| Project/Area Number |
22K15150
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 44030:Plant molecular biology and physiology-related
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Coleman Duncan 国立研究開発法人理化学研究所, 環境資源科学研究センター, 特別研究員 (10895311)
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| Project Period (FY) |
2022-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2024: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Regeneration / Arabidopsis / HSF / Plant / Plants / Development / Wounding / SUMO |
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| Outline of Research at the Start |
After being cut, plants can easily regenerate new organs (e.g. shoots) from the cut site. I am researching how plants sense wounding and how they can use this signal to reactivate developmental processes to make new organs. To do this, I use the model plant species Arabidopsis and combine genetic, bioinformatic and biochemical methods to study new transcriptional regulators of regeneration. I am also interested in how the modification of these regulatory proteins affect their ability to induce regeneration.
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| Outline of Annual Research Achievements |
In FY 2022 My main contribution to this project is characterizing how the wound-activated HEAT SHOCK FACTOR A1 (HSFA1) promotes shoot regeneration in Arabidopsis. I utilized a combination of genetic, biochemical and bioinformatic tools to reveal a novel mechanism of wounding-induced shoot regeneration.
Key research findings: 1) Determined key downstream targets of HSFA1 using a bioinformatics approach. 2) Verified HSFA1 binding to direct targets using biochemistry. 3) Generated and phenotyped genetic crosses to confirm epistasis of genes involved in wound-induced cellular reprograming. 4) Observed the nuclear translocation of HSFA1d in response to wounding using live -The results have been compiled into a manuscript for submission in 2023
Collaborations (informal)
1) Ari Sadanandom (Durham University, UK) – Shared plant material and jointly wrote a grant proposal to Biotechnology and Biological Sciences Research Council (BBSRC) to investigate SUMOylation in cellular reprograming. 2) Teruaki Taji (Tokyo University of Agriculture) – Provided cDNA sample for qPCR analysis to investigate the role of HSFA1 in photomorphogenesis. 3) Yutaka Kodama (Utsunomiya University) – Visited laboratory and learned techniques for imaging leave epidermal cells with a confocal microscope.
Conferences: 1)Oral - Annual conference for Society of Experimental Biology, Montpellier France.2) Oral - Satellite meeting on SUMO modification in cell signalling – Society of Experimental Biology, Montpellier France – Invited speaker. 3)Poster - CSRS Annual progress meeting 2022
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