記憶メカニズムの多次元解析 - nmからメゾスケール/ミリ秒から日スケールまで

• Project/Area Number: 22K21353
• Research Category: Fund for the Promotion of Joint International Research (International Leading Research )
• Allocation Type: Multi-year Fund
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: 林 康紀 京都大学, 医学研究科, 教授 (90466037)
• Co-Investigator(Kenkyū-buntansha): 池谷 裕二 東京大学, 大学院薬学系研究科(薬学部), 教授 (10302613) ; 柚崎 通介 慶應義塾大学, 医学部(信濃町), 教授 (40365226) ; 久保 郁 国立遺伝学研究所, 遺伝形質研究系, 准教授 (40786373) ; 根本 知己 生理学研究所, 基盤神経科学研究領域, 教授 (50291084) ; 米原 圭祐 国立遺伝学研究所, 遺伝形質研究系, 教授 (80510619) ; 三國 貴康 新潟大学, 脳研究所, 教授 (90786477)
• Project Period (FY): 2022-12-20 – 2029-03-31
• Project Status: Granted (Fiscal Year 2024)
• Budget Amount: ¥689,000,000 (Direct Cost: ¥530,000,000、Indirect Cost: ¥159,000,000); Fiscal Year 2028: ¥91,000,000 (Direct Cost: ¥70,000,000、Indirect Cost: ¥21,000,000); Fiscal Year 2027: ¥93,600,000 (Direct Cost: ¥72,000,000、Indirect Cost: ¥21,600,000); Fiscal Year 2026: ¥93,600,000 (Direct Cost: ¥72,000,000、Indirect Cost: ¥21,600,000); Fiscal Year 2025: ¥93,600,000 (Direct Cost: ¥72,000,000、Indirect Cost: ¥21,600,000); Fiscal Year 2024: ¥93,600,000 (Direct Cost: ¥72,000,000、Indirect Cost: ¥21,600,000); Fiscal Year 2023: ¥93,600,000 (Direct Cost: ¥72,000,000、Indirect Cost: ¥21,600,000); Fiscal Year 2022: ¥130,000,000 (Direct Cost: ¥100,000,000、Indirect Cost: ¥30,000,000)
• Keywords: 記憶 / シナプス可塑性 / 神経回路

Outline of Research at the Start

7研究室は分子解析チーム（林、柚﨑）、回路・行動解析チーム（池谷、久保、三國、米原）、技術開発チーム（根本）の3つに分かれる。分子解析チームはシナプス可塑性のナノスケールの分子機構に主眼を置く一方、新しい分子解析技術を回路・行動解析チームに提供する。神経回路・行動解析チームは、記憶に伴う神経回路の再構成をシナプス可塑性に重点を置き解析していく一方、分子解析チームに対して有用なプローブや操作技術などのアイデアを出す。このように3つのチームがそれぞれの研究を進める一方、国際先導研究の枠組みを活かして、国内拠点および海外拠点と相互交流することによって、新しい技術開発と研究を強力に推進する。

Outline of Annual Research Achievements

2024年度は、長期留学が池谷研究室からマックスプランク研究所の安田先生の研究室に1名希望者（博士後期課程学生）があり、2023年から継続でボルドー大学出身で京都大学の林の研究室に来ている者（ポスドク）と合わせて、2名となった。また、短期派遣として、ボルドー大学のイメージングコースに3名を派遣した。その内訳は池谷研究室、柚崎研究室、米原研究室各1名である。同様にマックスプランク研究所のイメージングコースにも久保研究室、池谷研究室から各1名の2名を派遣した。いずれのコースも講義と研究室配属に参加した。
一方、9月にはマックスプランク研究所の安田先生の主催で第2回リトリートを行い、日本からPIが5名、PIでないスタッフが1名、若手研究者が11名参加した。マックスプランク側の参加者も合わせて20講演、またポスター発表も用意された。若手研究者はシンポジウム後、研究室を訪問し、実習をおこなった。
さらにボルドー大学(最近、ゲッティンゲン大学へ異動)のPIであるValentin Naegerl先生に来日を乞い、参加している7研究室のうち、一部を回っていただいた。それぞれの研究室では、セミナー、PIおよび大学院生、若手スタッフとの交流を行った。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

長期留学生、短期訪問の実績が上がっているため。

Strategy for Future Research Activity

本年度も同様に短期、長期の留学生を募る。またボルドー大学にてシンポジウムと若手のラボ見学を行う。

Research Products

• [Int'l Joint Research] ボルドー大学(フランス)
• [Int'l Joint Research] マックスプランク研究所(米国)
• [Journal Article] Time-window of offline long-term potentiation in anterior cingulate cortex during memory consolidation and recall (2025)
  • Author(s): Liu Junyu、Goto Akihiro、Hayashi Yasunori
  • Journal Title: Neuroscience Research Volume: 212 Pages: 75-83
  • DOI: 10.1016/j.neures.2024.12.009
  • Peer Reviewed / Open Access
• [Journal Article] Operant House: A Versatile Open-Source Platform for Automated Operant Conditioning Testing of Mice in Home Cages (2025)
  • Author(s): Otsuka Shintaro、Nakamura Yuriko、Ito-Ishida Aya、Suzuki Kunimichi、Ishikawa Ayako、Matsuda Keiko、Suyama Shigetomo、Contractor Anis、Yuzaki Michisuke
  • Journal Title: bioRxiv Volume: -
  • DOI: 10.1101/2025.01.24.634815
  • Open Access
• [Journal Article] Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia (2025)
  • Author(s): Andoh Megumi、Shinoda Natsuki、Taira Yusuke、Araki Tasuku、Kasahara Yuka、Takeuchi Haruki、Miura Masayuki、Ikegaya Yuji、Koyama Ryuta
  • Journal Title: Nature Communications Volume: 16 Issue: 1 Pages: 918-918
  • DOI: 10.1038/s41467-025-56342-7
  • Peer Reviewed / Open Access
• [Journal Article] Motion detection: Specific thalamocortical connections revealed (2025)
  • Author(s): Matsumoto Akihiro、Yonehara Keisuke
