Production of 2-D nanocarbons with different bioaffinities as ROS quenching agents to improve mammalian oocyte cryopreservation outcomes
| Project/Area Number |
22K20535
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0501:Physical chemistry, functional solid state chemistry, organic chemistry, polymers, organic materials, biomolecular chemistry, and related fields
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| Research Institution | Okayama University |
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Principal Investigator |
FERREPUJOL PILAR 岡山大学, 異分野融合先端研究コア, 特任助教 (50839008)
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| Project Period (FY) |
2022-08-31 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | graphene oxide / reduced grapehene oxide / cryoprotectant / oocyte / vitrification / reduced graphene oxide / in vitro maturation / cryopreservation / nanocarbons / reactive oxygen species |
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| Outline of Research at the Start |
Nanocarbons such as graphene and graphene oxide (GO) have multiple applications in the biological field. Because of their controllable surface chemistry, such as oxidation levels, and easy functionalization with other molecules, they can be used as ROS donors/quenchers.
This is of special interest in the field of germplasm cryopreservation, where the success is limited at the thawing stage, because excessive ROS production damages the cells. For this, I will study the application of graphene and GO during the thawing and recovery stages after cryopreservation to obtain high quality oocytes.
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| Outline of Annual Research Achievements |
The results indicated that when graphene oxide (GO) and reduced graphene oxide (rGO) were used during vitrification, they negatively affected the survival of cumulus-oocyte-complexes due to the strong affinity of the materials with the cell membranes. Furthermore, when the materials were added after thawing, their protective effect against reactive oxygen species could also not be clearly evaluated. The high reactivity of the surface of GO with the inorganic components of the culture media (especially with positive ions such as Na, K or Ca) could partly explain the reason why pristine GO and rGO were unable to successfully protect the oocytes from oxidative stress.
Further studies employing surface-modified GO are necessary to successfully use this material for cryopreservation.
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Report
(2 results)
Research Products
(1 results)
