| Project/Area Number |
22K17010
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 57020:Oral pathobiological science-related
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| Research Institution | Hokkaido University |
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Principal Investigator |
ハビバ ウンマ 北海道大学, 医学研究院, 客員研究員 (40755914)
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| Project Period (FY) |
2022-04-01 – 2025-03-31
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| Project Status |
Granted (Fiscal Year 2023)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2024: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | cancer stem cells (CSCs) / DN gels / drug-resistant / keyworad / cancer stem cells / head and neck cancer / hydrogel / reprogramming |
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| Outline of Research at the Start |
Cancer recurrence can arise due to rare circulating cancer stem cells (CSCs) resistant to chemotherapies and radiotherapies. This research focuses on the induction of head and neck cancer stem cells (HNCSCs) using hydrogel as a biomaterial. This study will develop suitable methods for detecting HNCSCs to create a personalized therapeutic modality. The study aims are:
1. Investigation of the mechanism of HNCSCs induction by the hydrogel.
2. Development of a diagnostic method for HNCSCs.
3. Establishment of a drug screening system for clinical application.
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| Outline of Annual Research Achievements |
Cancer recurrence can arise due to circulating cancer stem cells (CSCs) resistant to treatment. This research focuses on inducing head and neck cancer stem cells (HNCSCs) using hydrogel as a biomaterial. The study aims to investigate the mechanism of HNCSCs induction by the hydrogel, develop a diagnostic method for HNCSCs, and establish a drug screening system for clinical application.
The proposed study will elucidate the mechanism of hydrogel-induced reprogramming of cancer cell populations to identify new CSC markers.
We characterized the hydrogel-induced sphere as forming head and neck SCC cells. We cultured 4 HNSCC cells on DN gels for 5 days and checked SOX-2, Nanog, OCt3/4, and ALDH1 expression for up to 5 days by PCR. CSC markers show elevated expression as time elapses.
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| Current Status of Research Progress |
Current Status of Research Progress
4: Progress in research has been delayed.
Reason
Currently, I cannot do research as a full-time researcher. Due to my job responsibilities, current research progress is delayed. However, I could speed up my research from August 2024 onward.
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| Strategy for Future Research Activity |
2024-2025 Fiscal Year Plan:
(I) To elucidate the mechanism of induction of CSCs by hydrogel by analysis of mechanosignal molecules and by genome/epigenome analysis
(II) To develop a CSC diagnostic method using hydrogel by Identifying new CSC markers (Microarray and RNAseq analysis will be performed) and creating a personalized medical hydrogel kit using Patient-derived primary cultured cells to determine hydrogel conditions that can predict human tumors' recurrence profile after treatment.
(III) To create seeds for CSC therapy using hydrogel by developing a drug screening system and identifying seeds using a hydrogel plate, we will do the drug screening, intending to obtain drug seeds that can be applied to medical treatment by performing large-scale screening targeting CSCs.
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