| Project/Area Number |
22K16374
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 54030:Infectious disease medicine-related
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| Research Institution | Kumamoto University |
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Principal Investigator |
Barabona Godfrey 熊本大学, ヒトレトロウイルス学共同研究センター, 特別研究員 (40906674)
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2023: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2022: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | MiRNA / HIV related inflammation / miRNA / extracellular vessicle / Inflammation / HIV / Extracellular vesicle / MicroRNA |
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| Outline of Research at the Start |
This study will analyze circulating microRNAs that are delivered by extracellular vesicles and determine their role in systemic immune dysfunction and chronic inflammation seen in HIV infection. The central hypothesis is that, in HIV infection, microRNAs are dysregulated and play a crucial role in the persistent immune dysfunction and chronic inflammation.
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| Outline of Final Research Achievements |
We have identified distinct patterns of immunoregulatory miRNA in extracellular vesicles from HIV-infected individuals which may aid to advance our knowledge on HIV pathogenesis and the related chronic inflammation. We successfully developed an invitro model that demonstrated the effects of EV derived miRNAs in cytokine secretion from THP-1 derived microphages. Additionally, we showed that some of the immunoregulatory microRNA levels in EVs were significantly associated with plasma inflammatory markers. These miRNAs could serve as non-invasive biomarkers for HIV status and progression, opening potential for targeting specific miRNAs to control inflammation in HIV patients. The next steps include studying other cell lines to confirm miRNA impact across different environments, validating miRNA roles in cytokine secretion with functional studies and clinical samples, investigating how miRNAs modulate cytokine expression for deeper insights and therapy.
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| Academic Significance and Societal Importance of the Research Achievements |
Identifying distinct miRNA patterns in EVs from HIV-infected individuals enhances our understanding of mechanisms for HIV pathogenesis. These findings can enhance treatment strategies and improve the quality of life for HIV-infected individuals by managing HIV-related inflammation.
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