  • Journal Title: Current Biology Volume: 35 Issue: 1 Pages: R11-R13
  • DOI: 10.1016/j.cub.2024.11.019
  • Peer Reviewed
• [Journal Article] Cellular Responses to Photothermal Therapy: Heat-Induced ERK Signaling and Intercellular Communication in Solid Tumors (2025)
  • Author(s): Taemaitree Farsai、Takano Yuta、Yamaguchi Daisuke、Yamaguchi Kazushi、Yamashita Yudai、Tsutsumi Motosuke、Wen Chentao、Otomo Kohei、Kenji Hirai、James Hutchison、Indra Van Zundert、Sandra Krzyzowska、Maria Bravo、Sayuki Hirano、Koutarou D Kimura、Kazuhiro Aoki、Susana Rocha、Tomomi Nemoto、Beatrice Fortuni et al
  • Journal Title: bioRxiv Volume: -
  • DOI: 10.1101/2025.02.27.640541
  • Open Access / Int'l Joint Research
• [Journal Article] Multiphasic protein condensation governed by shape and valency (2025)
  • Author(s): Pandey Vikas、Hosokawa Tomohisa、Hayashi Yasunori、Urakubo Hidetoshi
  • Journal Title: Cell Reports Volume: 44 Issue: 4 Pages: 115504-115504
  • DOI: 10.1016/j.celrep.2025.115504
  • Peer Reviewed / Open Access
• [Journal Article] Abundant extrasynaptic expression of α3β4-containing nicotinic acetylcholine receptors in the medial habenula-interpeduncular nucleus pathway in mice (2024)
  • Author(s): Tsuzuki Asuka、Yamasaki Miwako、Konno Kohtarou、Miyazaki Taisuke、Takei Norio、Tomita Susumu、Yuzaki Michisuke、Watanabe Masahiko
  • Journal Title: Scientific Reports Volume: 14 Issue: 1 Pages: 14193-14193
  • DOI: 10.1038/s41598-024-65076-3
  • Peer Reviewed / Open Access
• [Journal Article] Kainate receptors regulate synaptic integrity and plasticity by forming a complex with synaptic organizers in the cerebellum (2024)
  • Author(s): Kakegawa Wataru、Paternain Ana V.、Matsuda Keiko、Aller M. Isabel、Iida Izumi、Miura Eriko、Nozawa Kazuya、Yamasaki Tokiwa、Sakimura Kenji、Yuzaki Michisuke、Lerma Juan
  • Journal Title: Cell Reports Volume: 43 Issue: 7 Pages: 114427-114427
  • DOI: 10.1016/j.celrep.2024.114427
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Lack of evidence for direct ligand-gated ion channel activity of GluD receptors (2024)
  • Author(s): Itoh Masayuki、Piot Laura、Mony Laetitia、Paoletti Pierre、Yuzaki Michisuke
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 121 Issue: 31 Pages: 31-31
  • DOI: 10.1073/pnas.2406655121
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A fluorophore-ligand conjugate for the rapid and reversible staining of native AMPA receptors in living neurons (2024)
  • Author(s): Soga Kyohei、Fujiwara Takaaki、Nakagawa Mayu、Shibata Akihiro、Adriel Hansel、Yatsuzuka Kenji、Kakegawa Wataru、Yuzaki Michisuke、Hamachi Itaru、Nango Eriko、Kiyonaka Shigeki
  • Journal Title: bioRxiv Volume: -
  • DOI: 10.1101/2024.08.21.608930
  • Open Access
• [Journal Article] Macrophages and nociceptor neurons form a sentinel unit around fenestrated capillaries to defend the synovium from circulating immune challenge (2024)
  • Author(s): Hasegawa Tetsuo、Lee Colin Y. C.、Hotchen Andrew J.、Fleming Aaron、Singh Rahul、Suzuki Kunimichi、Yuzaki Michisuke、Watanabe Masahiko、Birch Mark A.、McCaskie Andrew W.、Lenart Nikolett、Toth Krisztina、Denes Adam、Liu Zhaoyuan、Ginhoux Florent、Richoz Nathan、Clatworthy Menna R.
  • Journal Title: Nature Immunology Volume: 25 Issue: 12 Pages: 2270-2283
  • DOI: 10.1038/s41590-024-02011-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A method to analyze gene expression profiles from hippocampal neurons electrophysiologically recorded in vivo. (2024)
  • Author(s): Yagishita H, Go Y, Okamoto K, Arimura N, Ikegaya Y, Sasaki T.
  • Journal Title: Front Neurosci. Volume: 18 Pages: 1360432-1360432
  • DOI: 10.3389/fnins.2024.1360432
  • Peer Reviewed / Open Access
• [Journal Article] The meaning of boredom: Properly managing childhood boredom could lead to more fulfilling lives. (2024)
  • Author(s): Uehara, I., & Ikegaya, Y.
