| Project/Area Number |
22K16010
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 53010:Gastroenterology-related
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2022: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 膵癌 / 低侵襲診断 / 遺伝子変異 / デジタルPCR / 胆道癌 / ゲノム診断 / 膵胆道癌 |
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| Outline of Research at the Start |
膵胆道系腫瘍の確定診断における細胞・組織診では、検体不適正（insufficient material）と判定される場合が少なくない。その原因は、採取可能な細胞・組織量が少ないことに起因するが、微量検体における診断上の問題を克服するため、ごく少数の癌細胞のもつ遺伝子異常を高感度検出する「デジタル細胞・組織診」を提案する。
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| Outline of Final Research Achievements |
In the definitive diagnosis of pancreatic tumors, histopathological evaluations often deem samples inadequate, typically due to the limited amount of tissue that can be collected. To overcome this challenge, this study employed digital PCR, which excels in the absolute quantification of minute quantities of nucleic acids, aiming to construct a system for the rapid and reliable detection of driver gene mutations in small cell and tissue samples. By multiplexing the system for absolute quantification of genetic abnormalities, including KRAS mutations, we confirmed that it is possible to simultaneously detect KRAS and GNAS mutations and identify the mutation types in less than 1ng of nucleic acids.
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| Academic Significance and Societal Importance of the Research Achievements |
複数の遺伝子異常を同時検出できれば形態学に基づく画像・病理診断を補填し、がん診療に大きく貢献できる。試料採取現場で実施可能なオンサイト検査法とするには、turn around timeを含む課題があるため、今後は反応系の高速化を検討している。
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