise, autophagy-associated kinase assessment failed to influence the Atg32-Far complex connection. These findings underscore the intricate regulatory mechanisms behind mitophagy. While specifics of Atg32-Far complex interaction remain elusive, this study contributes to our understanding of mitochondrial quality and quantity control.
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