  • Journal Title: EMBO reports Volume: in press Issue: 6 Pages: 2515-2519
  • DOI: 10.1038/s44319-024-00155-0
  • Peer Reviewed / Open Access
• [Journal Article] Top-down brain circuits for operant bradycardia (2024)
  • Author(s): Yoshimoto Airi、Morikawa Shota、Kato Eriko、Takeuchi Haruki、Ikegaya Yuji
  • Journal Title: Science Volume: 384 Issue: 6702 Pages: 1361-1368
  • DOI: 10.1126/science.adl3353
  • Peer Reviewed
• [Journal Article] Optogenetic estimation of synaptic connections in brain slices (2024)
  • Author(s): Kashima Tetsuhiko、Sasaki Takuya、Ikegaya Yuji
  • Journal Title: Journal of Neuroscience Methods Volume: 412 Pages: 110298-110298
  • DOI: 10.1016/j.jneumeth.2024.110298
  • Peer Reviewed / Open Access
• [Journal Article] Single-cell synaptome mapping: its technical basis and applications in critical period plasticity research (2024)
  • Author(s): Uchigashima Motokazu、Mikuni Takayasu
  • Journal Title: Frontiers in Neural Circuits Volume: 18 Pages: 1523614-1523614
  • DOI: 10.3389/fncir.2024.1523614
  • Peer Reviewed / Open Access
• [Journal Article] Signal Improved ultra-Fast Light-sheet Microscope (SIFT) for large tissue imaging (2024)
  • Author(s): Md Nasful Huda Prince、Benjamin Garcia、Cory Henn、Yating Yi、Etsuo A Susaki、Yuki Watakabe、Tomomi Nemoto、Keith A Lidke、Hu Zhao、Irene Salinas、Sheng Liu、Tonmoy Chakraborty
  • Journal Title: Communications Engineering Volume: 3 Issue: 1
  • DOI: 10.1038/s44172-024-00205-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Live imaging of center of calcification formation during septum development in primary polyps of Acropora digitifera (2024)
  • Author(s): Ohno Yoshikazu、Takahashi Anan、Tsutsumi Motosuke、Kubota Azusa、Iguchi Akira、Iijima Mariko、Mizusawa Nanami、Nakamura Takashi、Suzuki Atsushi、Suzuki Michio、Yasumoto Jun、Watabe Shugo、Sakai Kazuhiko、Nemoto Tomomi、Yasumoto Ko
  • Journal Title: Frontiers in Marine Science Volume: 11 Pages: 1-13
  • DOI: 10.3389/fmars.2024.1406446
  • Peer Reviewed / Open Access
• [Journal Article] descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques (2024)
  • Author(s): Otomo Kohei、Omura Takaki、Kaku Masaru、Susaki Etsuo A. et al.
  • Journal Title: Nature Communications Volume: 15 Issue: 1 Pages: 4941-4941
  • DOI: 10.1038/s41467-024-49131-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Amyloid-β-induced Alteration of Fast and Localized Calcium Elevations in Cultured Astrocytes (2024)
  • Author(s): Nakata Kaito、Sakamoto Joe、Otomo Kohei、Sato Masanao、Ishii Hirokazu、Tsutsumi Motosuke、Enoki Ryosuke、Nemoto Tomomi
  • Journal Title: bioRxiv Volume: -
  • DOI: 10.1101/2024.09.23.614604
  • Open Access
• [Journal Article] 4D Evaluation Approach for Cell Signaling Activity in Multicellular Tumor Spheroids (2024)
  • Author(s): Tsutsumi Motosuke、Yamaguchi Kazushi、Nemoto Tomomi
  • Journal Title: Nanomedicine and Nanotoxicology Volume: - Pages: 293-307
  • DOI: 10.1007/978-981-97-5288-1_15
  • ISBN: 9789819752874, 9789819752881
  • Peer Reviewed
• [Journal Article] Absorption, Fluorescence, and Two‐Photon Excitation Ability of 5‐o‐Tolyl‐11 (or 13)‐<i>o</i>‐tolylisoindolo[2,1‐<i>a</i>]quinolines Prepared by Ring‐Closing Metathesis and [2+3] Cycloaddition (2024)
  • Author(s): Wada Yuki、Jang Kwangkyun、Ishii Hirokazu、Watakabe Yuki、Tsutsumi Motosuke、Sako Makoto、Takehara Tsunayoshi、Suzuki Takeyuki、Tsujino Hirofumi、Tsutsumi Yasuo、Nemoto Tomomi、Arisawa Mitsuhiro
  • Journal Title: Chemistry An Asian Journal Volume: 20 Issue: 2
  • DOI: 10.1002/asia.202401073
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Transport-of-intensity phase imaging using commercially available confocal microscope (2024)
  • Author(s): Yoneda Naru、Sakamoto Joe、Tomoi Takumi、Nemoto Tomomi、Tamada Yosuke、Matoba Osamu
  • Journal Title: Journal of Biomedical Optics Volume: 29 Issue: 11 Pages: 116002-116002
  • DOI: 10.1117/1.jbo.29.11.116002
  • Peer Reviewed / Open Access
• [Journal Article] Cell Division Imaging of Tobacco BY-2 Cells in a 3D Volume by Two-Photon Spinning-Disk Confocal Microscopy (2024)
  • Author(s): Murata Takashi、Tsutsumi Motosuke、Otomo Kohei、Nemoto Tomomi
  • Journal Title: Methods in Molecular Biology Volume: 2872 Pages: 37-50
  • DOI: 10.1007/978-1-0716-4224-5_3
  • ISBN: 9781071642238, 9781071642245
  • Peer Reviewed
• [Journal Article] ２光子超解像顕微鏡法の開発 (2024)
  • Author(s): 堤 元佐, 石井宏和, 根本知己
  • Journal Title: BRAIN and NERVE Volume: 76(7) Pages: 807-812
• [Journal Article] 生体3Dイメージングを実現する蛍光顕微鏡技術―より広く、深く、精密に (2024)
  • Author(s): 大友康平, 高橋泰伽, 石井宏和, 根本知己, 洲崎悦生
  • Journal Title: 実験医学 Volume: 42(19) Pages: 2961-2967
• [Journal Article] ナノマテリアルを活用したマウス脳の広範囲・深部in vivo光イメージング法の開発 (2024)
  • Author(s): 高橋泰伽 堤香琳 曽我公平 榎木亮介 岡村陽介 根本知己
