| Project/Area Number |
22K20968
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0906:Surgery related to the biological and sensory functions and related fields
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| Research Institution | Chiba University |
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Principal Investigator |
Akiba Ryutaro 千葉大学, 大学院医学研究院, 助教 (70963049)
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| Project Period (FY) |
2022-08-31 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2023: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 黄斑円孔 / 視細胞移植 / 細胞治療 / 電子顕微鏡 / 網膜再生 / ES細胞 / シナプス / 幹細胞 |
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| Outline of Research at the Start |
黄斑円孔は網膜中心部が障害され視力低下をきたす疾患であり、手術によって治療困難な症例が問題となっている。今回我々はニホンザルの黄斑円孔モデルに対し、ヒトES細胞由来の網膜組織を移植し、円孔閉鎖が可能であるかを検討する。さらに移植後の網膜組織をSerial Blockface Scanning Electron Microscopy (SBFSEM)で撮影し、得られた画像から網膜の神経細胞を三次元再構築およびシナプス解析を行うことで、移植した細胞とホストの網膜がシナプス形成を行い、網膜回路の修復が可能であるかを検討する。
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| Outline of Final Research Achievements |
Macular hole represents a condition in which damage to the central part of the retina leads to a reduction in visual acuity, with cases that are difficult to treat surgically posing a significant challenge. In collaboration with Riken, we transplanted human embryonic stem cell-derived retinal organoid into the eye of a Japanese Macaque with macular hole and subsequently dissected and fixed the retina, then captured images of the transplanted retinal tissue using electron microscopy. Analysis of these images revealed closure of the macular holes and the formation of rosettes by what are presumed to be host-derived photoreceptors. These findings suggest that transplantation of retinal tissue contributes to the closure of macular holes and can successfully integrate with the host retina. We plan to include these results in a paper to be submitted to an international academic journal.
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| Academic Significance and Societal Importance of the Research Achievements |
黄斑円孔の動物モデルにおいて、ヒトES細胞由来の網膜組織が円孔閉鎖に有用であることが明らかになり、今後細胞治療が黄斑円孔の治療に有用な可能性が示された。
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