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Sec14様タンパク質による低リン環境での植物成長の改善の分子メカニズムの解明

Research Project

Project/Area Number 22KJ1024
Project/Area Number (Other) 22J20864 (2022)
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeMulti-year Fund (2023)
Single-year Grants (2022)
Section国内
Review Section Basic Section 38010:Plant nutrition and soil science-related
Research InstitutionThe University of Tokyo

Principal Investigator

楊 麥倫  東京大学, 農学生命科学研究科, 特別研究員(DC1)

Project Period (FY) 2023-03-08 – 2025-03-31
Project Status Granted (Fiscal Year 2023)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2024: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2023: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2022: ¥900,000 (Direct Cost: ¥900,000)
KeywordsSec14-like proteins / phosphorus / phosphate uptake / phosphate deficiency / rice / Sec14-like protein
Outline of Research at the Start

LPU1, encoding chloroplast-localized phospholipid transfer protein, was identified as a key regulator of phosphate acquisition in plants. The aim of this study is to elucidate the physiological functions of Arabidopsis and rice LPU1 homologs in the regulation of Pi acquisition and use.

Outline of Annual Research Achievements

In the first year, I found that AtLPU1 promoter of 6 Arabidopsis accessions containing many polymorphisms (SNPs) had remarkably stronger activity than Col.0., suggesting SNPs found in promoters might be responsible for higher AtLPU1 expression and Pi uptake activity in corresponding accessions. Also, I measured the contents of several lipids in the leaf and chloroplast of WT, oslpu1-KO and OsLPU1-OX seedlings grown under control and low Pi conditions. As a result, Pi deficiency-induced lipid remodeling was alleviated in oslpu1-KO samples indicating OsLPU1 plays a vital role in regulating lipid synthesis in the chloroplasts in response to Pi availability. The relevant results were published in Plant Physiol.
In the second year, to understand the role of LPU1 in regulating lipid accumulation, I compared the phenotypes of atlpu1-KO and the null knockout mutants of lipid biosynthesis genes grown under control and low Pi conditions. The knockout mutants of galactolipid synthesis enzymes showed similar phenotypes with atlpu1-KO seedlings, indicating that AtLPU1 is involved in the reduction of glycolipid synthesis, leading to the reduced Pi uptake activity in roots. I subsequently performed BiFC and yeast two-hybrid assays and found AtLPU1 can directly interact with four enzymes involved in phosphatidic acid (PA) synthesis. Based on these findings, I currently hypothesize that AtLPU1 binds to PA to transfer PA to AtLPPγ for diacylglycerol synthesis. Therefore, in the third year, the interaction between AtLPU1 and PA or enzymes in lipid synthesis should be further investigated.

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

As mentioned above, I have carried out most of the experiments planned for the initial two years and have successfully demonstrated that the function of chloroplastic Sec14-like protein LPU1 and its homolog in rice, OsLPU1, played a vital role in plant growth, especially under phosphate deficient condition. Both LPU1 and OsLPU1 were involved in Pi acquisition. OsLPU1 plays a vital role in chloroplast lipid remodeling in response to Pi availability. Related results were summarized in the paper published on Plant Physiology (https://doi.org/10.1093/plphys/kiad212). On the other hand, I have started to identify the role of LPU1 in lipid metabolism. I found the phenotype of galactolipid synthesis enzyme genes knockout mutants was similar to atlpu1-KO seedlings. Also, I confirmed that AtLPU1 can directly interact with phosphatidic acid (PA) metabolism enzymes. I currently speculated that AtLPU1 interacts with lipid synthesis enzymes to be involved in the reduction of glycolipid synthesis, leading to reductions in Pi uptake activity in roots.
Based on these facts, this research project is progressing well according to the original plan for the last year. The experiments planed to reveal the function of LPU1 in phospholipid metabolism will be continued in the last years.

Strategy for Future Research Activity

In the current study, I found that AtLPU1 directly interacts with phosphatidic acid metabolism enzymes. Also, AtLPU1 is involved in the reduction of glycolipid synthesis, leading to reductions in Pi acquisition in roots. However, it remains unclear the molecular basis that what is the role of AtLPU1 in regulating lipid accumulation in chloroplast and further affects the Pi acquisition in roots. Therefore, I will try to identify the binding mode of AtLPU1 and lipid synthesis enzymes and the role of AtLPU1 in lipid biosynthesis. On the other hand, I will explore the potential signaling pathway transmitting chloroplast signaling to roots. The related experiments described in the research plan will be performed in the next years.