  • Journal Title: バイオイメージング Volume: 33(2) Pages: 51-51
• [Journal Article] 冬眠様状態マウスの海馬CA1領域における樹状突起スパイン形態のin vivo二光子イメージング (2024)
  • Author(s): 堤香琳, 榎木亮介, 曽我公平, 根本知己, 高橋泰伽
  • Journal Title: バイオイメージング Volume: 33(2) Pages: 91-91
• [Journal Article] 学術研究支援の最先端 I.先端バイオイメージング支援プラットフォーム(ABiS)i.光学顕微鏡支援 ナノ材料を用いたマウス生体脳の超広範囲イメージング (2024)
  • Author(s): 高橋泰伽, 岡村陽介, 根本知己
  • Journal Title: 生体の科学 Volume: 75(5) Pages: 398-399
• [Journal Article] Imaging of Structural Plasticity of Dendritic Spines with Two-Photon Microscopy (2024)
  • Author(s): Saneyoshi Takeo、Hayashi Yasunori
  • Journal Title: Methods Mol Biol. Volume: 2831 Pages: 209-217
  • DOI: 10.1007/978-1-0716-3969-6_14
  • ISBN: 9781071639689, 9781071639696
  • Peer Reviewed
• [Journal Article] 時空間蛍光相関解析による生体深部2光子超解像イメージングの実現 (2024)
  • Author(s): 堤元佐, 高橋泰伽, 小林健太郎, 根本知己
  • Journal Title: バイオイメージング Volume: 33(2) Pages: 68-68
• [Journal Article] Multibeam continuous axial scanning two-photon microscopy for in vivo volumetric imaging in mouse brain (2024)
  • Author(s): Ataka Mitsutoshi、Otomo Kohei、Enoki Ryosuke、Ishii Hirokazu、Tsutsumi Motosuke、Kozawa Yuichi、Sato Shunichi、Nemoto Tomomi
  • Journal Title: Biomedical Optics Express Volume: 15 Issue: 2 Pages: 1089-1089
  • DOI: 10.1364/boe.514826
  • Peer Reviewed / Open Access
• [Journal Article] Actin dynamics switches two distinct modes of endosomal fusion in yolk sac visceral endoderm cells (2024)
  • Author(s): Seiichi Koike、Masashi Tachikawa、Motosuke Tsutsumi、Takuya Okada、Tomomi Nemoto、Kazuko Keino-Masu、Masayuki Masu
  • Journal Title: eLife Volume: 13
  • DOI: 10.7554/elife.95999.1
  • Peer Reviewed / Open Access
• [Journal Article] Large-scale cranial window for in vivo mouse brain imaging utilizing fluoropolymer nanosheet and light-curable resin (2024)
  • Author(s): Takahashi Taiga、Zhang Hong、Agetsuma Masakazu、Nabekura Junichi、Otomo Kohei、Okamura Yosuke、Nemoto Tomomi
  • Journal Title: Communications Biology Volume: 7 Issue: 1 Pages: 232-232
  • DOI: 10.1038/s42003-024-05865-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The hidden face of GluD1 at inhibitory synapses (2024)
  • Author(s): Itoh Masayuki、Yuzaki Michisuke
  • Journal Title: Cell Research Volume: 121 Issue: 6 Pages: 1-2
  • DOI: 10.1038/s41422-024-00931-6
  • Open Access
• [Journal Article] Bioorthogonal chemical labeling of endogenous neurotransmitter receptors in living mouse brains (2024)
  • Author(s): Nonaka Hiroshi、Sakamoto Seiji、Shiraiwa Kazuki、Ishikawa Mamoru、Tamura Tomonori、Okuno Kyohei、Kondo Takumi、Kiyonaka Shigeki、Susaki Etsuo A.、Shimizu Chika、Ueda Hiroki R.、Kakegawa Wataru、Arai Itaru、Yuzaki Michisuke、Hamachi Itaru
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 121 Issue: 6 Pages: 2313887121-2313887121
  • DOI: 10.1073/pnas.2313887121
  • Peer Reviewed / Open Access
• [Journal Article] Neuronal DSCAM regulates the peri-synaptic localization of GLAST in Bergmann glia for functional synapse formation (2024)
  • Author(s): Dewa Ken-ichi、Arimura Nariko、Kakegawa Wataru et al.
  • Journal Title: Nature Communications Volume: 15 Issue: 1 Pages: 458-458
  • DOI: 10.1038/s41467-023-44579-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 光学顕微鏡を用いた生体蛍光イメージング技術の進歩 (2023)
  • Author(s): 坂本丞、石井宏和、根本知己
  • Journal Title: 週刊「医学のあゆみ」 Volume: 286 (5) Pages: 3124-317
• [Journal Article] All-synchronized picosecond pulses and time-gated detection improve the spatial resolution of two-photon STED microscopy in brain tissue imaging (2023)
  • Author(s): Hirokazu Ishii、Kohei Otomo、Ching-Pu Chang、Miwako Yamasaki、Masahiko Watanabe、Hiroyuki Yokoyama、Tomomi Nemoto
  • Journal Title: PLOS ONE Volume: 18(8) Issue: 8 Pages: 7462-7467
  • DOI: 10.1371/journal.pone.0290550
  • Peer Reviewed / Open Access
• [Journal Article] Fluorescence radial fluctuation enables two-photon super-resolution microscopy (2023)
  • Author(s): Tsutsumi Motosuke、Takahashi Taiga、Kobayashi Kentaro、Nemoto Tomomi
  • Journal Title: Frontiers in Cellular Neuroscience Volume: 17
  • DOI: 10.3389/fncel.2023.1243633
  • Peer Reviewed / Open Access
• [Journal Article] 生体組織・細胞を美しくイメージングするための高分子ナノ薄膜 (2023)
  • Author(s): 岡村陽介、高橋泰伽、根本知己
  • Journal Title: バイオマテリアル Volume: 41(4) Pages: 284-289
• [Journal Article] Cold-induced Suspension and Resetting of Ca2+ and Transcriptional Rhythms in the Suprachiasmatic Nucleus Neurons. (2023)
  • Author(s): Ryosuke Enoki、Naohiro Kon、Kimiko Shimizu、Kenta Kobayashi、Sota Hiro、Ching-Pu Chang、Tatsuto Nakane、Hirokazu Ishii、Joe Sakamoto、Yoshifumi Yamaguchi、Tomomi Nemoto
  • Journal Title: iScience Volume: 26(12) Issue: 12 Pages: 108390-108390
  • DOI: 10.1016/j.isci.2023.108390
  • Peer Reviewed / Open Access
• [Journal Article] 極低温下における概日時計中枢のリズム発振機構 (2023)
  • Author(s): 榎木亮介、根本知己
