| Project/Area Number |
22K15065
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 43030:Functional biochemistry-related
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
Dindo Mirco 沖縄科学技術大学院大学, タンパク質工学・進化ユニット, 客員研究員 (10933088)
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2023: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2022: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | LLPS / enzymes / catalytic efficiency / liquid droplets / pH gradient / Phase separation / Chemotaxis / TCA cycle / Enzymes / Catalysis / phase separation / protocells / cytosol fluidification / protocells migration / chemical gradient / single droplet kinetic / protein expression |
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| Outline of Research at the Start |
First year:
-Evaluation of enzymatic performance inside LLPS droplets
-Single droplet kinetics and study of the small molecule
Second year:
-Protein expression and folding studies inside the droplets
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| Outline of Final Research Achievements |
The current research project explored the function fo the enzymes within liquid droplets. In detail, we have studied the kinetic parameters of enzymes partitioned within liquid protein crowded droplets generated through liquid-liquid phase separation.
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| Academic Significance and Societal Importance of the Research Achievements |
The current project gives a better understanding about how enzymes works in liquid droplets. The liquid droplets are a new biochemical organization of the metabolism recently reported across all the living forms.
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