| Project/Area Number |
22KF0066
|
|---|
| Project/Area Number (Other) |
22F22047 (2022)
|
|---|
| Research Category |
Grant-in-Aid for JSPS Fellows
|
| Allocation Type | Multi-year Fund (2023)
Single-year Grants (2022) |
|---|
| Section | 外国 |
|---|
| Review Section |
Basic Section 90110:Biomedical engineering-related
|
|---|
| Research Institution | Chiba University |
|---|
Principal Investigator |
武居 昌宏 千葉大学, 大学院工学研究院, 教授 (90277385)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SIFUNA MARTIN 千葉大学, 大学院工学研究院, 外国人特別研究員
|
|---|
| Project Period (FY) |
2023-03-08 – 2025-03-31
|
| Project Status |
Granted (Fiscal Year 2023)
|
| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2024: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2023: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2022: ¥200,000 (Direct Cost: ¥200,000)
|
|---|
| Keywords | lab-on-a-CD hardware / Electrical measurements / Malaria / Thrombus / Lab-on-a-CD / electrical measurements |
|---|
| Outline of Research at the Start |
体外循環血流内の血栓検出法は、実際のヒトや動物手術中の体外式膜型人工肺(ECMO)や人工透析装置などの体外循環流路内を対象とした革新的な技術として注目が高い。既に研究を行っている先進的なタンパク質の電気可視化計測法の基盤技術を、完全非接触のキャパシタンストモグラフィーへ展開することにより、体外循環流路の血栓検出技術の実用化の可能性が高まっている。その実用化には、I:高感度センサ開発とⅡ:動物テストが不可欠であり、本共同研究にて実施する。
|
| Outline of Annual Research Achievements |
A versatile, lab-on-a-CD hardware was developed to quantify cell volume fraction (VF) by electrical impedance spectroscopy in diverse conditions such as in monitoring thrombus formation in extra corporeal circuits (ECC) and in quantifying erythrocyte sedimentation rates also for thrombus monitoring and quantifying volume fraction of plasmodium infected RBC in malaria diagnosis. The hardware was initially validated in Chiba University by quantifying red blood cells (RBC) VF in diverse conditions. Measured impedance was analyzed by distribution of relaxation time analysis and regression modelling to correlate amplitude maxima and VF. At characteristic relaxation time τc = 1×10-5s, differences in amplitude maxima before and after centrifugation were statistically significant (p<0.05) with centrifugation increasing correlation between amplitude maxima and VF from 0.3703 to 0.7785. In 15 minutes of red blood cells sedimentation, differences in amplitude maxima or red blood cells sedimentation length in media with different protein concentrations were also statistically significant (p < 0.05). VF quantification accuracy was tested using an extra corporeal circuit where RBC VF was measured by developed hardware with 0.73 % error relative to standard microcentrifuge method. The amplitude maxima also strongly correlates with RBC VF in ECC for thrombus detection. The importance of these results is the demonstration of the hardware’s potential for low cost quantification of RBC VF fractions in thrombus detection or malaria diagnosis which will be followed by field analysis.
|
| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The proposed research project is progressing smoothly because as at now, we have completed the development as well as the validation assessment of the functioning of the lab-on-CD hardware for quantification of cell volume fractions in static cell to cell or flowing cell to liquid mixtures towards disease diagnosis and treatment. The in vitro validation assessment of the functioning of the developed hardware was done by quantifying the volume fraction red blood cells in flow in extra corporeal circuits (ECC) for early detection of thrombus. The in vitro validation assessment of the functioning of the developed hardware was also done by quantifying the nucleated red blood cells among non nucleated red blood cells to simulate malaria detection using the developed hardware. So far, we have generated a manuscript from initial stages of this study and we have submitted it to the journal for possible publication. The manuscript is now under review for possible publication in a journal.
|
| Strategy for Future Research Activity |
The plan was to develop a versatile, centrifuge-coupled lab-on-a-CD hardware is developed for high through-put quantification of cell volume fractions which we have done, drafted and submitted a manuscript. Once the submitted manuscript is accepted for publication, our next plan it to apply the developed hardware for more research activities in the hospital settings for the quantifying the red blood cells volume fractions in extra corporeal circuits (ECC) during surgery in detection of thrombus as the major objective. since it is a versatile hardware, we also plan to widen the frequency range of the developed hardware from between 1kHz and 100kHz so as to cover a range of between 1kHz and 10 MHz so that it can be used in the field for quantifying the parasitized red blood cells volume fractions in the detection of malaria infections. This will enable the use of the developed hardware in diverse disease diagnostic conditions and not just thrombus detection. This will involve collaborative research with the Kenya team for trial using real plasmodium parasites in the endemic regions of Kenya.
|