Report

(2 results)
  • 2023 Research-status Report
  • 2022 Annual Research Report
  • Research Products

    (10 results)

All 2023 2022 Other

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (5 results) (of which Int'l Joint Research: 2 results) Remarks (2 results)

  • [Journal Article] Chloroplastic Sec14-like proteins modulate growth and phosphate deficiency responses in Arabidopsis and rice2023

    • Author(s)
      Mailun Yang, Yasuhito Sakruaba, Toshiki Ishikawa, Namie Ohtsuki, Maki Kawai-Yamada, Shuichi Yanagisawa
    • Journal Title

      Plant Physiology

      Volume: 192 Issue: 4 Pages: 3030-3048

    • DOI

      10.1093/plphys/kiad212

    • Related Report
      2023 Research-status Report 2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] CRISPR/Cas9-mediated elimination of OsHHO3, a transcriptional repressor of three AMMONIUM TRANSPORTER1 genes, improves nitrogen use efficiency in rice2023

    • Author(s)
      Kexin Liu, Yasuhito Sakuraba, Namie Ohtsuki, Mailun Yang, Yoshiaki Ueda, Shuichi Yanagisawa
    • Journal Title

      Plant Biotechnology Journal

      Volume: 21 Issue: 11 Pages: 2169-2172

    • DOI

      10.1111/pbi.14167

    • Related Report
      2023 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Arabidopsis NLP7-HB52/54-VAR2 pathway modulates energy utilization in diverse light and nitrogen conditions2022

    • Author(s)
      Ariga Takuto、Sakuraba Yasuhito、Zhuo Mengna、Yang Mailun、Yanagisawa Shuichi
    • Journal Title

      Current Biology

      Volume: 32 Issue: 24 Pages: 5344-5353

    • DOI

      10.1016/j.cub.2022.10.024

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Involvement of Chloroplastic Sec14-like Protein in the Regulation of Phosphorus Acquisition and Use2023

    • Author(s)
      Mailun Yang, Yasuhito Sakuraba, Toshiki Ishigawa, Namie Ohtsuki, Maki Kawai-Yamada, Shuichi Yanagisawa
    • Organizer
      Taiwan-Japan Plant Biology 2023
    • Related Report
      2023 Research-status Report
    • Int'l Joint Research
  • [Presentation] The role of OsHHO3 transcription repressor in controlling phosphorus acquisition in rice2023

    • Author(s)
      Mailun Yang, Kexin Liu, Yasuhito Sakuraba, Shuichi Yanagisawa
    • Organizer
      第64回日本植物生理学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Involvement of Chloroplastic Sec14-like Protein in the Regulation of Phosphorus Acquisition2022

    • Author(s)
      Mailun Yang
    • Organizer
      Global Innovation Workshop 2022
    • Related Report
      2022 Annual Research Report
  • [Presentation] Involvement of Chloroplastic Sec14-like Protein in the Regulation of Phosphorus Acquisition and Use2022

    • Author(s)
      Mailun Yang, Yasuhito Sakuraba, Toshiki Ishigawa, Namie Ohtsuki, Maki Kawai-Yamada, Shuichi Yanagisawa
    • Organizer
      19th International Symposium on Rice Functional Genomics or ISRFG 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Involvement of Chloroplastic Sec14-like Protein in the Regulation of Phosphorus Acquisition and Use2022

    • Author(s)
      Mailun Yang, Yasuhito Sakruaba, Toshiki Ishikawa, Namie Ohtsuki, Maki Kawai-Yamada, Shuichi Yanagisawa
    • Organizer
      植物の栄養研究会第7回研究交流会・第11回AgTechセミナー
    • Related Report
      2022 Annual Research Report
  • [Remarks] 東京大学農学生命科学研究科 植物機能工学部門ホ ー ム ペ ー ジ

    • URL

      https://webpark1561.sakura.ne.jp/home/index.html

    • Related Report
      2023 Research-status Report
  • [Remarks] 東京大学農学生命科学研究科附属アグロバイオテクノロジー研究センター植物機能工学部門ホームページ

    • URL

      http://park.itc.u-tokyo.ac.jp/ppk/home/index.html

    • Related Report
      2022 Annual Research Report

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Published: 2022-04-28   Modified: 2024-12-25  

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