| Project/Area Number |
22K15563
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 50020:Tumor diagnostics and therapeutics-related
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| Research Institution | Keio University |
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Principal Investigator |
Satoru Matsuda 慶應義塾大学, 医学部(信濃町), 助教 (30594725)
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2022: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 食道癌 / リキッドバイオプシー / ctDNA / 尿中miRNA / 薬剤耐性 / 集学的治療 |
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| Outline of Research at the Start |
食道癌手術は侵襲が非常に大きいため、術前化学療法奏効例において手術を回避することが望ましい。本課題では、腫瘍由来のcell free DNA、尿に含まれる細胞外小胞(Extracellular vesicle, EV)中のmiRNA、そして癌に対する宿主反応を反映する血中凝固因子という複数のリキッドバイオプシーを組み合わせることで、NAC後の遺残腫瘍量を精度高く定量する指標の確立をめざす。
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| Outline of Final Research Achievements |
The ctDNA was examined in patients with esophageal cancer who had undergone surgery after preoperative chemotherapy at our institution. As a result, ctDNA was detected for TP53 and other mutations that are frequently present in primary tumours of oesophageal squamous cell carcinoma. Furthermore, the presence or absence of ctDNA at 1-3 months post-operatively was shown to sensitively reflect early recurrence. We believe that these results demonstrate the usefulness of ctDNA as a tumour monitoring method in oesophageal squamous cell carcinoma.
For tumour monitoring using urinary miRNAs, urine was processed using a microfluidic device, and miRNA expression was comprehensively analysed by microarray in healthy subjects/advanced/early-stage cancers respectively. As a result, 15 miRNAs whose expression increased along a tumour burden were captured. This study suggests that it might be possible to determine the presence or absence of tumours using urinary miRNA profiles.
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| Academic Significance and Societal Importance of the Research Achievements |
食道癌における腫瘍モニタリング手法の開発に有用な評価方法の開発に成功した。本手法の精度をさらに向上させていくことにより、薬物療法奏効例への手術回避戦略の実現が可能となると考えている。
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