| Project/Area Number |
22K21027
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0907:Oral science and related fields
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| Research Institution | Matsumoto Dental University |
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Principal Investigator |
Roy Rita Rani 松本歯科大学, 歯学部, 助教 (70963487)
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| Project Period (FY) |
2022-08-31 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | Swallowing Reflex / TRPV4 |
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| Outline of Research at the Start |
Dysphagia (Difficulty in swallowing) is a major health problem among aged population and patients with neurological diseases. Despite of its clinical significance, there has been no established pharmacological treatment for dysphagia. Understanding the functional involvement of TRPV4 in triggering swallowing reflex may help to develop pharmacological treatment strategy for the management of dysphagia.
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| Outline of Final Research Achievements |
We investigate the involvement of TRPV4 in initiating the swallowing reflex. Our observations reveal that TRPV4 immunoreactive nerve fibers present in the superior laryngeal nerve (SLN)-innervated swallowing-related regions. Retrograde tracing with fluorogold reveals localization of TRPV4 on approximately 25% of SLN-afferent neurons in the nodose-petrosal-jugular ganglionic complex. Among them, approximately 49% are large, 35% medium, and 15% small-sized SLN-afferent neurons. Furthermore, our investigation demonstrates that the topical administration of a chemical agonists targeting TRPV4, GSK1016790A, in swallowing-related regions, leads to a dose-dependent facilitation of the swallowing reflex. Notably, the preapplication of antagonists for TRPV4 significantly mitigates the GSK1016790A-induced swallowing reflex.
These findings underscore the potential of targeting TRPV4 to develop therapeutics to enhance swallowing function.
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| Academic Significance and Societal Importance of the Research Achievements |
Dysphagia poses a significant health challenge, with no established pharmacological treatment currently available. By targeting TRPV4, our study suggests the importance of further exploration and development of therapeutics to address this pressing health issue.
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