• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of establishment and breakdown of phospholipid asymmetry in the plasma membrane

Research Project

Project/Area Number 22K20634
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeMulti-year Fund
Review Section 0701:Biology at molecular to cellular levels, and related fields
Research InstitutionKeio University (2023)
Kyushu University (2022)

Principal Investigator

Ono Yumiko  慶應義塾大学, 薬学部(芝共立), 特任助教 (40968442)

Project Period (FY) 2022-08-31 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords細胞内輸送 / 選別輸送 / lipid raft / スフィンゴ脂質 / 細胞極性 / 形質膜 / 脂質 / 輸送 / 生体膜 / 非対称 / 上皮細胞 / 極性
Outline of Research at the Start

細胞を包む一枚の脂質二重層は、内層と外層で異なる脂質組成を有する。さらに上皮細胞では、頂端側の形質膜にスフィンゴミエリンが豊富に存在し、微絨毛の形成に関与している。しかしながら、これらの脂質の非対称性分布の形成機構と極性を持った脂質の分布機構の関連は明らかになっていない。本研究では、形質膜の非対称性分布がどのように確立されているのかを明らかにする。

Outline of Final Research Achievements

The lipid bilayer is composed of different lipids in the inner and outer layers. In epithelial cells, it is known that different proteins and lipids are asymmetrically distributed in the apical and basolateral plasma membrane bordering the tight junction structure, called the epithelial cell polarity.
In this research project, we have been investigating the molecular mechanisms underlying the asymmetric distribution of plasma membrane components.

Academic Significance and Societal Importance of the Research Achievements

本研究は長らく信じられてきたlipid raftの概念を詳細に解析する。上皮細胞極性の形成・維持の根底にある分子メカニズムは、発生やがんに関連する。これらの経路を操作する治療戦略の開発には、分子基盤の解明が不可欠であり、本研究はその基礎となる知見をもたらす。

Report

(3 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • Research Products

    (3 results)

All 2023 2022

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (2 results)

  • [Journal Article] Cholesterol-rich domain formation mediated by ZO proteins is essential for tight junction formation2023

    • Author(s)
      Shigetomi Kenta、Ono Yumiko、Matsuzawa Kenji、Ikenouchi Junichi
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 120 Issue: 8

    • DOI

      10.1073/pnas.2217561120

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] mTORC2 suppresses cell death induced by hypo-osmotic stress by promoting sphingomyelin transport2022

    • Author(s)
      小野由美子, 松沢健司, 池ノ内順一
    • Organizer
      第74回日本細胞生物学会大会
    • Related Report
      2022 Research-status Report
  • [Presentation] mTORC2 suppresses cell death induced by hypo-osmotic stress by promoting sphingomyelin transport2022

    • Author(s)
      小野由美子, 松沢健司, 池ノ内順一
    • Organizer
      第45回日本分子生物学会年会
    • Related Report
      2022 Research-status Report

URL: 

Published: 2022-09-01   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi