Establishing a low-cost next-generation sequencing platform for on-site real-time surveillance of SARS-CoV-2 and other viruses in an unreached community from the Democratic Republic of Congo
| Project/Area Number |
22K17352
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 58020:Hygiene and public health-related: including laboratory approach
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| Research Institution | Osaka Metropolitan University |
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Principal Investigator |
チバング・カバンバ エバリステ 大阪公立大学, 大学院医学研究科, 特任助教 (50900693)
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | Viral genome / Nanopore sequecing / Congo / Hepatitis B virus / Monkeypox virus / SARS-CoV-2 / Virus / Sequencing / Genome / Mbujimayi / Laboratory |
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| Outline of Research at the Start |
The outline of research is to provide a diagnostic and surveillance tool that enable generating actionable information on which to implement targeted public health strategy that will facilitate greater and early anticipation as well as initiation of effective strategies to mitigate and contain outbreaks of SARS-CoV-2 variants and other viruses.
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| Outline of Annual Research Achievements |
In FY 2023, studies were conducted in Africa applying our developed toolkit. With the decline of the SARS-CoV-2 pandemic, our interest shifted towards investigating additional endemic viruses, including hepatitis B virus, Monkeypox virus, and other viruses potentially transmitted from mother to child. Overall, 1077 individuals, comprising 200 pregnant women and 200 newborns, participated in surveys conducted in rural populations of the DRC. However, challenges arose in deploying our virus sequencing toolkit directly in the field, primarily due to the complexities of safely delivering sequencing kits in specific storage conditions. Therefore, we decided to collect and transport samples to Japan for analysis using the toolkit, albeit resulting in prolonged wait times for sample transfer.
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Report
(2 results)
Research Products
(17 results)
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[Journal Article] Comparative genomics of two Vietnamese Helicobacter pylori strains, CHC155 from a non-cardia gastric cancer patient and VN1291 from a duodenal ulcer patient.2023
Author(s)
Phuc BH, Tuan VP, Binh TT, Tung PH, Tri TD, Dung HDQ, Thuan NPM, Fauzia KA, Tshibangu-Kabamba E, Alfaray RI, Saruuljavkhlan B, Matsumoto T, Akada J, Yamaoka Y.
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Journal Title
Sci Rep
Volume: 13
Issue: 1
Pages: 8869-8869
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Differential cardiomyocyte transcriptomic remodeling during in vitro Trypanosoma cruzi infection using laboratory strains provides implications on pathogenic host responses2023
Author(s)
Candray-Medina KS, Nakagama Y, Ito M, Nakagama S, Tshibangu-Kabamba E, Takeda N, Sugiura Y, Nitahara Y, Michimuko-Nagahara Y, Kaku N, Onizuka Y, Arias CE, Mejia M, Alas K, Pena S, Maejima Y, Komuro I, Nakajima-Shimada J, Kido Y.
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Journal Title
Tropical Medicine and Health
Volume: 51
Issue: 1
Pages: 86-86
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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