| Project/Area Number |
22K20989
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0907:Oral science and related fields
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2022-08-31 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | Scaffolds / Biomaterials / Synthetic Polymers / Cells / Inflammation / Synthetic polymers / Macrophages / Immunomodulation / Tissue regeneration |
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| Outline of Research at the Start |
It is hypothesized that regulation of macrophage activity can be achieved by using biomaterials. In this research project, the macrophage response to polymeric scaffolds developed in our laboratory will be evaluated.
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| Outline of Final Research Achievements |
Poly(lactic acid/caprolactone) copolymer (PLCL) was used to fabricate a synthetic scaffold biomaterial. Physical and biological properties of PLCL scaffolds were assessed and compared with commercially available scaffolds. In vitro, human fibroblasts seeded onto the PLCL scaffolds proliferated and expressed higher levels of genes associated with wound healing in comparison with commercial scaffolds. PLCL scaffolds also showed biocompatibility and slow degradation in vivo, which is considered an advantage for treatments targeting bone regeneration. Therefore, an animal model of bone regeneration was implemented. Slow degrading PLCL scaffolds provided scaffold function for longer time when compared to collagen-derived scaffolds, resulting in significantly greater volume of regenerated bone. The results obtained in this study indicate that a PLCL scaffold is a useful and safe alternative to animal collagen-derived scaffolds.
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| Academic Significance and Societal Importance of the Research Achievements |
Scaffolds fabricated from synthetic polymers are essential biomaterials in dentistry. These biomaterials can replace animal collagen-derived scaffolds and contribute to tissue regeneration. The physical and biological properties of PLCL scaffolds make this material a novel treatment option.
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