Offer insight the mechanism of hepatitis B virus elimination by a novel regulated cell death type: ferroptosis.
| Project/Area Number |
22K16033
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 53010:Gastroenterology-related
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
闕 路晟 国立感染症研究所, ウイルス第二部, 研究員 (20941636)
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| Project Period (FY) |
2022-04-01 – 2025-03-31
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| Project Status |
Granted (Fiscal Year 2022)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2024: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2023: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | HBV / Ferroptosis / infected / clearance / ferroptosis |
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| Outline of Research at the Start |
Hepatitis B virus is a causative agent of serious liver illness. Current antiviral drugs have failed to eradicate infected cells. Focusing on a novel type of cell death, the mechanism how HBV and host immune response sensitize the infected cells to ferroptosis will be investigated.
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| Outline of Annual Research Achievements |
The Hepatitis B virus is a causative agent of Chronic hepatitis B, liver cirrhosis, and hepatocellular carcinoma. Current antiviral drugs interfering viral life cycle, have failed to eradicate infected cells, and the strategies to selectively eliminate infected cells have been awaited. Focusing on a novel type of cell death, the mechanism of how HBV and host immune response sensitize the infected cells to ferroptosis will be investigated.
This year, we found a couple of anti/pro-ferroptotic genes expression is differentially expressed between no-HBV&HBV&HBV+anti-viral drug-treated in a dry/wet analysis; When a human hepatocyte cell line HepG2-NTCP was infected with HBV, a ferroptosis agonist and antagonist decreased and increased HBV infection by in-vitro experiment, respectively;
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
We can spend more time in the laboratory to carry forward this project this year compared with one year ago during the SARS-CoV-2 pandemic.
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| Strategy for Future Research Activity |
We have currently identified a couple of anti/pro-ferroptotic genes which involved in HBV infection. In the next year, we try to clarify the role of those genes in HBV infection by performing a lost/gain of function approach. The effect of these genes on viral markers and cell viability will be quantified.
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Report
(1 results)
Research Products
(4 results)
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[Journal Article] Interferon-gamma induced APOBEC3B contributes to Merkel cell polyomavirus genome mutagenesis in Merkel cell carcinoma2021
Author(s)
Que L, Li Y, Dainichi T, Kukimoto I, Nishiyama T, Nakano Y, Shima K, Suzuki T, Sato Y, Horike S, Aizaki H, Watashi K, Kato T, Aly HH, Watanabe N, Kabashima K, Wakae K, Muramatsu M.
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Journal Title
J Invest Dermatol
Volume: S0022-202X(21)
Issue: 7
Pages: 02636-1
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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