| Project/Area Number |
22K18200
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 90120:Biomaterials-related
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Han Aifang 国立研究開発法人理化学研究所, 生命機能科学研究センター, 研究員 (30938832)
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| Project Period (FY) |
2022-04-01 – 2025-03-31
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| Project Status |
Granted (Fiscal Year 2023)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2023: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2022: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | micro-casting / cell morphogenesis / extracellular matrix / biomaterial / surface mobility |
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| Outline of Research at the Start |
The applicant has been designed a unique substrate. The substrate is different in surface molecular mobility relying on the stripe-patterned distribution. When cells sensitively recognized the mobility stripe via the integrin-extracellular matrix (ECM) attachment, the cell morphology may be affected, such as elongation. Through the observation, the applicant wants to point out the importance of the surface mobility in terms of cell morphogenesis.
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| Outline of Annual Research Achievements |
Micro-casting fabrication of multi collagen strips patterned surface has been
achieved. The addition of DMS-co-EO into Polydimethylsiloxane(PDMS) mold has improved the harvesting of collagen strips greatly. The underlying reason is due to the increased wettability of DMS-co-EO added PDMS mold compared to PDMS mold. The fibroblast cell elongation morphology has been observed onto the micro-patterned collagen strip surface.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
I take maternity leave and childcare leave during 2023.07-2024.03
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| Strategy for Future Research Activity |
I will continue to do other cell types (stem cell, muscle cell) to observe the cell morphology on the micropatterned extracellular matrix surface. Also, I will try different types of extracellular matrix (alumina, fibronectin) micropatterned on the device to detect the cell morphology.
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