| Project/Area Number |
22KF0065
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| Project/Area Number (Other) |
22F20801 (2022)
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Multi-year Fund (2023)
Single-year Grants (2022) |
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| Section | 外国 |
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| Review Section |
Basic Section 49070:Immunology-related
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| Research Institution | Chiba University |
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Principal Investigator |
藤橋 浩太郎 (2023) 千葉大学, 医学部附属病院, 特任教授 (50820354)
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| Co-Investigator(Kenkyū-buntansha) |
TSAI JIA-YUN 千葉大学, 医学部附属病院, 外国人特別研究員
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| Host Researcher |
藤橋 浩太郎 (2022) 千葉大学, 医学部附属病院, 特任教授 (50820354)
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| Foreign Research Fellow |
TSAI JIA-YUN 千葉大学, 医科学研究所, 外国人特別研究員
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| Project Period (FY) |
2023-03-08 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2023: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 2022: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Vaccine / influenza virus / IgA / Protection / Nasal Vaccine / Influenza virus |
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| Outline of Research at the Start |
We will assess whether PilVax nasal vaccine induces antigen (M2e)-specific antibody responses in both mucosal and systemic compartments. When significant titers of M2e-specific mucosal IgA and serum IgG antibody responses are noted, we will test the functional properties of the M2e-specific antibodies by challenging vaccinated mice with influenza virus strains (H1N1, e.g., A/California and H3N2, e.g., A/Guizhou) to confirm protective immunity.
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| Outline of Annual Research Achievements |
Age-matched female BALB/c mice were immunized with of PilVax vaccines (1 X 10^8 CFU, in a volume of 10 ul) presenting two tandem copies (M2e-M2e-PilVax) of the M2e peptide. The vaccines were administered on 3 consecutive days with additional 3 boosters given 2 weeks apart, for a total of 12 doses. One week after the final immunization (day 51 or 49), serum, bronchoalveolar lavage (BAL) nasal washes (NWs) and saliva were collected and subjected to M2e-specific ELISAs. Significantly increased levels of M2e-specific serum IgG and mucosal IgA antibody responses were noted. When vaccinated mice were challenged with influenza virus (H1N1, e.g., A/California) in order to confirm protective immunity, nasal PilVax immunized mice showed complete protection from the virus challenge.
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