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Study of marine carbon cycle based on stable carbon isotopic compositions of amino acids in marine sediments

Research Project

Project/Area Number 22KJ0593
Project/Area Number (Other) 21J20841 (2021-2022)
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeMulti-year Fund (2023)
Single-year Grants (2021-2022)
Section国内
Review Section Basic Section 17050:Biogeosciences-related
Research InstitutionThe University of Tokyo

Principal Investigator

孫 語辰  東京大学, 理学系研究科, 特別研究員(PD)

Project Period (FY) 2023-03-08 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2023: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2022: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2021: ¥800,000 (Direct Cost: ¥800,000)
KeywordsAmino acid / Carbon cycle / Marine sediment / Isotope analysis / amino acid / marine sediment / microbial reworking / organic matter / diagenesis / carbon isotope
Outline of Research at the Start

In this research, I will isolate amino acids from marine sediments, and determine their concentrations and carbon isotopic compositions. Using amino acids as representative materials, I would like to reveal the dynamics of organic matter preservation and degradation under the sea floor.

Outline of Annual Research Achievements

In this research, I studied the organic matter cycling in marine sediments based on carbon isotope ratios of amino acids. In the first year, I developed a new analytical method for the determination of carbon and nitrogen isotopic compositions of amino acids in environmental samples. Using the new method, high-accuracy analysis of amino acids in marine sediment samples can be achieved. Then, I determined carbon isotopic compositions of amino acids in several surface and sub-surface sediment samples taken from the Japan Sea and off-Boso Peninsula. Results show that carbon isotopic composition patterns of amino acids in sediment samples are very similar, regardless of their different deposition histories and environmental conditions. It shows that organic matter in these sediments have similar sources. On the other hand, a baseline shift of carbon isotopic composition patterns of amino acids indicates that syntheses of organic matter using the bottom dissolved inorganic carbon happened. It demonstrates the contribution of organic matter production in the deep sea. Besides the isotope analysis of individual amino acids, I have also developed a new method for the isotope analysis of individual L- and D-amino acids. In the future, this new method can be applied to determine the isotope ratios of D-amino acids in marine sediments, which can be used to study the microbial sources and transformations of organic matter in marine sediments. Overall, this research expanded our understanding of organic matter cycling in the deep ocean.

Report

(3 results)
  • 2023 Annual Research Report
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (8 results)

All 2023 2022 2021

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (6 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Application of a porous graphitic carbon column to carbon and nitrogen isotope analysis of underivatized individual amino acids using high‐performance liquid chromatography coupled with elemental analyzer/isotope ratio mass spectrometry2023

    • Author(s)
      Sun Yuchen、Ogawa Nanako O.、Ishikawa Naoto F.、Blattmann Thomas M.、Takano Yoshinori、Ohkouchi Naohiko
    • Journal Title

      Rapid Communications in Mass Spectrometry

      Volume: 37 Issue: 17

    • DOI

      10.1002/rcm.9602

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Integrative assessment for amino acid nitrogen isotopic composition in biological tissue samples determined by GC/C/IRMS, LC x EA/IRMS, and LC x GC/C/IRMS.2022

    • Author(s)
      Ishikawa, N. F., Ogawa, N. O., Sun, Y., Chikaraishi, Y., Takano, Y., and Ohkouchi, N.
    • Journal Title

      Limnology and Oceanography, Method

      Volume: 20 Issue: 9 Pages: 531-542

    • DOI

      10.1002/lom3.10502

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] A new method for enantiomer-specific carbon and nitrogen isotope analyses of underivatized amino acids2023

    • Author(s)
      Sun Yuchen、Blattmann Thomas M.、Ishikawa Naoto F.、Ogawa Nanako O.、Kuroda Junichiro、Takano Yoshinori、Ohkouchi Naohiko
    • Organizer
      Japan Geoscience Union Meeting 2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] New method and perspectives of amino acid enantiomer-specific isotope analysis2023

    • Author(s)
      Sun Yuchen、Blattmann Thomas M.、Ishikawa Naoto F.、Ogawa Nanako O.、Takano Yoshinori、Ohkouchi Naohiko
    • Organizer
      第40回有機地球化学シンポジウム
    • Related Report
      2023 Annual Research Report
  • [Presentation] Microbial Reworking of Organic Matter in Marine Sediments Revealed by Carbon Isotopic Compositions of Individual Amino Acids2022

    • Author(s)
      孫語辰, 小川奈々子, 石川尚人, 黒田潤一郎, 高野淑識, 大河内直彦
    • Organizer
      日本地球化学会 第69回年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Enantiomer-specific carbon isotope analysis of amino acids: New method and perspectives2022

    • Author(s)
      Yuchen Sun, Thomas M. Blattmann, Naoto F. Ishikawa, Nanako O. Ogawa, Yoshinori Takano, Naohiko Ohkouchi
    • Organizer
      International Symposium on Isotope Physiology, Ecology, and Geochemistry 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development and applications of a liquid chromatography method for the compound- and enantiomer-specific stable carbon isotope analysis of amino acids in environmental samples2021

    • Author(s)
      Yuchen Sun, Naoto F. Ishikawa, Thomas M. Blattmann, Nanako O. Ogawa, Yoshinori Takano, Naohiko Ohkouchi
    • Organizer
      Ocean Science Meeting 2022
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A new assessment of amino acid stable carbon isotope compositions in marine organisms: implications for food web studies2021

    • Author(s)
      Yuchen Sun, Naoto F. Ishikawa, Nanako O. Ogawa, Yoshinori Takano, Naohiko Ohkouchi
    • Organizer
      第69回日本生態学会大会
    • Related Report
      2021 Annual Research Report

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Published: 2021-05-27   Modified: 2024-12-25  

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