Creation and biological evaluation of nanoparticle-based artificial transcription factor
| Project/Area Number |
22K15249
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 47010:Pharmaceutical chemistry and drug development sciences-related
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2022: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | NANO-TF / Silica nanoparticles / Mitochondrial factors / Cell Imaging / Molecular modeling / medaka egg imaging / Carbon quantum dots / Silicon quantum dots / Transcription factor |
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| Outline of Research at the Start |
Cancer is the leading cause of death worldwide. Recent studies have revealed the importance of reactive oxygen species produced by mitochondrial complex I in cancer disease and their associated signaling pathways and epigenetic regulation. It is believed that this NANO-TF can control ROS levels and intracellular energy
metabolism in patients with cancer disease and provide innovative strategies for treating cancer.
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| Outline of Annual Research Achievements |
We have developed SiQDs and CQDs for bio-imaging, as well as to prepare the artificial transcription factor, NANO-TF. The QDs we used were heavy metal-free materials without the use of cadmium and mercury. Instead, we used silicon with decene molecules for surface functionalization and Pluronic F127 for the water-soluble process. Additionally, we synthesized CQDs from waste biomass materials. We tested the cytotoxicity of both materials on various cell lines and Japanese medaka fish and found that a concentration of 1mg/mL did not cause any cytotoxic effects. We used these materials to develop NANO-TF. The NANO-TF units contain drugs and mitochondrial penetrating peptides, which enable them to penetrate inside the mitochondria.
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Report
(2 results)
Research Products
(25 results)
