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Active design of the Kai proteins on the basis of cross-scale causality

Research Project

Project/Area Number 22K19279
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
Research InstitutionInstitute for Molecular Science

Principal Investigator

Akiyama Shuji  分子科学研究所, 協奏分子システム研究センター, 教授 (50391842)

Project Period (FY) 2022-06-30 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2023: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2022: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywordsシアノバクテリア / 概日時計
Outline of Research at the Start

地球外環境下で生態系を構築するためには、克服すべき多くの課題が残されている。その一つは環境サイクルである。月面上の1日の長さは、地球の約1ヶ月に相当するため、概日(約24時間)周期の体内時計(概日時計)を保持する地球上の生命は適応が困難である。本課題では、シアノバクテリアの概日時計を改変して月面環境サイクル耐性を付与し、閉鎖生態系の構築に向けた足掛かりを作る。

Outline of Final Research Achievements

The cyanobacterial circadian clock system can be reconstituted in vitro by mixing three kinds of clock proteins (KaiA, KaiB, and KaiC) in the presence of ATP. Based on the cross-scale causality that connects the molecular scale to the cellular scale, the clock period was lengthened from circadian to "one-third of a month" by designing a KaiC mutant with an extensively reduced ATPase activity.

Academic Significance and Societal Importance of the Research Achievements

タンパク質分子のみから成る分子システムによって長周期リズムを実証することができれば、未だそのメカニズムが解明されていない概月リズムや概年リズムの理解深化はもとより、それらの設計原理にも指針を与え得る。

Report

(3 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • Research Products

    (10 results)

All 2024 2023 Other

All Journal Article (3 results) (of which Open Access: 3 results,  Peer Reviewed: 2 results) Presentation (6 results) (of which Invited: 4 results) Remarks (1 results)

  • [Journal Article] The priming phosphorylation of KaiC is activated by the release of its autokinase autoinhibition2024

    • Author(s)
      Furuike Yoshihiko、Onoue Yasuhiro、Saito Shinji、Mori Toshifumi、Akiyama Shuji
    • Journal Title

      biorxiv

      Volume: -

    • DOI

      10.1101/2024.03.21.584037

    • Related Report
      2023 Annual Research Report
    • Open Access
  • [Journal Article] Structure-function relationship of KaiC around dawn2024

    • Author(s)
      Furuike Yoshihiko、Yamashita Eiki、Akiyama Shuji
    • Journal Title

      Biophysics and Physicobiology

      Volume: 21 Issue: 1 Pages: n/a

    • DOI

      10.2142/biophysico.bppb-v21.0001

    • ISSN
      2189-4779
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Master allostery in clock protein KaiC orchestrates circadian rhythm2023

    • Author(s)
      Yoshihiko Furuike, Atsushi Mukaiyama, Shuji Akiyama
    • Journal Title

      SPring-8/SACLA Research Frontiers 2022

      Volume: - Pages: 26-27

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] リン酸化率依存的なKaiC-KaiA 相互作用の変調2024

    • Author(s)
      中尾有紗, 青山理紗子, 山崎洋一, 秋山修志, 米澤健人, 上久保裕生
    • Organizer
      PF研究会「物質・生命研究における小角散乱法の展開」
    • Related Report
      2023 Annual Research Report
  • [Presentation] 概日時計のこれまでとこれから2024

    • Author(s)
      秋山修志
    • Organizer
      生物科学異分野融合シンポジウム2023
    • Related Report
      2023 Annual Research Report
  • [Presentation] Autonomous Disassembly of Circadian Clock System at Dawn2023

    • Author(s)
      秋山修志
    • Organizer
      学術変革領域研究B「時間タンパク質学」領域会議2023
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] 概日時計のこれまでとこれから2023

    • Author(s)
      秋山修志
    • Organizer
      2023年生物リズム若手研究者の集い
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] ATPase Regulation in KaiC Triggers Assembly and Disassembly of Clock Proteins in Cyanobacteria2023

    • Author(s)
      Yoshihiko Furuike, Shuji Akiyama
    • Organizer
      日本生物物理学会シンポジウム
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] 概日時計のこれまでとこれから2023

    • Author(s)
      秋山修志
    • Organizer
      大学共同利用機関2023 現代の社会問題に挑む
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Remarks] 生命のリズムを化学する

    • URL

      https://groups.ims.ac.jp/organization/akiyama_g/

    • Related Report
      2023 Annual Research Report

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Published: 2022-07-05   Modified: 2025-01-30  

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