| Project/Area Number |
22KJ0894
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| Project/Area Number (Other) |
22J13110 (2022)
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Multi-year Fund (2023)
Single-year Grants (2022) |
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| Section | 国内 |
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| Review Section |
Basic Section 43010:Molecular biology-related
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| Research Institution | Institute of Physical and Chemical Research (2023)
The University of Tokyo (2022) |
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Principal Investigator |
韓 佩恂 国立研究開発法人理化学研究所, 開拓研究本部, 特別研究員(PD)
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| Project Period (FY) |
2023-03-08 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2023: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2022: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Ribosome / Translation |
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| Outline of Research at the Start |
During translation process, genetic information in mRNA transcripts is converted into protein. It is known that cells utilize specific mechanism to regulate gene expression at translation level. One case during translation is when a translating ribosome collides into a downstream ribosome, forming a ribosome collision. However, it remains unclear how ribosome collisions are utilized by cells to regulate translation. Thus, this project focuses on investigating the endogenous features of ribosome collision to further dissect the role of collided ribosomes during translation.
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| Outline of Annual Research Achievements |
Translation, as a fundamental step in gene expression, requires extensive regulation. Recent studies have shown that the trigger of ribosome-associated quality control is the collision between two translating ribosomes. However, it is not clear whether endogenous mRNA sequences could also trigger quality control.
By deep-sequencing the ribosome footprints protected by collided ribosomes prior to rescue, we determined a specific feature in the mRNA sequence that contributed to its recognition by ribosome-associated quality control. Further analysis through reporter assay also validated our findings from the analysis.
These results combined provided crucial evidences regarding co-translational quality control pathways in higher eukaryotic cells.
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