  • Journal Title: 月刊「細胞」 Volume: 55(14) Pages: 32-35
• [Journal Article] In-phasic cytosolic-nuclear Ca2+ rhythms in suprachiasmatic nucleus neurons (2023)
  • Author(s): Sota Hiro、Kenta Kobayashi、Tomomi Nemoto、Ryosuke Enoki
  • Journal Title: Frontiers in Neuroscience Volume: 17
  • DOI: 10.3389/fnins.2023.1323565
  • Peer Reviewed / Open Access
• [Journal Article] Emerging computational motifs: Lessons from the retina (2023)
  • Author(s): Matsumoto Akihiro、Yonehara Keisuke
  • Journal Title: Neuroscience Research Volume: S0168～0102 Pages: 00108-6
  • DOI: 10.1016/j.neures.2023.06.003
  • Peer Reviewed
• [Journal Article] Recombinase-independent AAV for anterograde transsynaptic tracing (2023)
  • Author(s): Faress Islam、Khalil Valentina、Yamamoto Haruka、Sajgo Szilard、Yonehara Keisuke、Nabavi Sadegh
  • Journal Title: Molecular Brain Volume: 16 Issue: 1 Pages: 66-66
  • DOI: 10.1186/s13041-023-01053-7
• [Journal Article] Subcortico-amygdala pathway processes innate and learned threats (2023)
  • Author(s): Khalil Valentina、Faress Islam、Mermet-Joret No?mie、Kerwin Peter、Yonehara Keisuke、Nabavi Sadegh
  • Journal Title: eLife Volume: 12
  • DOI: 10.7554/elife.85459
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] C1ql1-Bai3 signaling is necessary for climbing fiber synapse formation in mature Purkinje cells in coordination with neuronal activity (2023)
  • Author(s): Aimi Takahiro、Matsuda Keiko、Yuzaki Michisuke
  • Journal Title: Molecular Brain Volume: 16 Issue: 1 Pages: 61-61
  • DOI: 10.1186/s13041-023-01048-4
  • Peer Reviewed / Open Access
• [Journal Article] Advanced observation of brain and nerve cells using two-photon microscopy with novel techniques (2022)
  • Author(s): Ishii Hirokazu、Takahashi Taiga、Yamaguchi Kazushi、Nemoto Tomomi
  • Journal Title: Microscopy Volume: 72 Issue: 2 Pages: 144-150
  • DOI: 10.1093/jmicro/dfac047
  • Peer Reviewed / Open Access
• [Presentation] Abstractive representation of spatial context memory in the anterior cingulate gyrus (2025)
  • Author(s): Yasunori Hayashi
  • Organizer: Molecular and cellular cognition society
  • Int'l Joint Research / Invited
• [Presentation] 皮質視床ネットワークにおけるシータリズムを介した心拍数調節 (2025)
  • Author(s): 吉本愛梨
  • Organizer: 生理研研究会発達脳2024
• [Presentation] 同期発火は機能的なシナプス結合形成を促進する (2025)
  • Author(s): 鹿島哲彦
  • Organizer: 生理研研究会発達脳2024
• [Presentation] 脳回路の機能拡張に関する探索的研究 (2025)
  • Author(s): 池谷裕二
  • Organizer: 日本薬学会 第145回年会
• [Presentation] 冬眠様状態マウスにおける感覚刺激に対する大脳皮質応答の生体内2光子カルシウムイメージング (2025)
  • Author(s): Chang Ching-Pu, Lee Ming-Liang, Nemoto Tomomi, Enoki Ryosuke
  • Organizer: APPW2025
• [Presentation] Body temperature regulates glucose metabolism and torpid behavior (2025)
  • Author(s): Lee Ming-Liang, Chang Ching-Pu, Toda Chitoku, Nemoto Tomomi, Enoki Ryosuke
  • Organizer: APPW2025
  • Invited
• [Presentation] Tunable-wavelength STED Laser-integrated Two-photon Microscopyfor In Vivo Mouse Brain Imaging (2025)
  • Author(s): Joe Sakamoto, Hirokazu Ishii, Tomomi Nemoto
  • Organizer: 第14回生理研・脳研・ヒト進化研究センター合同シンポジウム
• [Presentation] Reconstruction of Optical Turbulence Toward Quantitative Temperature Measurement in the Living Cells (2025)
  • Author(s): Joe Sakamoto, Masaki Watabe
  • Organizer: 第7回ExCELLSシンポジウム
• [Presentation] Intracellular thermometry toward reconstruction of the temperature distribution in the living cells (2025)
  • Author(s): Joe Sakamoto
  • Organizer: 第7回ExCELLSシンポジウム
• [Presentation] Online- and offline-LTP underlying memory consolidation (2024)
  • Author(s): Yasunori Hayashi
  • Organizer: Chineae Neuroscience Society
  • Int'l Joint Research / Invited
• [Presentation] Online- and offline-LTP underlying memory consolidation (2024)
  • Author(s): Yasunori Hayashi
  • Organizer: US-Japan Brain Seminar
  • Int'l Joint Research / Invited
• [Presentation] Functional and morphological characterization of zebrafish retinal ganglion cell subtypes expressing the transcription factor Satb2 (2024)
  • Author(s): Ayjan Urazbayeva
  • Organizer: 18th International Zebrafish Conference
  • Int'l Joint Research
• [Presentation] Molecular targeting and functional analysis of neuronal subtypes of the optic flow processing circuit in larval zebrafish (2024)
  • Author(s): 久保 郁
  • Organizer: Neuro2024シンポジウム
• [Presentation] 小型脊椎動物モデルを用いた視覚研究 (2024)
  • Author(s): 久保 郁
  • Organizer: Neuro2024教育講演
  • Invited
• [Presentation] ゼブラフィッシュにおける神経ネットワーク解析：大規模神経活動イメージングとそれに直交するアプローチ (2024)
  • Author(s): 久保 郁
  • Organizer: 生理研研究会「大規模脳活動計測 我々は何を測り、どこへ行くのか？」
  • Invited
• [Presentation] 視覚の情報処理と行動を支える神経回路 (2024)
  • Author(s): 久保 郁
  • Organizer: 京都大学生命科学研究科創立25周年記念シンポジウム
  • Invited
• [Presentation] Neural network analysis using activity-based cell labeling and transcriptome analysis in zebrafish (2024)
