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The effects of leaf litter phytochemistry on the structure and functioning of microbial decomposer communities.

Research Project

Project/Area Number 22K15186
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 45040:Ecology and environment-related
Research InstitutionOkinawa Institute of Science and Technology Graduate University

Principal Investigator

ARMITAGE David  沖縄科学技術大学院大学, 統合群集生態学ユニット, 准教授 (40912390)

Project Period (FY) 2022-04-01 – 2025-03-31
Project Status Granted (Fiscal Year 2023)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2023: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2022: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Keywordsphytochemistry / chemical ecology / botany / community ecology / ecology / community / Decomposition / Phytochemistry / Microbial ecology
Outline of Research at the Start

I will first investigate whether anthropogenic forcing on plant communities impacts the distribution of phytochemicals across the landscape of Okinawa. Next, I will experimentally determine whether leaf chemistry selects for optimized microbial decomposer communities.

Outline of Annual Research Achievements

We have characterised the phytochemistry of approximately 200 native plant species on Okinawa (primarily trees, shrubs, and ferns). This work remains ongoing. Initial proof-of-concept is focused on mangrove trees but will be expanded to all habitats once a reliable analytical pipeline is established.

Presentation of these results was carried out at two international meetings in the USA and Japan.

For the decomposition component of the project, we successfully ran a study to measure the decomposition rates of the plant Crotalaria juncea, either with or without the production of the toxic alkaloid monocrotaline. Preliminary results suggest that the presence of the alkaloid slows decomposition. Further confirmatory studies are underway, and the microbiome data will be sequenced soon.

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

Delays have only occurred due to the breakdown or replacement of machinery.

Strategy for Future Research Activity

We will focus on site surveys to link the chemical data we have collected to real tree communities. The participants responsible for data analysis will also be obtaining training in computational metabolomics in order to build an analytical pipeline that will facilitate compound identification, as this is currently the limiting step in progress.

Report

(2 results)
  • 2023 Research-status Report
  • 2022 Research-status Report
  • Research Products

    (5 results)

All 2024 2023 2022

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results)

  • [Journal Article] Predictive ecology and management of phyllosphere microbial communities through cross-scale synthesis2023

    • Author(s)
      Armitage David W.、Carter Morgan、Choudhury Robin A.、Remus-Emsermann Mitja、Morris Cindy E.、Leveau Johan、Kinkel Linda、Dundore-Arias J.P.
    • Journal Title

      Phytobiomes Journal

      Volume: early view Issue: 2 Pages: 145-150

    • DOI

      10.1094/pbiomes-02-23-0012-p

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Tree stress responses across biological scales: a case study in mangroves2024

    • Author(s)
      David Armitage
    • Organizer
      Ecological Society of Japan Annual Conference
    • Related Report
      2023 Research-status Report
  • [Presentation] Tree stress responses across biological scales: a case study in mangroves2023

    • Author(s)
      David Armitage
    • Organizer
      Ecological Society of America Annual Conference
    • Related Report
      2023 Research-status Report
    • Int'l Joint Research
  • [Presentation] The Struggle for Coexistence2022

    • Author(s)
      David Armitage
    • Organizer
      University of Sherbrooke, Canada
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] Confronting and Resolving the Issues of Scale in Microbial Community Ecology2022

    • Author(s)
      David Armitage
    • Organizer
      11th International Symposium on Leaf Surface Microbiology
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2022-04-19   Modified: 2024-12-25  

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