| Project/Area Number |
22K16375
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 54030:Infectious disease medicine-related
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2025-03-31
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| Project Status |
Granted (Fiscal Year 2023)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2024: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2023: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2022: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | HIV-1 restriction |
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| Outline of Research at the Start |
Proposed plan is outlined to be conducted within tow years. First plan is conducted in one year, and Plane B is conducted in the second year. Plane C will be conducted alongside other plans.
By third year, it is excepected to conclude all results and prepare paper for publication.
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| Outline of Annual Research Achievements |
Results showed iPS-ML derived macrophages are useful as a good model for investigating host/HIV-1 interaction in myeloid cells. Analysis of APOBEC3 family protein in iPS-ML cell showed differential expression of individual proteins (for example: A3A, A3G, A3F), and more importantly some cell lines express stable haplotypes of A3H. Furthermore, A3 proteins (specially A3G A3F) induced HIV-1 restriction in iPS-ML derived macrophages. This restriction is counteracted by the HIV-1 viral factor Vif.
As A3 poteins mediate HIV-1 restriction via inducing lethal mutations to HIV-1 genome, G- to -A mutations are signifcantly detected in the Vif-deficient HIV-1-infected iPS-ML cells. However, deaminase-independent mechanisms (no viral mutations) to restrict HIV-1 in iPS-ML cells are also suggested.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Currently, one more staff joined this research project, which provided better handling of the planned investigations.
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| Strategy for Future Research Activity |
Future work will focus on determining the possible mechanisms to induce HIV-1 restriction in iPS-MLderived macrophages.
- analysis for A3-mediated viral mutations to identify frequency, magnitude and preferred sequences of A3 proteins to induce such restriction.
- analysis for HIV-1 reverse transcription (RT) and late RT products in order to clarify deaminase independent restriction.
- Creating modified iPS-ML derived macrophages (A3G deleted, A3F deleted, or A3A-A3G deleted cells), which will characterize the potential role of each individual protein to mediate HIV-1 restriction, as well as testing the potential to utilze A3-mediated mutagenesis to attain HIV-1 functional cure.
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