  • Author(s): 久保 郁
  • Organizer: 第97回日本生化学会大会
  • Invited
• [Presentation] Single-cell synaptome mapping of spatiotemporally different protein subpopulations in the brain (2024)
  • Author(s): Takayasu Mikuni
  • Organizer: NEURO2024
• [Presentation] Emergence of functional retinal circuits during postnatal development (2024)
  • Author(s): Keisuke Yonehara
  • Organizer: SPONT2024
  • Int'l Joint Research
• [Presentation] Emergence of functional retinal circuits during postnatal development (2024)
  • Author(s): Keisuke Yonehara
  • Organizer: Retreat for Multidimensional Analysis of Memory Mechanisms 2024
  • Int'l Joint Research / Invited
• [Presentation] Emergence of retinal motion detection circuits during neonatal development (2024)
  • Author(s): Keisuke Yonehara
  • Organizer: Kick-off Symposium for Transformative Research Area(A) Emergence of Brain Functions from the Dynamic Connectome
• [Presentation] Diverse GABA signaling in the inner retina enables spatiotemporal coding (2024)
  • Author(s): Keisuke Yonehara
  • Organizer: Invited seminar at Vision Institute, Paris
  • Int'l Joint Research
• [Presentation] Diverse neural activity in the mouse superior colliculus (2024)
  • Author(s): KEISUKE YONEHARA
  • Organizer: Invited seminar at IINS, Bordeaux Neurocampus
  • Int'l Joint Research / Invited
• [Presentation] Diverse neural activity in the mouse superior colliculus (2024)
  • Author(s): KEISUKE YONEHARA
  • Organizer: Invited seminar at UCSF Dept. of Physiology
  • Int'l Joint Research / Invited
• [Presentation] 新生仔期の自発神経活動が創発する 網膜の動き検出回路 (2024)
  • Author(s): 米原圭祐
  • Organizer: 学術変革領域研究（A）領域会議
  • Invited
• [Presentation] Advancements in in vivo Two-Photon Microscopic Imaging in the Mouse Brain through Novel Optical Technologies (2024)
  • Author(s): Tomomi Nemoto
  • Organizer: Optics & Photonics Taiwan International Conference (OPIC2024)
  • Int'l Joint Research / Invited
• [Presentation] Multi-photon Microscopy Enhanced by Manipulation of Excitation Laser Beam and its Application to Cellular Physiology (2024)
  • Author(s): Tomomi Nemoto
  • Organizer: Biomedical imaging and sensing conference in OPTICS & PHOTONICS International Congress 2024 (OPITC BISC),
  • Int'l Joint Research / Invited
• [Presentation] Multi-photon microscopy advanced by novel optical technologies (2024)
  • Author(s): Tomomi Nemoto
  • Organizer: 2024 Yonsei University-Korea University-NIPS Symposium
  • Int'l Joint Research / Invited
• [Presentation] Two-photon microscopy advanced by novel technologies (2024)
  • Author(s): Tomomi Nemoto
  • Organizer: Frontier Bioorganization Forum 2024
  • Invited
• [Presentation] 多光子過程に基づいた顕微鏡法の原理と応用 (2024)
  • Author(s): 根本知己
  • Organizer: 第47回日本神経科学大会
• [Presentation] All-pulsed two-photon STED microscopy for nanoscale tissue imaging (2024)
  • Author(s): Hirokazu Ishii, Kohei Otomo, Joe Sakamoto, Tomomi Nemoto
  • Organizer: LDC OPIC2024
• [Presentation] 心拍数を意図的にコントロールするTop-Down神経回路 (2024)
  • Author(s): 吉本愛梨
  • Organizer: 生理研研究会情動研究会2024
• [Presentation] 超初心者向け解説：シナプス可塑性モデルとしての海馬 (2024)
  • Author(s): 池谷裕二
  • Organizer: 第30回海馬と高次脳機能学会
• [Presentation] Clock and Metabolism under Extreme Cold (2024)
  • Author(s): Ryosuke Enoki
  • Organizer: 国際先導リトリート:マックスプランクフロリダ神経科学研究所
  • Int'l Joint Research / Invited
• [Presentation] Body temperature regulates glucose metabolism and torpid behavior (2024)
  • Author(s): Lee Ming-Liang, Chang Ching-Pu, Toda Chitoku, Nemoto Tomomi, Enoki Ryosuke
  • Organizer: Federation of European Neuroscience Societies (FENS) Forum 2024
  • Int'l Joint Research
• [Presentation] In vivo two-photon calcium imaging of cortical activity during a hibernation-like state in mice (2024)
  • Author(s): Chang Ching-Pu, Lee Ming-Liang, Nemoto Tomomi, Enoki Ryosuke
  • Organizer: Federation of European Neuroscience Societies (FENS) Forum 2024
  • Int'l Joint Research
• [Presentation] Body temperature regulates glucose metabolism and torpid behavior (2024)
  • Author(s): Lee Ming-Liang, Chan Ching-Pu, Toda Chitoku, Nemoto Tomomi, Enoki Ryosuke
  • Organizer: 17th International Hibernation Symposium
  • Int'l Joint Research
• [Presentation] In vivo two-photon calcium imaging of cortical activity during a hibernation-like state in mice (2024)
  • Author(s): Chang Ching-Pu, Lee Ming-Liang, Nemoto Tomomi, Enoki Ryosuke
  • Organizer: 17th International Hibernation Symposium
  • Int'l Joint Research
• [Presentation] Body temperature regulates glucose metabolism and torpid behavior (2024)
  • Author(s): Lee Ming-Liang, Chan Ching-Pu, Nemoto Tomomi, Enoki Ryosuke
  • Organizer: 2024 YONSEI UNIV. KOREA UNIV. NIPS SYMPOSIUM
  • Int'l Joint Research / Invited
