• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Role of "motivation" neurons in regulating autonomic nervous system function

Research Project

Project/Area Number 22K19709
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 59:Sports sciences, physical education, health sciences, and related fields
Research InstitutionTottori University

Principal Investigator

Koba Satoshi  鳥取大学, 農学部, 教授 (40565743)

Co-Investigator(Kenkyū-buntansha) 吉村 祐貴  鳥取大学, 医学部, 助教 (50771242)
Project Period (FY) 2022-06-30 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2023: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2022: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
KeywordsFosTRAP / 視床下部背側野 / 交感神経抑制 / 運動意欲 / 遺伝子導入 / 意欲 / 運動 / 交感神経 / 遺伝子ノックインラット / 光遺伝学 / ラット
Outline of Research at the Start

精神病態下で認められる自律神経機能不全は意欲低下の原因かつ結果である.しかし意欲と自律神経機能との連関メカニズムは不明である.本研究では,「独自確立したラットFosTRAP法」「特定神経を標識可視化・活動操作・除去する,遺伝子工学の先端技術」「in vivo生体信号記録による自律神経機能評価」を融合させた研究戦略から,「神経Xが意欲と自律神経系との因果性を担う脳実体である」「意欲にあふれた動物個体では恒常的に,神経Xが交感神経活動を適正に抑制制御している」との仮説を検証する.本研究の挑戦は,意欲低下と関連した精神病態の新しい理解につながり,また未来医療の開発基盤となる可能性を秘める.

Outline of Final Research Achievements

We used newly generated FosTRAP rats via CRISPR/Cas9 system to investigate the distribution of excited hypothalamic neurons due to the motivation for running exercise as well as their roles in eliciting sympathetic outflow. Dorsal hypothalamus (DH) of rats that performed 2-h voluntary treadmill running with tamoxifen displayed a greater number of “FosTRAPed” neurons than that of control rats in which tamoxifen was administered during the resting period on the treadmill. Under urethane anesthesia, optogenetic stimulation of cell bodies of the running-exercise-FosTRAPed DH neurons decreased renal sympathetic nerve activity. Optogenetic stimulation of axons extending from the running-exercise-FosTRAPed DH neurons in the ventral medulla produced a pressor effect. These observations suggest both sympathoinhibitory and sympathoexcitatory DH neurons play roles in determining appropriate levels of sympathetic nerve activity during motivation-driven running exercise.

Academic Significance and Societal Importance of the Research Achievements

抑うつ状態などの精神病態下で認められる自律神経機能不全は意欲低下の原因かつ結果である.しかし意欲と自律神経機能との連関メカニズムは研究自体がなかった.本研究では私たちが独自作出したラットFosTRAP法や光遺伝学などの遺伝子工学的技術を活用して,運動意欲の亢進に伴って興奮する視床下部神経による交感神経制御能を調査し,それらの役割を初めて明らかにした.本研究の知見は意欲と自律神経機能との因果性を担う脳実体に迫ったものであり,現代社会の病とも言える意欲の低下と関連した精神病態の新しい理解にもつながり,また未来医療の方策開発の基盤となる可能性も秘める.

Report

(3 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • Research Products

    (13 results)

All 2024 2023 2022

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (9 results) (of which Invited: 4 results)

  • [Journal Article] Orexinergic neurons contribute to autonomic cardiovascular regulation for locomotor exercise2024

    • Author(s)
      Narai Emi、Yoshimura Yuki、Honaga Takaho、Mizoguchi Hiroyuki、Yamanaka Akihiro、Hiyama Takeshi Y.、Watanabe Tatsuo、Koba Satoshi
    • Journal Title

      The Journal of Physiology

      Volume: -

    • DOI

      10.1113/jp285791

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Generation of <i>c-Fos</i> knockout rats, and observation of their phenotype2023

    • Author(s)
      Yoshimura Yuki、Nakamura Kazuomi、Seno Misako、Mochizuki Misa、Kawai Kenji、Koba Satoshi、Watanabe Tatsuo
    • Journal Title

      Experimental Animals

      Volume: 72 Issue: 1 Pages: 95-102

    • DOI

      10.1538/expanim.22-0077

    • ISSN
      0007-5124, 1341-1357, 1881-7122
    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A brainstem monosynaptic excitatory pathway that drives locomotor activities and sympathetic cardiovascular responses2022

    • Author(s)
      Koba Satoshi、Kumada Nao、Narai Emi、Kataoka Naoya、Nakamura Kazuhiro、Watanabe Tatsuo
    • Journal Title

      Nature Communications

      Volume: 13 Issue: 1 Pages: 5079-5079

    • DOI

      10.1038/s41467-022-32823-x

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mechanisms of autonomic cardiovascular adjustments during exercise and stress2022

    • Author(s)
      木場 智史、奈良井 絵美
    • Journal Title

      The Autonomic Nervous System

      Volume: 59 Issue: 4 Pages: 354-357

    • DOI

      10.32272/ans.59.4_354

    • ISSN
      0288-9250, 2434-7035
    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 運動興奮性の外側視床下部神経の交感神経抑制能と交感神経活性能2024

    • Author(s)
      木場智史、柴山夏蓮
    • Organizer
      第 76 回日本自律神経学会総会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 学会賞受賞講演2024

    • Author(s)
      木場智史
    • Organizer
      第 76 回日本自律神経学会総会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] Locomotion and sympathetic cardiovascular responses by orexinergic neurons in rats.2023

    • Author(s)
      Emi Narai, Yuki Yoshimura, Akihiro Yamanaka, Naoya Kataoka, Kazuhiro Nakamura, Tatsuo Watanabe, Satoshi Koba
    • Organizer
      第100回日本生理学会大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Sympathoinhibitory and sympathoexcitatory roles of running exercise-excited lateral hypothalamic neurons assessed by rat FosTRAP2023

    • Author(s)
      Satoshi Koba, Yuki Yoshimura, Kazuomi Nakamura, Misako Senoo, Karen Shibayama, Emi Narai, Takeshi Hiyama, Tatsuo Watanabe
    • Organizer
      第100回日本生理学会大会
    • Related Report
      2022 Research-status Report
  • [Presentation] セントラルコマンドを伝達する視床下部-中脳-延髄経路2023

    • Author(s)
      奈良井絵美,熊田奈桜, 吉村祐貴, 檜山武史, 渡邊達生, 木場智史
    • Organizer
      第17回環境生理学プレコングレス
    • Related Report
      2022 Research-status Report
  • [Presentation] セントラルコマンドの脳回路メカニズム:オレキシン神経系の役割2022

    • Author(s)
      奈良井 絵美, 木場 智史
    • Organizer
      第49回自律神経生理研究会
    • Related Report
      2022 Research-status Report
  • [Presentation] 心疾患と運動時交感神経制御の中枢メカニズム2022

    • Author(s)
      木場智史
    • Organizer
      第95回日本成果学会大会
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] 運動時交感神経活性の中枢回路メカニズム2022

    • Author(s)
      木場智史
    • Organizer
      第75回日本自律神経学会総会
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] 心不全や運動時の交感神経活性を司る中枢回路2022

    • Author(s)
      木場智史
    • Organizer
      第19回九州脳・高血圧・循環制御研究会
    • Related Report
      2022 Research-status Report
    • Invited

URL: 

Published: 2022-07-05   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi