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Effects of cytokine-stimulated MSC-derived extracellular vesicles on bone-tendon healing of shoulder rotator cuff tears.

Research Project

Project/Area Number 22K20937
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeMulti-year Fund
Review Section 0905:Surgery of the organs maintaining homeostasis and related fields
Research InstitutionOsaka University

Principal Investigator

Hanai Hiroto  大阪大学, 医学部附属病院, 医員 (90964437)

Project Period (FY) 2022-08-31 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2023: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2022: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Keywordsエクソソーム / 間葉系間質細胞 / 肩腱板断裂 / 間葉系幹細胞
Outline of Research at the Start

間葉系間質細胞(mesenchymal stroma cell, MSC)は,エクソソームを主体とする細胞外小胞(small extracellular vesicles, sEVs)を分泌し,細胞間の情報伝達を行うことで,組織修復に寄与することが知られており,運動器組織に対する再生医療としても注目を集めている.一方で,肩腱板断裂は自然治癒せず,修復手術でも再断裂をきたすことがあり有効な治療法はない.本研究の目的は,サイトカインでプレコンディショニングされたMSCから得られたsEVsが骨-腱修復に与える影響がいかに変化するかを明らかにすることである.

Outline of Final Research Achievements

Mesenchymal stromal cells (MSCs) secrete small extracellular vesicles (sEVs), mainly exosomes, and are known to contribute to tissue repair by transmitting information between cells. MSCs have attracted attention as a regenerative medicine for musculoskeletal tissues such as rotator cuff tears. In this field, different sEVs secreted by MSCs under different culture conditions have not been evaluated. In this study, it was revealed that sEVs secreted by MSCs preconditioned with IFN-γ had altered secretion amount and protein composition compared to normal sEVs.

Academic Significance and Societal Importance of the Research Achievements

近年,肩腱板断裂に対して,関節鏡を用いた修復術の発展により低侵襲治療が可能となってきたが,実際には20%程度の頻度で再断裂を合併し,術後の肩安静固定期間が数ヶ月に及んでいる現状がある.本研究で得られた研究成果は,今後臨床現場においても質の良い腱板の短期的な修復の新たな知見になると考えられる.

Report

(3 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • Research Products

    (5 results)

All 2023 2022

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Small extracellular vesicles derived from human adipose‐derived mesenchymal stromal cells cultured in a new chemically‐defined contaminate‐free media exhibit enhanced biological and therapeutic effects on human chondrocytes in vitro and in a mouse osteoarthritis model2023

    • Author(s)
      Hanai Hiroto、Hart David A.、Jacob George、Shimomura Kazunori、Ando Wataru、Yoshioka Yusuke、Ochiya Takahiro、Nakagawa Shinicihi、Nakamura Masato、Okada Seiji、Nakamura Norimasa
    • Journal Title

      Journal of Extracellular Vesicles

      Volume: 12 Issue: 7

    • DOI

      10.1002/jev2.12337

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Clinical Safety and Effectiveness of Adipose-Derived Stromal Cell vs Stromal Vascular Fraction Injection for Treatment of Knee Osteoarthritis: 2-Year Results of Parallel Single-Arm Trials2022

    • Author(s)
      Yokota Naomasa、Lyman Stephen、Hanai Hiroto、Shimomura Kazunori、Ando Wataru、Nakamura Norimasa
    • Journal Title

      The American Journal of Sports Medicine

      Volume: 50 Issue: 10 Pages: 2659-2668

    • DOI

      10.1177/03635465221107364

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 変形性関節症に対する治療効果を有する高純度脂肪由来間葉系間質細胞由来 細胞外小胞の回収法の確立2023

    • Author(s)
      花井洋人
    • Organizer
      第38回日本整形外科学会基礎学術集会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Extracellular vesicles with high purity derived from ASC cultured in a new medium suppresses progression of osteoarthritis in a mouse model2023

    • Author(s)
      花井洋人
    • Organizer
      17th ICRS World Congress 2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 間葉系幹細胞由来細胞外小胞(エクソソーム)を用いた変形性膝関節症治への臨床応用2023

    • Author(s)
      花井洋人
    • Organizer
      日本再生医療学会
    • Related Report
      2022 Research-status Report

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Published: 2022-09-01   Modified: 2025-01-30  

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