• [Presentation] Development of wavelength-optimized two-photon pulsed STED nanoscopy utilizing supercontinuum light source (2024)
  • Author(s): Joe Sakamoto, Hirokazu Ishii, Tomomi Nemoto
  • Organizer: 国際先導リトリート：マックスプランクフロリダ神経科学研究所
  • Int'l Joint Research
• [Presentation] 超解像生体脳イメージングに向けた2 光子波長可変 STED 顕微鏡の開発 (2024)
  • Author(s): 坂本丞, 石井宏和, 根本知己
  • Organizer: 第71回中部日本生理学会
• [Presentation] 定量位相イメージングによる細胞内光学乱流の計測と細胞内温度計測への展開 (2024)
  • Author(s): 坂本丞, 米田成, 渡部匡己
  • Organizer: Biothermology Workshop 2024
• [Presentation] In vivo volumetric imaging in mouse brain using multibeam scanning two-photon microscopy (2024)
  • Author(s): Mitsutoshi Ataka、Kohei Otomo、Tomomi Nemoto
  • Organizer: 国際先導リトリート：マックスプランクフロリダ神経科学研究所
  • Int'l Joint Research
• [Presentation] All-pulsed two-photon STED microscopy for brain tissue nanoimaging (2024)
  • Author(s): Hirokazu Ishii、Joe Sakamoto、Kohei Otomo、Tomomi Nemoto
  • Organizer: Focus On Microscopy 2024
  • Int'l Joint Research
• [Presentation] 多光子過程を用いた細胞機能の顕微可視化解析の原理と展開 (2024)
  • Author(s): 根本知己
  • Organizer: 東北大学光メタセンシング共創研究所講演会
  • Invited
• [Presentation] 時空間蛍光相関解析による２光子深部超解像イメージング (2024)
  • Author(s): 堤 元佐、_橋泰伽、小林健太郎、根本知己
  • Organizer: 第６回ExCELLSシンポジウム
• [Presentation] 先端光技術を用いたイメージングでみえてきたもの (2024)
  • Author(s): 根本知己
  • Organizer: 先端光技術を基軸としたバイオイメージング研究会
  • Invited
• [Presentation] 高分子ナノシートを活用したマウス脳の広範囲in vivo二光子イメージング技術の開発 (2023)
  • Author(s): 高橋泰伽、張宏、揚妻正和、鍋倉淳一、大友康平、岡村陽介、根本知己
  • Organizer: AMED精神・神経疾患領域/早期ライフ連携推進Workshop
• [Presentation] In vivo two-photon imaging through a large-scale cranial window for mouse brain utilizing fluoropolymer nanosheet and light-curable resin (2023)
  • Author(s): 高橋泰伽、張宏、揚妻正和、鍋倉淳一、大友康平、岡村陽介、根本知己
  • Organizer: 第66回日本神経化学会大会
• [Presentation] 超解像二光子イメージング法による オルガネラ可視化 (2023)
  • Author(s): 石井宏和、根本知己
  • Organizer: ExCELLS先端共創プラットフォーム「オルガネラの時空間アトラス編纂」キックオフミーティング
• [Presentation] Long-term imaging of organellar rhythms in the suprachiasmatic nucleus neurons (2023)
  • Author(s): Sota Hiro他
  • Organizer: Gordon research coference
  • Int'l Joint Research
• [Presentation] Cold-induced Suspension and Resetting of Ca2+ and Transcriptional Rhythms in the Suprachiasmatic Nucleus Neurons (2023)
  • Author(s): Ryosuke Enoki他
  • Organizer: Gordon Conference
  • Int'l Joint Research
• [Presentation] 先端光技術を用いたバイオイメージングの展開 (2023)
  • Author(s): 根本知己
  • Organizer: 第34回生理科学実験技術トレーニングコース
  • Invited
• [Presentation] 視交叉上核の神経細胞における細胞内カルシウムおよび時計遺伝子転写のリズムは低温により停止しリセットする (2023)
  • Author(s): 榎木亮介、金尚宏、山口良文、根本知己
  • Organizer: 日本神経科学学会大会
• [Presentation] Fundamentals of optical microscopy using multi-photon process induced by near-infrared ultra-short pulse laser light, (2023)
  • Author(s): Tomomi Nemoto
  • Organizer: Retreat for multidimensional analysis of memory mechanics
  • Int'l Joint Research / Invited
• [Presentation] Fluorescence Radial Fluctuation Enables Two-photon Super-Resolution Microscopy (2023)
  • Author(s): Tsutsumi M.、Takahashi T.、Kobayashi K.、Nemoto T.
  • Organizer: Retreat for multidimensional analysis of memory mechanics
  • Int'l Joint Research
• [Presentation] All-pulsed two-photon_STED microscopy for_brain tissue imaging (2023)
  • Author(s): Hirokazu Ishii、Kohei Otomo、Tomomi Nemoto
  • Organizer: Retreat for multidimensional analysis of memory mechanics
  • Int'l Joint Research
• [Presentation] 二光子励起過程を用いたレーザー走査型顕微鏡の基礎とバイオイメージングへの応用 (2023)
  • Author(s): 根本知己
  • Organizer: 光響レーザーセミナー
  • Invited
• [Presentation] 時空間蛍光相関解析による生体深部超解像観察法の確立 (2023)
  • Author(s): 堤 元佐、_橋泰伽、小林健太郎、根本知己
  • Organizer: AMED精神・神経疾患領域/早期ライフ連携推進Workshop
• [Presentation] 時空間蛍光相関解析による ２光子深部超解像イメージング (2023)
  • Author(s): 堤 元佐、_橋泰伽、小林健太郎、根本知己
  • Organizer: 生理学研究所研究会「細胞システム理解のためのシグナル応答原理解明の最前線」
  • Invited
• [Presentation] 全パルス式二光子 STED 顕微鏡による脳組織「ナノ」イメージング (2023)
  • Author(s): 石井宏和、大友康平、根本知己
  • Organizer: 第13回 名古屋大学医学系研究科・生理学研究所合同シンポジウム
  • Int'l Joint Research
• [Presentation] 時空間蛍光相関解析による２光子深部超解像イメージング (2023)
  • Author(s): 堤 元佐、_橋泰伽、小林健太郎、根本知己
  • Organizer: 名大医・生理研合同シンポジウム2023
• [Presentation] 全パルス式二光子 STED 顕微鏡による 生体深部「ナノ」イメージングへの展開 (2023)
  • Author(s): 石井宏和，大友康平、根本知己
  • Organizer: 第70回 中部日本生理学会
• [Presentation] 全パルス式二光子STED顕微鏡の開発と 脳組織「ナノ」イメージングへの展開 (2023)
  • Author(s): 石井宏和、大友康平、根本知己
  • Organizer: 第32回日本バイオイメージング学会学術集会
• [Presentation] Two-Photon Microscopy Advanced by Controlling Laser Light Beam via Liquid Crystal Devices (2023)
  • Author(s): Tomomi Nemoto、Hirokazu Ishii、Kohei Otomo
  • Organizer: the 16th International Symposium of Nanomedicine (ISNM2023)
  • Int'l Joint Research / Invited
• [Presentation] Fluorescence Radial Fluctuation Enables Two-photon Super-Resolution Microscopy (2023)
  • Author(s): Tsutsumi M.、Takahashi T.、Kobayashi K.、Nemoto T.
