Development of immunomodulatory scaffolds for tissue regeneration
| Project/Area Number |
22K20989
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0907:Oral science and related fields
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2022-08-31 – 2024-03-31
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| Project Status |
Granted (Fiscal Year 2022)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | Scaffolds / Macrophages / Immunomodulation / Biomaterials / Tissue regeneration |
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| Outline of Research at the Start |
It is hypothesized that regulation of macrophage activity can be achieved by using biomaterials. In this research project, the macrophage response to polymeric scaffolds developed in our laboratory will be evaluated.
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| Outline of Annual Research Achievements |
Our poly(lactic acid/caprolactone) copolymer (PLCL) technology was used to fabricate porous scaffolds of different sizes, from 200 um to 2.5 mm thickness. This achievement means that PLCL scaffolds can be adapted to numerous applications in tissue regeneration, and are not limited to bone regeneration as initially hypothesized in our proposal. Gas plasma treatment of PLCL scaffolds was performed, and new functional groups were detected by FTIR-spectroscopy. However, field emission SEM revealed different degrees of structural damage. Macrophages cultured on PLCL scaffolds showed a proliferation rate comparable to that of control materials; however, signs of scaffold degradation were observed by SEM, indicating that macrophages were engaged in the breakdown and phagocytosis of PLCL scaffold.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
We have established a modified method to fabricate PLCL porous scaffolds in various sizes. The PLCL scaffolds treated with gas plasma presented functional groups that can be used for loading bioactive molecules such as cytokines. Further evaluations of cytokine loaded PLCL can be performed within this fiscal year (2023). Overall, we believe that this research is progressing smoothly.
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| Strategy for Future Research Activity |
Considering the different degrees of damage to the PLCL scaffold structure observed by field emission SEM, we plan to further optimize the parameters for gas plasma treatment and minimize the damage. Additionally, plasma treated scaffolds will be loaded with a reference protein (bovine serum albumin) or with an immunomodulatory cytokine (interleukin 4), for analyzing their release profile. Finally, phagocytic activity and cytokine release of macrophages cultured on PLCL scaffolds will be evaluated.
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Report
(1 results)
Research Products
(2 results)
