| Project/Area Number |
22K15085
|
|---|
| Research Category |
Grant-in-Aid for Early-Career Scientists
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Basic Section 43050:Genome biology-related
|
|---|
| Research Institution | Okinawa Institute of Science and Technology Graduate University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2022-04-01 – 2024-03-31
|
| Project Status |
Completed (Fiscal Year 2023)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2023: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2022: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | RNA editing / cephalopods / single-cell mRNA / neural organs / Cephalopod / Neural organs / Single-cell RNA / Cephalopods / Single-cell sequencing / thermal adaptability |
|---|
| Outline of Research at the Start |
Cephalopods are poikilotherms and need to regulate different neurophysiological processes at different temperatures. One proposed adaptive mechanism is the A-to-I messenger RNA editing. This project aims to understand how RNA-editing is regulated and the cell-specificity of this mechanism in coleoid cephalopods.
The results of this project will unravel how isoforms from different cell types with different RNA-editing levels influence the gene regulatory networks, enabling cephalopods adapt physiologically to the temperature variation.
|
| Outline of Final Research Achievements |
I successfully standardized a protocol for the isolation and sorting of high-quality single cells from the optic lobe of squid. I have also identified distinct cellular populations in squids that are 1 to 7 days old, differing in size and granularity. Sequencing the mRNA of these high-quality cells revealed isoform-specific editing in dopaminergic gene markers such as Ty3h, particularly in the UTR regions. Additionally, we found that RNA editing occurs significantly in transposable elements without a clear pattern of cell-type specificity.
|
| Academic Significance and Societal Importance of the Research Achievements |
We found that dopaminergic cell types, the most abundant in the optic lobe, exhibit differential RNA-editing patterns in gene markers, particularly in regulatory regions. We expect to fully characterize cephalopod-specific and evolutionarily conserved regulatory networks that underlie RNA editing.
|
