| Project/Area Number |
22K16341
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 54020:Connective tissue disease and allergy-related
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
Lee Wen Shi 東京医科歯科大学, 大学院医歯学総合研究科, 非常勤講師 (30883590)
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2022: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Rheumatoid Arthritis / Lactosome / Drug Delivery System / miRNA-9 / RasGRP4 / rheumatoid arthritis / arthritis mouse model / drug delivery system / lactosome |
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| Outline of Research at the Start |
1. The delivery of miR-9 using lactosome (Kyoto University-Shimadzu Product) in RASFs
2. The effects of lactosome-miR-9 on RASFs
3. Lactosome-miR-9 in CIA mice
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| Outline of Final Research Achievements |
Lactosome-miR-9 was injected intravenously into a collagen-induced arthritis mouse model, with lactosome-control siRNA serving as the control group. The mean arthritic score was relatively lower in the miR-9 group compared to the control group. Histological assessments of the joints in the lactosome-miR-9 group showed reduced inflammation. STIA mice was used to induce arthritis and repeated the experiments to confirm the efficacy of lactosome-miR-9 in a different arthritis model. We investigated the potential of targeting RasGRP4 using lactosome:
RasGRP4 deficiency attenuated both K/BxN STIA and AIA without suppressing adaptive immune responses.
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| Academic Significance and Societal Importance of the Research Achievements |
我々の研究成果の科学的および社会的意義は、関節炎治療における新たなアプローチを提供する点にあります。ラクトソームを用いたmiR-9の投与は、炎症を効果的に抑制し、既存の治療法に対する耐性を克服する可能性を示した。さらに、RasGRP4の標的化は、免疫応答を維持しつつ関節炎の症状を軽減する新たな治療戦略となり得る。これにより、関節炎患者の生活の質向上が期待され、社会全体の健康増進に寄与する。
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