| Project/Area Number |
22K17259
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 57070:Developmental dentistry-related
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Project Status |
Discontinued (Fiscal Year 2022)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2024: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | Amleogenin / Clone / Malassez / malassez / amelogenin |
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| Outline of Research at the Start |
The importance of these three trials is that they establish a therapeutic strategy for the regeneration of the entire PDL (tissue around tooth) complex to restore the functions of the tooth. Combining these three phases could help develop a therapeutic strategy for the regeneration of hard tissue or PDL tissue, from basic research to clinical application.
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| Outline of Annual Research Achievements |
Epithelial cell rests of Malassez (ERM) are an easy source of stem cell-like cells. Our previous work has successfully demonstrated that an ERM variant (isolated clone of ERM) can maintain healthy periodontal space by inhibiting ankylosis between the tooth and alveolar bone.
This study aims to use the applicant's previously isolated clone model to repair the lost or injured periodontal ligament (PDL) tissue to restore tooth form and function.
Phase-1: We obtained primary ERM from PDL tissues of porcine and isolated clones of ERM. We isolated clones 1-10. The characterization was done using immunofluorescence staining of ERM cell markers.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
We have already isolated clones and characterized their origin as Epithelial cell rests of malassez markers.
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| Strategy for Future Research Activity |
Phase-2: ERM clones will be checked for enamel matrix proteins and some stem cell markers.
Pilot experiments to combine some cells to produce cell sheets.
Phase-3: Histological study of the transplanted tooth. Next-generation sequencing of ERM clones with stem cell-like properties and analysis to identify essential genes.
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