  • Organizer: The 7th Australia-Belgium-Japan joint symposium on excitonics and cellular communication
  • Int'l Joint Research / Invited
• [Presentation] レーザー光ビームの波面制御による多光子顕微鏡の高度化 (2023)
  • Author(s): 根本知己
  • Organizer: 極端紫外光研究施設40周年記念行事およびUVSORシンポジウム2023
  • Invited
• [Presentation] Liquid Crystal Devices for Controlling Laser Light and Advanced Multi-Photon Microscopy Imaging (2023)
  • Author(s): Tomomi Nemoto、Hirokazu Ishii、Kohei Otomo
  • Organizer: the 30th International Display Workshops (IDW ’23)
  • Int'l Joint Research / Invited
• [Presentation] 新規多光子顕微鏡による細胞生理のイメージング (2023)
  • Author(s): 根本知己
  • Organizer: 生理学研究会「多次元脳形態研究会」
  • Invited
• [Presentation] Comprehensive GABA imaging in the mouse retina (2023)
  • Author(s): Keisuke Yonehara
  • Organizer: Neuro2023
• [Presentation] Development of cell contact-mediated viral labeling technique (2023)
  • Author(s): Keisuke Yonehara
  • Organizer: The 2nd CJK International Meeting, Zhuhai China
• [Presentation] Use of AAV and genetically-modified rabies virus for visual circuit mapping (2023)
  • Author(s): Keisuke Yonehara
  • Organizer: ARVO SIGs
• [Presentation] Neural circuits for visually-mediated behaviors in the mouse (2023)
  • Author(s): Keisuke Yonehara
  • Organizer: BRI-DANDRITE Partnership Sympoium
• [Presentation] 視覚を介した摂食行動と防衛行動の神経回路 (2023)
  • Author(s): Keisuke Yonehara
  • Organizer: 第１００回記念大会日本生理学会大会
• [Presentation] 神経活動依存的な細胞標識とトランスクリプトーム解析を用いた神経ネットワーク解析 (2023)
  • Author(s): 久保 郁
  • Organizer: 第46回日本分子生物学会 2023年12月 口頭発表、招待有り
  • Invited
• [Presentation] Genetically targeted pretectal neurons constitute inhibitory circuits for optic flow processing (2023)
  • Author(s): Koji Matsuda, Carolina Fiallos Oliveros, Tomoya Shiraki, Koichi Kawakami, Fumi Kubo
  • Organizer: Cold Spring Harbor Laboratory Meeting, Zebrafish Neurobiology 2023 Nov. Poster
  • Int'l Joint Research
• [Presentation] Intersectional genetics for investigating putative pretectal hub neurons in the optic flow processing circuit (2023)
  • Author(s): Chung-Han Wang, Fumi Kubo.
  • Organizer: Cold Spring Harbor Laboratory Meeting, Zebrafish Neurobiology 2023 Nov. Poster
  • Int'l Joint Research
• [Presentation] Functional and morphological characterization of zebrafish retinal ganglion cell subtypes expressing the transcription factor Satb2 (2023)
  • Author(s): Ayjan Urazbayeva, Fumi Kubo.
  • Organizer: Cold Spring Harbor Laboratory Meeting, Zebrafish Neurobiology 2023 Nov. Poster
  • Int'l Joint Research
• [Presentation] Regulation of Neuronal Function by Extracellular Scaffold Proteins. US-Japan Joint Workshop on the Neurovascular Unit (2023)
  • Author(s): Yuzaki M.
  • Organizer: US-Japan Joint Workshop on the Neurovascular Unit
• [Presentation] Building principles of synapse nanoarchitecture revealed by expansion microscopy techniques. (2023)
  • Author(s): Nozawa K, Sogabe T, Hayashi A, Motohashi J, Miura E, Arai I, Yuzaki M.
  • Organizer: Max Planck Florida Institute for Neuroscience Biennial Neural Circuits Research Conference
• [Presentation] The Localization and function of Cbln1 in PNS. (2023)
  • Author(s): Takasugi S, Matsuda K, Yuzaki M.
  • Organizer: UK-Japan Neuroscience Symposium, Cardiff, UK
• [Presentation] The role of Cblnl in age-related hearing loss: insights from Cblnl knockout. (2023)
  • Author(s): Shiozaki M.
  • Organizer: UK-Japan Neuroscience Symposium, Cardiff, UK
• [Presentation] Exploring novel therapeutic strategies targeting synapses through synaptic organizers. (2023)
  • Author(s): Yuzaki M.
  • Organizer: A symposium on Neuroscience of dementia. Lee Kong Chian School of Medicine, Singapore, 2023
• [Presentation] Bridge over troubled synapses; exploring novel therapeutic strategies for synaptopathy (2023)
  • Author(s): Yuzaki M.
  • Organizer: Duke-NUS symposia, Singapore, 2023.
• [Presentation] The C1q family synaptic organizers: Unlocking therapeutic potential for neuropsychiatric and neurological disorders (2023)
  • Author(s): Yuzaki M.
  • Organizer: BBB seminar, University of Bergen, Norway, 2024.
• [Remarks] https://plaza.umin.ac.jp/mamm/en/
• [Patent(Industrial Property Rights)] 光学部材とその製造方法 (2023)
  • Inventor(s): 高橋泰伽、根本知己、岡村陽介
  • Industrial Property Rights Holder: 高橋泰伽、根本知己、岡村陽介
  • Industrial Property Rights Type: 特許
  • Filing Date: 2023
• [Funded Workshop] Retreat for Multidimensional Analysis of Memory Mechanisms (2023)